Analysis of Kras gene from induced pancreatic cancer rats administered with Momordica charantia and Ocimum ba s ilicum leaf extracts

Abstract

ObjectiveTo analyze K-ras gene from induced pancreatic cancer rats administered with Momordicacharantia and Ocimumbasilicum leaf extracts.MethodsTwenty-five (25) adult rats weighing between 90–120 g were divided into 5 groups namely RA, RB, RC, NC and PC, each group had 5 rats. The PC which served as the control was fed with normal fish meal and water ad libitum; the NC which is the negative control received 20 mg/ml/week of Nitrosamines only while other groups received different concentrations of aqueous extract of both M.charantia and O.basilicum (200 mg, 100 mg, 50 mg) and Nitrosamine. Qualitative phytochemical screening of the aqueous extract of both M.charantia and O.basilicum was carried out. The extraction of DNA was done using Jena Bioscience DNA preparation kit and the protocol was based on the spin column based genomic DNA purification from blood, animal and plant cells. Agarose gel electrophoresis was used to analyze the K-ras gene extracted from the pancreas tissues of experimental rats while hematoxylinand eosin staining was used for histological assay.ResultsPhytochemical screening revealed the presence of alkaloids, tannins, flavonoids, saponins and glycosides in M.charantia while saponins, tannins and glycosides were discovered in O.basilicum. Significant reduction in the weight of rats treated with 200 mg of aqueous extracts of M.charantia and O.basilicum while rats that were dosed with nitrosamines only showed a slight increase in weight in the first three weeks when compared to the positive control. Histological studies revealed that there is both enlargement and reduction in the islet cell size, with one of the sections showing a normal islet cell size. While the agarose gel electrophoresis revealed that there may be possibility of prevention of damage to k-ras gene as a result of the effect of plants extract.ConclusionThis work has shown that the leaf extracts of both M.charantia and O.basilicum will serve as a measure against induced pancreatic cancer in rats.

Integration of medicinal plants into the traditional system of medicine for the treatment of cancer in Sokoto State, Nigeria

“…(2015) ; Krishnamurthy et al. (2014) ; lung, breast O. basilicum Minari et al. (2018) ; Torres et al.…”

Momordicoside G Regulates Macrophage Phenotypes to Stimulate Efficient Repair of Lung Injury and Prevent Urethane-Induced Lung Carcinoma Lesions

“…It has been proved that M. charantia and its extracts can modulate AKT/mTOR/p70S6K signaling, cell cycle regulatory proteins and apoptosis-associated proteins in different cancers (Farooqi et al, 2018). Minari et al (2018) revealed that there may be possibility of prevention of damage to k-ras gene as a result of the effect of M. charantia extract. In this study, we try hard to explain the mechanism by which momordicoside G regulates macrophage phenotypes using bioinformatics analysis and found that the potential targets of momordicoside G regulating macrophages were primarily associated with the “signal transduction,” “innate immune response,” “cell proliferation,” “protein phosphorylation,” and “apoptotic process,” indicating a function of momordicoside G targeting multi-protein network, such as “TNF signaling pathway” and “RIG-I-like receptor signaling pathway,” whereby it regulates macrophage phenotypes to prevent the injury and cancerization.…”

Ocimum basilicum and Ocimum americanum: A Systematic Literature Review on Chemical Compositions and Antimicrobial Properties

“…23 Momordica charantia and Ocimum basilicum leaves cold maceration extraction by water for 72 h were used to protect rats against induced pancreatic cancer as it was approved by researchers in the analysis of the Kras gene in rats with pancreatic cancer that were given extracts from the leaves of Momordica charantia and Ocimum basilicum. 24 The impact of varying solvents and the number of extraction stages on the polyphenol concentration and antioxidant potential of extracts made from basil leaves (Ocimum basilicum L.) showed the concentration of phenolic compounds and the antioxidant activity of the extracts have a favorable association. 25 The impact of soil types on the chemical composition of purple basil essential oil, as determined by Clevenger apparatus essential oil extraction, demonstrated the influence of soil types on the chemical composition of Ocimum basilicum and Ocimum americanum.…”