A dot-blot and immuno-blot assay for the detection of mosquito saliva specific IgE in mosquito bite allergic subjects

Wongkamchai, Sirichit; Vaiyavatjamai, Premjit; Wanachiwanawin, Darawan; Wisuthsarewong, Wanee; Kulthanan, Kanokvalai; Eakpo, Patama

doi:10.1016/j.jdermsci.2008.08.013

Cited by 3 publications

(2 citation statements)

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“…Reunala, et al, encontraron frecuencias similares de unión a la IgE entre pacientes con urticaria papular y controles provenientes de zonas tropicales (Kenia y México), a diferencia de lo que observaron en pacientes con hipersensibilidad a la picadura de mosquito de un país con un clima entre templado y frío (Finlandia), en donde sí fue clara la diferencia estadística entre sanos y enfermos (45). Al igual que en este estudio, en otros se ha demostrado que en latitudes no tropicales se registraba una clara diferencia en la reacción de la IgE entre sanos y pacientes con hipersensibilidad (58,59). En contraste, en Egipto, otro país ubicado principalmente en el trópico, no se encontraron diferencias significativas entre el reconocimiento de la IgE de las bandas del extracto de pulga o del de mosquito (60).…”

Section: Respuesta Humoralunclassified

Papular urticaria: A review of causal agents in Colombia

et al. 2016

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López: análisis bioinformático y datos experimentales inéditos Todos los autores contribuyeron en igual medida a la redacción del manuscrito. La urticaria papular es una enfermedad alérgica causada por la picadura de insectos, la cual predomina en el trópico. El objetivo de esta revisión fue profundizar en sus aspectos epidemiológicos e inmunológicos, particularmente con base en datos publicados en Latinoamérica. Se hizo una revisión no sistemática mediante la búsqueda electrónica de artículos sobre la epidemiología de la urticaria papular, las características entomológicas de los agentes causales y los mecanismos inmunológicos asociados. Según los diversos reportes de centros médicos de Latinoamérica la urticaria papular es frecuente; el único estudio de prevalencia publicado indica que afecta a una cuarta parte de los niños escolares de Bogotá. Hay información sobre la relación causal entre la exposición domiciliaria a la pulga, la pobreza y la urticaria papular en Bogotá, una ciudad representativa de las altitudes andinas. No hay estudios que indaguen directamente sobre los insectos causales en zonas cálidas, aunque se sospecha clínicamente de los mosquitos Aedes aegypti y Culex quinquefasciatus. En cuanto a su patogenia, se destaca la participación de mecanismos celulares que involucran las células colaboradoras Th2, lo cual explica que sea una condición de hipersensibilidad retardada. El papel de la inmunoglobulina E (IgE) en la urticaria papular no está tan claro. Se desconocen los antígenos derivados de los insectos que causan la enfermedad, aunque se plantea que existen moléculas comunes de reacción cruzada entre los insectos, tales como el alérgeno Cte f 2 en la pulga, y sus homólogos en los mosquitos. La urticaria papular es una condición frecuente en Latinoamérica que debe investigarse en profundidad. La caracterización inmunológica de los componentes moleculares que causan esta condición puede resolver interrogantes sobre su etiología y su patogenia. Urticaria papular y sus agentes causales en ColombiaPalabras clave: urticaria; insectos; Aedes; Culex; Ctenocephalides; hipersensibilidad. doi: http://dx.doi.org/10.7705/biomedica.v36i4.3258 Papular urticaria: A review of causal agents in ColombiaPapular urticaria is a chronic allergic reaction induced by insect bites, which is common in the tropics. The objective of this review was to deepen on epidemiological and immunological aspects of this disease, focused on data published in Latin American countries. We conducted a non-systematic review of the literature through electronic search on the epidemiology of papular urticaria, the entomological characteristics of the causative agents and associated immunological mechanisms. Several reports from medical centers suggest that papular urticaria is common in Latin America. Only one epidemiological survey designed to estimate prevalence of papular urticaria has been published, reporting that about a quarter of children under six years of age is affected by this condition in Bogotá. There is evidence on the ca...

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Section: Respuesta Humoralunclassified

Papular urticaria: A review of causal agents in Colombia

et al. 2016

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“…The dot-blot ELISA is a common technique used to detect the presence of IgE in real samples [7,8]. The assay replaces the plastic binding matrix with a nitrocellulose membrane (NC membrane) with higher adsorption qualities, and therefore, facilitates the binding of larger quantities of target proteins [9,10].…”

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Detection of immunoglobulin E using an aptamer based dot-blot assay

Wang

2013

Chin. Sci. Bull.

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A novel aptamer based dot-blot assay for the detection of immunoglobulin E (IgE) was developed. A biotinylated aptamer was employed as the bio-recognition element to specifically interact with the target protein immobilized onto a nitrocellulose membrane substrate. Avidin conjugated horseradish peroxidase was introduced onto the membrane through the biotin-avidin system to catalyze the hydrogen peroxide mediated oxidation of 3,3′,5,5′-tetramethylbenzidine, thereby producing the blue-colored insoluble product. The intensity of the dots increased as the concentration of IgE increased. The spot intensity was quantified using a simple portable instrument. A linear response relationship between the spot intensity and the concentration of IgE over the range of 50 nmol/L to 1 μmol/L was obtained. The detection limit for IgE using the aptamer-based assay was 2.89 nmol/L. This assay was found to discriminate IgE from non-target proteins such as thrombin, bovine serum albumin and immunoglobulin G. immunoglobulin E, dot-blot assay, aptamer, biotin-avidin system Citation:Wang Y X, Ye Z Z, Ying Y B. Detection of immunoglobulin E using an aptamer based dot-blot assay.

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Particularities of allergy in the Tropics

Caraballo

Zakzuk

Lee

et al. 2016

World Allergy Organization Journal

119

145

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Allergic diseases are distributed worldwide and their risk factors and triggers vary according to geographical and socioeconomic conditions. Allergies are frequent in the Tropics but aspects of their prevalence, natural history, risk factors, sensitizers and triggers are not well defined and some are expected to be different from those in temperate zone countries. The aim of this review is to investigate if allergic diseases in the Tropics have particularities that deserve special attention for research and clinical practice. Such information will help to form a better understanding of the pathogenesis, diagnosis and management of allergic diseases in the Tropics. As expected, we found particularities in the Tropics that merit further study because they strongly affect the natural history of common allergic diseases; most of them related to climate conditions that favor permanent exposure to mite allergens, helminth infections and stinging insects. In addition, we detected several unmet needs in important areas which should be investigated and solved by collaborative efforts led by the emergent research groups on allergy from tropical countries.Electronic supplementary materialThe online version of this article (doi:10.1186/s40413-016-0110-7) contains supplementary material, which is available to authorized users.

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A dot-blot and immuno-blot assay for the detection of mosquito saliva specific IgE in mosquito bite allergic subjects

Cited by 3 publications

References 9 publications

Papular urticaria: A review of causal agents in Colombia

Papular urticaria: A review of causal agents in Colombia

Detection of immunoglobulin E using an aptamer based dot-blot assay

Particularities of allergy in the Tropics

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