Analysis of isoquinoline alkaloids from Mahonia leschenaultia and Mahonia napaulensis roots using UHPLC-Orbitrap-MSn and UHPLC-QqQLIT-MS/MS

Abstract

Mahonia leschenaultia (ML) and Mahonia napaulensis (MN) are less known and unexplored medicinal plants of the family Berberidaceae. They are used by the Todas of Nilgiris in their religious and medical practices but chemically less identified. Hence, we decided to do extensive phytochemical analysis to explore the potential of these plant extracts. An ultrahigh performance electrospray tandem mass spectrometry (UHPLC–ESI–MS/MS) method was successfully developed for qualitative analysis of the bioactive components in Mahonia species using Orbitrap Velos Pro mass spectrometer. Sixteen compounds were identified by comparison of their retention times and mass spectra (MS) with authentic standards and reported literature. Multi-stage mass spectra (MS2–8) for the identification of protoberberine and aporphine alkaloids showed the sequential expulsion of all the substituents attached with their basic skeleton followed by CO loss. Eight of the identified compounds (berberine, jatrorrhizine, palmatine, magnoflorine, isocorydine, glaucine, tetrahydropalmatine and tetrahydroberberine) were simultaneously determined by another UHPLC–ESI–MS/MS method under the multiple reactions monitoring (MRM) mode quantitatively using triple quadrupole linear ion trap mass spectrometer. The analytical method was validated for 8 bioactive compounds with overall recovery in the range 98.5%–103.6% (RSD≤2.2%), precise (RSD≤2.07%) and linear (r≥0.9995) over the concentration range of 0.5–1000 ng/mL and successfully applied in ML and MN roots, which suggests the suitability of the proposed approach for the routine analysis of Mahonia species and their quality control.

Green Extraction of Alkaloids and Polyphenols from Peumus boldus Leaves with Natural Deep Eutectic Solvents and Profiling by HPLC-PDA-IT-MS/MS and HPLC-QTOF-MS/MS

“…For compounds corresponding to peaks 9 and 10 in positive ionization mode, the molecular formulas of ions at m/z , generated by the sequential loss of two methyl radicals and 28 Da from -CO loss, respectively. These data and the comparison of elution order for standards in C-18 columns suggest that the identity of peaks 9 and 10 could unambiguously be assigned to isoboldine and boldine [29,31,33,34]. For compound corresponding to peak 15 in positive ionization mode, the molecular formula of ion at m/z 329.16323 was predicted as C 19 H 23 NO 4 .…”

“…Compounds were identified using the algorithm "Find by Formula" that evaluated the mass accuracy together with the isotopic relative abundance and isotopic separation. All the compounds were identified by the QTOF-MS and the MS/MS spectra acquired with the IT-MS and their absorption spectra in UV-visible region, as well as considering the data provided by literature [6,7,[29][30][31][32][33][34][35][36].…”

Berberine for prevention of dementia associated with diabetes and its comorbidities: A systematic review

“…In addition, it is unknown whether berberine, as a constituent of traditional medicine, rather than as an isolate, could exert the same effectiveness. Berberine is a natural alkaloid found in various medicinal plant species [36,[222][223][224][225][226][227][228][229] (Table 11). These medicinal plants could be useful in the prevention of dementia associated with metabolic disorders.…”

Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from Thalictrum foetidum, Berberis sp. and Chelidonium majus Extracts

“…Isoquinoline alkaloids were often determined in various plant extracts by the HPLC method. Most often, analysis were performed on C18 columns with mobile phases contained organic modifier, water, and most often addition of acids e.g., formic [19,20,21,22,23,24], rarely addition of salts, e.g., ammonium formate [25], buffer at acidic pH [26], and amines, such as trimethylamine [27,28].…”