ANALYSIS OF IgE-BINDING ALLERGENS IN CULEX QUINQUEFASCIATUS SALIVA PROTEIN IN MOSQUITO BITE ALLERGIC PATIENTS

Wongkamchai, Sirichit; Techasintana, Patsharaporn; Wisuthsarewong, Wanee; Kulthanan, Kanokvalai; Suthipinittharm, Puan; Eakpo, Patama

doi:10.1016/s1081-1206(10)60698-9

Cited by 13 publications

(16 citation statements)

References 5 publications

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“…Se han observado alérgenos en la saliva de C. quinquefasciatus con pesos moleculares de 35,5, 32,5 y 22,5 kDa, con una frecuencia de reacción a la IgE de 41, 28 y 24 %, respectivamente, en el momento de evaluar el suero de individuos alérgicos (80).…”

Section: Culexunclassified

Papular urticaria: A review of causal agents in Colombia

et al. 2016

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López: análisis bioinformático y datos experimentales inéditos Todos los autores contribuyeron en igual medida a la redacción del manuscrito. La urticaria papular es una enfermedad alérgica causada por la picadura de insectos, la cual predomina en el trópico. El objetivo de esta revisión fue profundizar en sus aspectos epidemiológicos e inmunológicos, particularmente con base en datos publicados en Latinoamérica. Se hizo una revisión no sistemática mediante la búsqueda electrónica de artículos sobre la epidemiología de la urticaria papular, las características entomológicas de los agentes causales y los mecanismos inmunológicos asociados. Según los diversos reportes de centros médicos de Latinoamérica la urticaria papular es frecuente; el único estudio de prevalencia publicado indica que afecta a una cuarta parte de los niños escolares de Bogotá. Hay información sobre la relación causal entre la exposición domiciliaria a la pulga, la pobreza y la urticaria papular en Bogotá, una ciudad representativa de las altitudes andinas. No hay estudios que indaguen directamente sobre los insectos causales en zonas cálidas, aunque se sospecha clínicamente de los mosquitos Aedes aegypti y Culex quinquefasciatus. En cuanto a su patogenia, se destaca la participación de mecanismos celulares que involucran las células colaboradoras Th2, lo cual explica que sea una condición de hipersensibilidad retardada. El papel de la inmunoglobulina E (IgE) en la urticaria papular no está tan claro. Se desconocen los antígenos derivados de los insectos que causan la enfermedad, aunque se plantea que existen moléculas comunes de reacción cruzada entre los insectos, tales como el alérgeno Cte f 2 en la pulga, y sus homólogos en los mosquitos. La urticaria papular es una condición frecuente en Latinoamérica que debe investigarse en profundidad. La caracterización inmunológica de los componentes moleculares que causan esta condición puede resolver interrogantes sobre su etiología y su patogenia. Urticaria papular y sus agentes causales en ColombiaPalabras clave: urticaria; insectos; Aedes; Culex; Ctenocephalides; hipersensibilidad. doi: http://dx.doi.org/10.7705/biomedica.v36i4.3258 Papular urticaria: A review of causal agents in ColombiaPapular urticaria is a chronic allergic reaction induced by insect bites, which is common in the tropics. The objective of this review was to deepen on epidemiological and immunological aspects of this disease, focused on data published in Latin American countries. We conducted a non-systematic review of the literature through electronic search on the epidemiology of papular urticaria, the entomological characteristics of the causative agents and associated immunological mechanisms. Several reports from medical centers suggest that papular urticaria is common in Latin America. Only one epidemiological survey designed to estimate prevalence of papular urticaria has been published, reporting that about a quarter of children under six years of age is affected by this condition in Bogotá. There is evidence on the ca...

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Section: Culexunclassified

Papular urticaria: A review of causal agents in Colombia

et al. 2016

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“…Sixteen IgE-reactive proteins with a molecular weight of between 16 and 95 kDa have been detected in the saliva and salivary glands of 10 different mosquito species distributed worldwide [44]. When analyzed with sera from allergic individuals, allergens in the saliva of C. quinquefasciatus with a molecular weight of 35.5, 32.5 and 22.5 kDa showed a frequency of IgE reactivity of 41, 28 and 24%, respectively [67]. Fourteen IgE reactivity fractions with a molecular weight of between 23 and 93 kDa were identified using sera from allergic patients in extracts from the salivary glands of Aedes togoi , Culex tritaeniorhynchus and C. pipiens pallens .…”

Section: Mosquito Allergensmentioning

confidence: 99%

Immunological Aspects of the Immune Response Induced by Mosquito Allergens

Cantillo

Fernández-Caldas

Puerta

2014

Int Arch Allergy Immunol

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Allergies caused by mosquito bites may produce local or systemic reactions. The inhalation of mosquito allergens may also cause asthma and/or allergic rhinoconjunctivitis in sensitized individuals. The mechanisms implicated in the development of these immune responses involve IgE antibodies, different subtypes of IgG and proinflammatory cytokines as well as basophils, eosinophils and mast cells. Several allergenic components have been identified in the saliva and bodies of mosquitoes and some of these are present in different mosquito species. The most common species implicated in allergic reactions belong to the genera Aedes, Culex and Anopheles. Several Aedes aegypti allergens have been cloned and sequenced. The recombinant molecules show IgE reactivity similar to that of the native allergens, making them good candidates for the diagnosis of mosquito allergies. Allergen-specific immunotherapy with mosquito extracts induces a protective response characterized by a decreased production of IgE antibodies, increased IgG levels, a reduction in the severity of cutaneous and respiratory symptoms and the need for medication. The aims of this review are to summarize the progress made in the characterization of mosquito allergens and discuss the types of immune responses induced by mosquito bites and the inhalation of mosquito allergens in atopic individuals.

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“…COX-1 is constitutively expressed in most tissues, whereas COX-2 is highly inducible in response to a variety of stimuli, suggesting that COX-2 may play a central role in the inflammatory process and carcinogenesis. However, transgenic mice that overexpressed COX-2 in the epidermis were resistant to tumor development [1] and mice deficient in either COX-1 or COX-2 showed reduced skin carcinogenesis [2]. To identify critical proteins modulated by COX-2, we performed a two-dimensional gel electrophoresis (2-DE) followed by a MALDI-TOF analysis, because the proteomic profiling technique helps in approaching the comprehensive characterization of molecular events.…”

Section: Kanokvalai Kulthananmentioning

confidence: 99%

“…The potential clinical relevance of the test as an in vitro diagnostic tool for mosquito bite allergy was evaluated and compared to the previously established immuno-blot assay [2].…”

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confidence: 99%