Analysis of genetic structure and interrelationships in lentil species using morphological and SSR markers

Abstract

Genetic structure and relationships of 130 lentil accessions belonging to six taxa were analysed. For this purpose, seven morphological traits and 31 polymorphic simple sequence repeat (SSR) primers were used for this purpose. Morphological traits grouped lentil accessions into five main clusters. SSR primers collectively amplified 139 polymorphic alleles in a range of 2-10 with an average of 4.48 alleles. The size of amplified alleles varied from 50 to 650 bp. Polymorphism information content (PIC) ranged from 0.02 to 0.85 with an average of 0.46. Neighbour-joining tree grouped accessions broadly according to their taxonomic ranks, except L. culinaris ssp. odemensis. Analysis of molecular variance (AMOVA) revealed that a major portion (82.0%) of genetic variance resided within species, while only 18% resided among species. Bayesian model-based STRUCTURE analysis assigned all accessions into five clusters and showed some admixture within individuals. Cluster analysis showed that cultivated Lens accessions of Ethiopian origin clustered separately, from other cultivated accessions indicating its distinct lineage. Among the analysed lentil species, L. culinaris ssp. odemensis seemed to have conserved genetic background and needs revision of its taxonomic status. Results of present study provide important information on genetic diversity and relationships among different wild and cultivated taxa of lentil. Thus, these results can be useful in designing breeding strategies for future improvement and taxonomic implications in lentil.

Study of Genetic Diversity on Yield and Yield Attributing Components in Lentil (Lens culinaris Medik.) Under Normal and Late Sown Conditions

“…This tendency of genotypes to occur in clusters cutting across geographical boundaries demonstrates that geographical isolation is not the only factor causing genetic diversity but also show some degree of ancestral relationship. Similar findings were also reported by Sharma et al [3], Koul et al [4]. The genetic divergence is an outcome of several factors such as genetic drift, changing of breeding material, natural variation and artificial selection other than geographical and ecological diversification.…”

The History of Lentil (Lens culinaris subsp. culinaris) Domestication and Spread as Revealed by Genotyping-by-Sequencing of Wild and Landrace Accessions

“…For questions pertaining to population structure and phylogenetic relationships, filtering conditions did not affect the results. Population structure analyses and phylogenies inferred were similar to previous studies: four or five groups, when different species within Lens are considered (depending on whether orientalis and culinaris are split or not) (Alo et al, 2011;Ogutcen et al, 2018;Dissanayake et al, 2020); and three major clusters within cultivated lentils (Khazaei et al, 2016;Koul et al, 2017).…”

“…Two broad varietal types of lentils are recognized based on morphological traits: the large-seeded macrosperma and the small-seeded microsperma ; with a wide diversity of seed color and nutrient content ( Singh et al, 2014 ). The taxonomy of the genus Lens has long been a matter of debate, with genetic, biochemical, morphological, plastid, and hybridization data providing conflicting results regarding its classification at the species and subspecies levels ( Van Oss et al, 1997 ; Ferguson et al, 2000 ; Fratini and Ruiz, 2006 ; Suvorova, 2014 ; Koul et al, 2017 ). The more widely accepted taxonomy recognizes four species: L. culinaris with four subspecies (subsp.…”

“…odemensis , and subsp. tomentosus ), Lens lamottei, Lens ervoides , and Lens nigricans ( Wong et al, 2015 ; Koul et al, 2017 ).…”

Association mapping unveils favorable alleles for grain iron and zinc concentrations in lentil (Lens culinaris subsp. culinaris)

“…[ 109 ] and Koul et al . [ 110 ] reported five subgroups in the lentil germplasm. The genotyping of available genetic diversity has demonstrated the need for incorporation of the exotic germplasm into breeding programs for broadening the genetic base.…”