Analysis of genetic diversity of Indian mango cultivars using RAPD markers

Karihaloo, J. L.; Dwivedi, Yogesh K.; Archak, Sunil; Gaikwad, Ambika B.

doi:10.1080/14620316.2003.11511619

Cited by 79 publications

(43 citation statements)

References 10 publications

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“…The large amplitude (0.07-0.94; average 0.36) of the genetic similarity coefficients established in the present study confirms the occurrence of considerable genetic variability among M. indica cultivars, as previously observed in other RAPD analyses (Karihaloo et al, 2003;Viruel et al, 2005;Rajwana et al, 2008). However, the variation amplitude presently determined was larger than that reported by Karihaloo and co-workers (2003) for Indian mango cultivars (range 0.32-0.75; average 0.56), and by Rajwana and co-workers (2008) for 25 mango cultivars developed in Pakistan (range 0.64-0.89; average 0.74).…”

Section: Discussionsupporting

confidence: 76%

“…There are numerous examples of the application of molecular markers in the analysis of the genetic diversity of mango (Viruel et al, 2005;Pandit et al, 2007;Santos et al, 2008;Singh and Bhat, 2009;Gálvez-López et al, 2009;Singh et al, 2010), several of which have employed the RAPD technique (Karihaloo et al, 2003;Ravishankar et al, 2004;Souza and Lima, 2004;Srivastava et al, 2004;Rajwana et al, 2008;Faleiro et al, 2009). RAPD markers have also been utilized in identifying genitors of open-pollinated mango hybrids generated by the Embrapa Cerrados Plant Improvement Program (Cordeiro et al, 2006a), and in determining the genetic origin of plantlets from polyembryonic mango seeds of cultivar Rosinha (Cor-deiro et al, 2006b).…”

Section: Introductionmentioning

confidence: 99%

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RAPD analysis of the genetic diversity of mango (Mangifera indica) germplasm in Brazil

Souza

Valente²,

Britto³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. We evaluated genetic variability of mango (Mangifera indica) accessions maintained in the Active Germplasm Bank of Embrapa Meio-Norte in Teresina, Piauí, Brazil, using RAPDs. Among these accessions, 35 originated from plantings in Brazil, six from the USA and one from India. Genomic DNA, extracted from leaf material using a commercial purification kit, was subjected to PCR with the primers A01, A09, G03, G10, N05, and M16. Fifty-five polymorphic loci were identified, with mean of 9.16 ± 3.31 bands per primer and 100% polymorphism. Application of unweighted pair group method using arithmetic average cluster analysis demonstrated five genotypic groups among the accessions examined. The genotypes Rosa 41, Rosa 48 and Rosa 49 were highly similar (94% similarity), whereas genotypes Sensation and Rosa 18 were the most divergent (only 7% similarity). The mango accessions were found to have considerable genetic variability, RAPD analysis of the genetic diversity of mango germplasm demonstrating the importance of analyzing each genotype in a collection in order to efficiently maintain the germplasm collection.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

RAPD analysis of the genetic diversity of mango (Mangifera indica) germplasm in Brazil

Souza

Valente²,

Britto³

et al. 2011

Genet. Mol. Res.

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“…DNA-based markers such as RAPD, are practically unlimited in number, remain unaffected by environment and growth conditions and are simply inherited (Karihaloo et al, 2003). Thus, this RAPD analysis provided phylogenetic relationships as well as some unique DNA bands to quantify the genetic variation.…”

Section: Discussionmentioning

confidence: 99%

“…Due to the technical simplicity and speed of RAPD methodology, it has been successfully used for the assessment of genetic structure and phylogenetic analysis (Gepts, 1993). It has been successfully applied to studies of genetic differentiation in some genera-like Mangifera (Karihaloo et al, 2003); Asparagus ; Eucalyptus (Keil and Griffin, 1994) and Gossypium (Multani and Lyon, 1995), etc.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity in Aloe germplasm accessions from India using RAPD and morphological markers

Nayanakantha

Singh²,

Gupta

2010

Ceylon J. Sci. (Biol. Sci.)

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Eleven Aloe germplasm accessions; A. vera, A. perryi, A. lotus, A. zeylanicum and seven strains of A. vera available at the Defense Agricultural Research Laboratory (DARL), Pithoragarh, and Medicinal and Aromatic Plant Research Development Centre (MRDC), Pantnagar, Uttaranchal, India were subjected to Random Amplified Polymorphic DNA (RAPD) analysis in relation to morphometric parameters for estimating the extent of diversity within and between species. Morphological evaluation of the 11 accessions for selected characters showed qualitative variation among the accessions studied. The RAPD analysis revealed comparable inter and intra species variation. A total of 192 bands were amplified with 7 primers. Out of 192 bands amplified, 89% was polymorphic and 10.9% was unique to a particular accession which made it distinct from all other accessions. Maximum similarity of 61% was observed between DARL 1 and DARL 3 (A. vera) and minimum similarity of 6.8 % was observed between A. lotus and A. perryi. Thus, Aloe accessions maintained at DARL showed high genetic diversity. The RAPD profiles would be useful in genetic improvement and authentication of species and genotype of this medicinally and economically important genus.

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“…WIDE SNP MARKERS Molecular diversity analysis and fingerprinting of mango cultivars has been carried out using different types of DNA markers, including random amplified polymorphic DNA (RAPD) by Schnell et al (1995); LopezValenzuela et al (1997); Ravishankar et al (2000); Kumar et al (2001); Karihaloo et al (2003); inter simple sequence repeats (ISSR) by Eiadthong et al 1999;Pandit et al (2007) and Singh et al (2007); amplified fragment length polymorphism (AFLP) by Eiadthong et al (2000); Kashkush et al (2001) and simple sequence repeats (SSR) by Malathi et al (2013). However, most of these studies were carried it on small sets of genotypes.…”

Section: Diversity and Population Structure Of Mango Cultivars Based mentioning

confidence: 99%

Origin, Diversity and Genome Sequence of Mango (Mangifera indica L.)

Singh¹,

Mahato²,

Jayaswal³

et al. 2016

Indian Journal of History of Science

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Mango (Mangifera indica L.) is known as the 'king of fruits' for its rich taste, flavor, color, production volume and diverse end usage. It belongs to plant family Anacardiaceae and has a small genome size of 439 Mb (2n = 40). Ancient literature indicates origin of cultivated mango in India. Although wild species of genus Mangifera are distributed throughout South and South-East Asia, recovery of Paleocene mango leaf fossils near Damalgiri, West Garo Hills, Meghalaya point to the origin of genus in peninsular India before joining of the Indian and Asian continental plates. India produces more than fifty percent of the world's mango and grows more than thousand varieties. Despite its huge economic significance genomic resources for mango are limited and genetics of useful horticultural traits are poorly understood. Here we present a brief account of our recent efforts to generate genomic resources for mango and its use in the analysis of genetic diversity and population structure of mango cultivars. Sequencing of leaf RNA from mango cultivars 'Neelam', 'Dashehari' and their hybrid 'Amrapali' revealed substantially higher level of heterozygosity in 'Amrapali' over its parents and helped develop genic simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. Sequencing of double digested restriction-site-associated genomic DNA (ddRAD) of 84 diverse mango cultivars identified 1.67 million high quality SNPs and two major sub-populations. We have assembled 323 Mb of the highly heterozygous 'Amrapali' genome using long sequence reads of PacBio single molecule real time (SMRT) sequencing chemistry and predicted 43,247 protein coding genes. We identified in the mango genome 122,332 SSR loci and developed 8,451 Type1 SSR and 835 HSSR markers for high level of polymorphism. Among the published genomes, mango showed highest similarity with sweet orange (Citrus sinensis). These genomic resources will fast track the mango varietal improvement for high productivity, disease resistance and superior end use quality.

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Analysis of genetic diversity of Indian mango cultivars using RAPD markers

Cited by 79 publications

References 10 publications

RAPD analysis of the genetic diversity of mango (Mangifera indica) germplasm in Brazil

RAPD analysis of the genetic diversity of mango (Mangifera indica) germplasm in Brazil

Assessment of genetic diversity in Aloe germplasm accessions from India using RAPD and morphological markers

Origin, Diversity and Genome Sequence of Mango (Mangifera indica L.)

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