Analysis of genetic variability in two diploid Musa cultivars using RAPD

Abstract

Twenty accessions of the sparsely cultivated diploid Musa cultivars Matti (AA) and Rasakadali (AB) were subjected to random amplified polymorphic DNA (RAPD) assay. A total of 14 random primers were used for the estimation of interand intracultivar variations. Out of 86 bands generated, 64 were polymorphic (74.4 % polymorphism). The cluster analysis grouped the cultivars into two major clusters: cluster I with 10 accessions of Matti and 2 of Rasakadali and cluster II comprising the remaining 8 accessions of Rasakadali. The coefficient of genetic similarity (GS) was from 0.73 to 0.99, suggesting low level of intercultivar variation. The accessions of Rasakadali with mean GS of 0.89 were genetically more diverse than those of Matti (GS = 0.93).Additional key words: banana, genetic similarity coefficient, UPGMA phenogram.

Randomly amplified polymorphic-DNA analysis for detecting genotoxic effects of Boron on maize (Zea mays L.)

“…RAPD-PCR-based assays are important as a genome-wide DNA variation screening strategy (Padmesh et al, 2009). Toxicant-induced genotoxic effects, DNA variation, DNA damage, genetic instability, and mutagenic effects have been evaluated successfully with RAPD analysis.…”

Analysis of Genetic Diversity in <i>Poeciloneuron pauciflorum</i> Bedd.―An Endemic Tree Species from the Western Ghats of India

“…Few fragmented populations distributed in a small geographical region entails an urgent reassessment of the threat category of this species for which we have employed RAPD as tool for estimation of genetic variation in samples collected from few locations across the region. RAPD as a cost effective DNA marker system has been successfully employed to detect varia-tion at the inter-specific [17][18][19], intraspecific [20], intervarietal [21], intergeneric genome analysis [22] and at the cultivar levels [23].…”