Analysis of highly expressed genes in the late zygotene to pachytene stages of meiotic prophase I in david lily

Abstract

Plant meiotic prophase I is a complex process involving the late zygotene and pachytene stages, crucial for both completing synapsis and recombination. Using David lily (Lilium davidii var. Willmottiae) as research material, we performed suppressive subtractive hybridization to construct expessed sequence tag (EST) library of anthers at various stages of development by the pollen mother cells. From this library, we identified 34 genes with significantly enhanced expression during the late zygotene to pachytene stages. The cDNA fragment sequences were compared with data in GenBank by BLASTN and BLASTX, and 18 unique ESTs were shown to exhibit significant homology to the data in GenBank. They were classified into eight dif ferent groups: metabolism, protein modification, signal transduction, etc. Through the study of classification and functions of these highly expressed genes during the late zygotene to pachytene stages, we obtained much information about the complex biological progress of meiotic prophase I, especially during chromosome syn apsis and recombination.Abbreviations: DSBs-double strand breaks; EST-expressed sequence tag; NAC-nascent polypeptide associated complex; NOD-no distributive segregation; SSH-suppressive subtrac tive hybridization.

Overexpression of Small Heat Shock Protein LimHSP16.45 in Arabidopsis Enhances Tolerance to Abiotic Stresses

“…Our previous study showed that LimHSP16.45 protects pollen mother cells and tapetal cells against extreme temperatures during the late zygotene to pachytene stages of meiotic prophase I [16], [28]. Therefore, to investigate the relationship between expression and stress, we performed qRT-PCR with anther tissues from David Lily during those developmental stages.…”

“…LimHSP16.45 shares very high identity with other plant cytoplasmic II smHSP, including those from Arabidopsis , Oryza sativa , Pisum sativum , and Vitis vinifera [16]. Those earlier studies demonstrated that LimHSP16.45 can act as a protein chaperone, protecting pollen mother cells and tapetal cells against extreme temperatures [16], [28]. To examine further its functioning and molecular mechanism under abiotic stresses, we transformed LimHSP16.45-GFP into Arabidopsis as our model system.…”