Analysis of Isoflavone Daidzein in Puerariae radix with Micelle-Mediated Extraction and Preconcentration

Abstract

Nonionic surfactant oligo(ethylene glycol) monoalkyl ether (Genapol X-080) was employed as an alternative and effective solvent for the extraction of daidzein from Puerariae radix for the first time. Optimum experimental conditions were established. With 5% Genapol X-080 (w/v), liquid/solid ratio of 25:1 (mL/g), and ultrasonic-assisted extraction for 45 min, the extraction percentage of daidzein reached the highest value. For the preconcentration of daidzein by cloud-point extraction (CPE), sodium chloride was added to the solution to facilitate the phase separation and increase the preconcentration factor by reducing the volume of the surfactant-rich phase. The preconcentration factor for daidzein was about 13. Satisfactory results were obtained for the analysis of daidzein from P. radix with this established method.

Daidzin decreases blood glucose and lipid in streptozotocin-induced diabetic mice

“…Daidzin (DZ) is a major isoflavone glycoside from Puerariae radix, a herb widely used in Chinese medicinal formulae for the treatment of diabetes [7][8][9]. Many pharmacological studies have been made on DZ but its effect on blood glucose remains controversial.…”

“…Promoting glucose uptake by muscles is another effective way to decrease blood glucose [8], in which GLUT4 plays a key role. Glucose uptake assay in C2C12 myotubes showed that DZ significantly promoted glucose consumption by myotubes and enhanced GLUT4 membrane translocation as well, indicating that stimulating glucose uptake by muscles and enhancing GLUT4 membrane translocation may involve in the blood glucose-lowering action of DZ.…”

“…In the current circumstances, a search for an antidiabetic herbal product with fewer side effects has gained considerable importance in the medical field [6]. Daidzin (DZ) is a major isoflavone glycoside obtained from Puerariae radix, a well-known Chinese herbal medicine for the treatment of diabetes and cardiovascular diseases [7][8][9]. Various pharmacological activities of DZ have been reported including antioxidant [10], estrogenic [11] and anti-inflammatory [12].…”

Screening and Identification of Antioxidant Components in the Extract of Puerariae radix Using HPLC Coupled with MS

“…Puerarin, daidzin, and daidzein are the major isoflavonoids in its crude extract (Chen et al 2001;Prasain et al 2003Prasain et al , 2007. Several reports have focused on extraction and pharmacological actions of P. radix (He et al 2005;Guo et al 2001). But the antioxidant activity and its antioxidant component(s) have not been studied.…”

Ultrasonication assistance increases the efficiency of isoflavones extraction from kudzu (Pueraria lobata Ohwi) roots waste

“…In particular, the daidzein (Fig. 1), as a major isoflavone, was shown as an antigiardial activity, antioxidant action, and potential antidiabetic properties [5]. Recently kudzu roots have been prepared in the form of healthy drinks and dietary supplements that are consumed by the public, it is critical to determine their isoflavone content and concentration [6].…”