Analysis of growth and lipid production characteristics of <i>Chlorella vulgaris</i> in artificially constructed consortia with symbiotic bacteria

Xue, Lingui; Shang, Hai; Ma, Ping; Wang, X.; He, Xiaoyan; Niu, Junbo; Wu, Juan-Li

doi:10.1002/jobm.201700594

Cited by 23 publications

(6 citation statements)

References 54 publications

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“…Although we cannot rule out the possibility that microalga essentially require some common products of environmental bacteria, wastewater effluent can thus be a valuable resource, not only of nutrients [ 1 – 3 ], but also of MGPB to enhance microalgal biomass productivity. Several reports are available on not only the growth but also the change in cellular composition and flocculation of microalga when co-cultured with bacteria [ 20 , 30 , 39 – 41 ]. In this study, lipid productivity was not considered and this still remaining as a future task.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, lipid productivity was not considered and this still remaining as a future task. However, it may be worth to note that in most cases the content of lipids [ 20 , 39 , 41 ] and hydrocarbons [ 30 ] in microalgae increases by their associated bacteria. On the other hand, long-term effects of indigenous bacterial communities in effluents on microalgal growth must be examined in future studies because some bacteria can initially promote microalgal growth but eventually kill their microalgal host [ 24 – 27 ].…”

Section: Resultsmentioning

confidence: 99%

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Growth promotion of three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris and Euglena gracilis, by in situ indigenous bacteria in wastewater effluent

Toyama

Kasuya

Hanaoka

et al. 2018

Biotechnol Biofuels

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BackgroundMicroalgae are a promising biomass feedstock for biofuels production. The use of wastewater effluent as a nutrient medium would improve the economics of microalgal biofuels production. Bacterial communities in aquatic environments may either stimulate or inhibit microalgal growth. Microalgal productivity could be enhanced if the positive effects of indigenous bacteria could be exploited. However, much is unknown about the effects of indigenous bacteria on microalgal growth and the characteristics of bacterial communities associated with microalgae in microalgae–effluent culture. To assess the effects of the indigenous bacteria in wastewater effluent on microalgal growth, three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris, and Euglena gracilis, were cultured in two municipal wastewater effluents and one swine wastewater effluent with and without indigenous bacteria for 7 days.ResultsAll microalgae grew better in all effluents with indigenous bacteria than without bacteria. Biomass production of C. reinhardtii, C. vulgaris, and E. gracilis increased > 1.5, 1.8–2.8, and > 2.1-fold, respectively, compared to the axenic cultures of each microalga. The in situ indigenous bacterial communities in the effluents therefore promoted the growth of the three microalgae during 7-day cultures. Furthermore, the total numbers of bacterial 16S rRNA genes in the 7-day microalgae–effluent cultures were 109‒793 times the initial numbers. These results suggest that the three microalgae produced and supplied organic carbon that supported bacterial growth in the effluent. At the phylum and class levels, Proteobacteria (Alphaproteobacteria and Betaproteobacteria) and Bacteroidetes (Sphingobacteriia and Saprospirae) were selectively enriched in all microalgae–effluent cultures. The enriched core bacterial families and genera were functions of the microalgal species and effluents. These results suggest that certain members of the bacterial community promote the growth of their “host” microalgal species.ConclusionTo enhance their own growth, microalgae may be able to selectively stimulate specific bacterial groups from among the in situ indigenous bacterial community found in wastewater effluent (i.e., microalgae growth-promoting bacteria: MGPB). The MGPB from effluent cultures could be used as “probiotics” to enhance microalgal growth in effluent culture. Wastewater effluent may therefore be a valuable resource, not only of nutrients, but also of MGPB to enable more efficient microalgal biomass production.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1174-0) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Growth promotion of three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris and Euglena gracilis, by in situ indigenous bacteria in wastewater effluent

Toyama

Kasuya

Hanaoka

et al. 2018

Biotechnol Biofuels

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“…Some MGPB can increase the content of lipids [24, 45–47], fatty acids [47], starch [48], or hydrocarbons [32] in microalgal cells. A. brasilense Cd can significantly increase the accumulation of fatty acids and lipids in C. vulgaris [45, 47]. In this study, however, strain EG3 did not have any positive effect on the accumulation of lipids in E. gracilis (Fig.…”

Section: Discussionmentioning

confidence: 99%

Enhanced production of biomass and lipids by Euglena gracilis via co-culturing with a microalga growth-promoting bacterium, Emticicia sp. EG3

Toyama

Hanaoka

Yamada³

et al. 2019

Biotechnol Biofuels

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BackgroundEuglena gracilis, a unicellular flagellated microalga, is regarded as one of the most promising species as microalgal feedstock for biofuels. Its lipids (mainly wax esters) are suitable for biodiesel and jet fuel. Culture of E. gracilis using wastewater effluent will improve the economics of E. gracilis biofuel production. Enhancement of the productivity of E. gracilis biomass is critical to creating a highly efficient biofuels production system. Certain bacteria have been found to promote microalgal growth by creating a favorable microenvironment. These bacteria have been characterized as microalgae growth-promoting bacteria (MGPB). Co-culture of microalgae with MGPB might offer an effective strategy to enhance microalgal biomass production in wastewater effluent culture systems. However, no MGPB has been identified to enhance the growth of E. gracilis. The objectives of this study were, therefore, to isolate and characterize the MGPB effective for E. gracilis and to demonstrate that the isolated MGPB indeed enhances the production of biomass and lipids by E. gracilis in wastewater effluent culture system.ResultsA bacterium, Emticicia sp. EG3, which is capable of promoting the growth of microalga E. gracilis, was isolated from an E. gracilis-municipal wastewater effluent culture. Biomass production rate of E. gracilis was enhanced 3.5-fold and 3.1-fold by EG3 in the co-culture system using a medium of heat-sterilized and non-sterilized wastewater effluent, respectively, compared to growth in the same effluent culture but without EG3. Two-step culture system was examined as follows: E. gracilis was cultured with or without EG3 in wastewater effluent in the first step and was further grown in wastewater effluent in the second step. Production yields of biomass and lipids by E. gracilis were enhanced 3.2-fold and 2.9-fold, respectively, in the second step of the system in which E. gracilis was co-cultured with EG3 in the first step.ConclusionEmticicia sp. EG3 is the first MGPB for E. gracilis. Growth-promoting bacteria such as EG3 will be promising agents for enhancing E. gracilis biomass/biofuel productivities.

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“…Interesting results were achieved in terms of lipid production with the co-cultivation of Characium sp. And the heterotrophic bacterium, Pseudomonas composti , or with Chlorella vulgaris and Stenotrophomona smaltophilia [ 69 , 70 ]. Parasitism occurs when an organism steals a resource from another organism.…”

Section: Overview On Biological Contaminants In Microalgal Cultivatio...mentioning

confidence: 99%

Fungal Contamination in Microalgal Cultivation: Biological and Biotechnological Aspects of Fungi-Microalgae Interaction

Laezza

Salbitani

Carfagna

2022

JoF

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In the last few decades, the increasing interest in microalgae as sources of new biomolecules and environmental remediators stimulated scientists’ investigations and industrial applications. Nowadays, microalgae are exploited in different fields such as cosmeceuticals, nutraceuticals and as human and animal food supplements. Microalgae can be grown using various cultivation systems depending on their final application. One of the main problems in microalgae cultivations is the possible presence of biological contaminants. Fungi, among the main contaminants in microalgal cultures, are able to influence the production and quality of biomass significantly. Here, we describe fungal contamination considering both shortcomings and benefits of fungi-microalgae interactions, highlighting the biological aspects of this interaction and the possible biotechnological applications.

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Analysis of growth and lipid production characteristics of Chlorella vulgaris in artificially constructed consortia with symbiotic bacteria

Cited by 23 publications

References 54 publications

Growth promotion of three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris and Euglena gracilis, by in situ indigenous bacteria in wastewater effluent

Growth promotion of three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris and Euglena gracilis, by in situ indigenous bacteria in wastewater effluent

Enhanced production of biomass and lipids by Euglena gracilis via co-culturing with a microalga growth-promoting bacterium, Emticicia sp. EG3

Fungal Contamination in Microalgal Cultivation: Biological and Biotechnological Aspects of Fungi-Microalgae Interaction

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