Analysis of genetic relationship between ‘Bakraee’ (Citrus sp.) and some known Citrus genotypes through SSR and PCR-RFLP markers

Golein, Behrouz; Bigonah, Mojtaba; Azadvar, Mehdi; Golmohammadi, M.

doi:10.1016/j.scienta.2012.10.012

Cited by 19 publications

(12 citation statements)

References 28 publications

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“…Volkameriana was grouped together with Citrus limon 'Eureka' and Citrus limon 'Meyer' and this was in agreement with the findings of Uzun et al (2009). Similarly, Tripolitsiotis et al (2013) observed that Volkameriana presents genetic similarities with various lemon varieties and several researchers (Golein et al, 2012;Hamza, 2013) place Volkameriana in the same genetic group with different varieties of Lime.…”

Section: Genetic Variability Of the Rootstocks Studiedsupporting

confidence: 81%

In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

Salis

Papadakis

Kintzios

et al. 2017

Not Bot Horti Agrobo

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The behavior of six citrus rootstocks, Volkameriana, Citrumelo 'Swingle', Citrange 'Carrizo', Poncirus trifoliata 'Serra', Poncirus trifoliata 'Rubidoux' and Poncirus trifoliata 'Flying Dragon', in in vitro propagation was studied and compared for shoot proliferation and rooting. In addition, the genetic relationships among the rootstocks studied and other Citrus species, using the Inter-Simple Sequence Repeats (ISSR) molecular markers, were investigated. Nodal explants of three months old shoots were used in Murashige and Skoog medium supplemented with N6-benzyladenine (BA) for shoot proliferation and with naphthaleneacetic acid (NAA) for rooting. The rootstock Volkameriana showed a statistically significant higher number of shoots (1.81), shoot length (15.14 mm) and number of leaves per explant (5.81), while all three Poncirus trifoliata rootstocks showed the lowest numbers. The number of roots and root length per explant were evaluated at the end of the rooting phase. The rootstock 'Swingle' showed a higher number of roots per explant (4.2) followed by 'Flying Dragon' (3.93) and 'Carrizo' (3.23) rootstocks. The rootstocks 'Swingle' (140.8 mm), Volkameriana (148 mm) and 'Flying Dragon' (131.12 mm) had significantly higher root length per explant compared to 'Carrizo' (31 mm) and 'Rubidoux' (34.5 mm). The ISSR molecular marker technique used in the present study grouped successfully the different species, varieties and rootstocks studied, revealing their genetic variability. The genetic variability observed among the rootstocks ranged between 0.29 (Poncirus trifoliata 'Serra' and Citrumelo 'Swingle') and 0.60 (Volkameriana and Citrumelo 'Swingle'). The response of the rootstocks studied in in vitro propagation however is not related to their genetic affinity.

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Section: Genetic Variability Of the Rootstocks Studiedsupporting

confidence: 81%

In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

Salis

Papadakis

Kintzios

et al. 2017

Not Bot Horti Agrobo

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“…Its fruits are consumed occasionally despite their bitterness (Shahsavar et al 2007). Recently through SSR and PCR-RFLP markers, Golein et al (2012) found that 'Bakraii' is a hybrid between rough lemon (C. jambhiri Lush. ), as maternal parent, and sweet lime (C. limettioides Tan.).…”

Section: Ssr Unique Allelesmentioning

confidence: 99%

Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers

Sharafi

Abkenar

Sharafi

et al. 2016

Physiol Mol Biol Plants

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Iran has a long history of acid lime cultivation and propagation. In this study, genetic variation in 28 acid lime accessions from five regions of south of Iran, and their relatedness with other 19 citrus cultivars were analyzed using Simple Sequence Repeat (SSR) and Inter-Simple Sequence Repeat (ISSR) molecular markers. Nine primers for SSR and nine ISSR primers were used for allele scoring. In total, 49 SSR and 131 ISSR polymorphic alleles were detected. Cluster analysis of SSR and ISSR data showed that most of the acid lime accessions (19 genotypes) have hybrid origin and genetically distance with nucellar of Mexican lime (9 genotypes). As nucellar of Mexican lime are susceptible to phytoplasma, these acid lime genotypes can be used to evaluate their tolerance against biotic constricts like lime Bwitches' broom disease^.

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“…RAPD (Wu et al 2003), SSR (including nuclear SSR and chloroplast SSR) (Gong et al 2008) were employed to assess the genetic diversity and reveal the phylogenic relationships of Poncirus with other relatives of citrus. SSR, the second generation of molecular marker based on DNA polymerase chain reaction, is currently still and will be a valuable and efficient molecular marker, since more and more ESTs and genomic sequences becoming available (Golein et al 2012;Liu et al 2013). Recently, the high-throughput single nucleotide polymorphisms (SNPs), the third generation of molecular markers has been mined ) and applied in germplasm genotyping (Wu et al 2012) and hybrid verification (Zhu et al 2013c).…”

Section: Discussionmentioning

confidence: 99%

“…Many techniques have been successfully used in citrus phylogenetic relationship analysis, such as AFLP (Amplified fragment length polymorphism) (Pang et al 2007), EST-SSR (expressed sequence tag-simple sequence repeat) and cpSSR (chloroplast SSR) (Wang and Jiang 2010), PCR-RFLP (Polymerase chain reaction-restriction fragment length polymorphism) (Golein et al 2012), RAPD (Randomly amplified polymorphic DNA) (Maya et al 2012). In trifoliate orange, Fang et al (1997) constructed a fingerprint map from 48 vegetatively propagated Poncirus accessions using isozymes, RFLPs and inter-simple sequence repeat markers.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Poncirus and phylogenetic relationships with its relatives revealed by SSR and SNP/InDel markers

et al. 2015

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The polymorphic markers of nuclear SSR (simple sequence repeat), SNP (single nucleotide polymorphism)/InDel (insertion-deletion length polymorphism), and cpSSR (chloroplast SSR) were used to evaluate the genetic diversity of 52 trifoliate oranges and their relatives. The two genomic DNA-based markers produced high values of PIC (polymorphic information content) at 0.45 for SNP/InDel and 0.32 for nuclear SSR, indicating that there is a large diversity of genetic variation within the trifoliate oranges. Three UPGMA (unweighted pair-group method with arithmetic means) dendrograms were generated based on the results of three marker systems. At 0.76 of coefficient, all of Poncirus accessions were clustered into one group, except the 74-1 Early Poncirus, one of the three precocious trifoliate oranges, which was placed separately into another group by nuclear SSR. At a higher coefficient, the trifoliate oranges were discriminated into 4, 6 and 6 groups by nuclear SSR (0.86), SNP/InDel (0.95), and cpSSR (0.95), respectively. The results revealed that three precocious trifoliate oranges were originated from different sources. Hubei Early Poncirus and Beibei Early Poncirus were the asexual mutants of different trifoliate oranges, and 74-1 was probably a sexual hybrid. The present study also indicated that 'Zhiwenzhou,' a diploid chimera of trifoliate orange and Satsuma is more closely related to Satsuma rather than to trifoliate orange, which is in concordance with the results of morphological observation.

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Analysis of genetic relationship between ‘Bakraee’ (Citrus sp.) and some known Citrus genotypes through SSR and PCR-RFLP markers

Cited by 19 publications

References 28 publications

In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers

Genetic diversity of Poncirus and phylogenetic relationships with its relatives revealed by SSR and SNP/InDel markers

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