Assessment of genetic diversity in Aloe germplasm accessions from India using RAPD and morphological markers

Nayanakantha, N. M. C.; Singh, Balraj; Gupta, Atul

doi:10.4038/cjsbs.v39i1.2315

Cited by 14 publications

(11 citation statements)

References 26 publications

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“…In India, [28,41] used RAPD and AFLP markers to assess genetic diversity among Aloe accessions from different geographical regions. Similar results have been reported by [10] and [29] on genetic variability in Aloe vera where both RAPD and ISSR primers have been used. Moreover, in this study, there was genetic diversity in each population as revealed by the Nei's genetic diversity indices (H) which varied between the ISSR and RAPD markers.…”

Section: Population Genetic Parameters: Percentage Of Polymorphic Bsupporting

confidence: 87%

“…Genetic diversity is a key component to biodiversity analysis and therefore it's important to have knowledge of the distribution, genetic diversity, environment and relations among plant varieties to recognize gene pools, identify gaps in germplasm collections and develop effective conservation and management strategies [10]. There are morphological variations in some economically important Aloe species [11].…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and population structure of three commercial indigenous Aloe species in selected ASALs of Kenya

Adienge¹,

Muturi²,

Nadir³

et al. 2019

J Plant Sci Mol Breed

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Aloe species are common in arid and semi-arid lands (ASALs) of Kenya and are of economic importance especially for bitter gum production. However, the species is under ecological threat due to unsustainable harvesting from the wild. The objective of this study was to evaluate genetic diversity and population structure of three commercially exploited indigenous Aloe species; A. secundiflora, A. turkanensis and A. scabrifolia.The study was carried out in fifteen populations of Aloe species in their wild habitat where their geographic distribution was surveyed and populations delineated. Within each population, 30 trees were sampled at a distance of 100 m apart where leaf tissues were collected for DNA isolation and analysis using modified Cetyl trimethyl ammonium bromide (CTAB) method. Randomly amplified polymorphic DNA (RAPDs) and Inter-simple sequence repeats (ISSR) markers were used to determine the genetic diversity and population structure. Ten RAPD and seven ISSR discriminative primers with the highest value of genetic diversity were selected to genotype three indigenous Aloe species. The genetic similarity was performed using Popgene 1.3.1; hierarchical UPGMA cluster analysis and principal coordinate analysis were performed using Genalex 6.5. To map genetic structure and gene pools, both Structure and Structure Harvester statistical packages were used. From the results, A. secundiflora species was the most distributed in the surveyed ASALs. There was high genetic variation among and within the populations. The combined use of ISSR and RAPD revealed high variations among the populations as compared to when either of them used singly. Genetic variation was highest within secundiflora species and least within turkanensis species. Maralal secundiflora population had the highest genetic variation. The study exhibited a population genetic structure with three major clusters which corresponded to the three Aloe species with minimal reproductive crossing among them. It is therefore, recommended to undertake biophysical studies to determine the advantages of A.secundiflora that has over the other species. In addition, Maralal sec population should be conserved ex-situ because of its high genetic diversity while both ISSR and RAPD markers should be prioritized in Aloe genetic studies as they reveal high variation.

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Section: Population Genetic Parameters: Percentage Of Polymorphic Bsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of three commercial indigenous Aloe species in selected ASALs of Kenya

Adienge¹,

Muturi²,

Nadir³

et al. 2019

J Plant Sci Mol Breed

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“…RAPD and AFLP markers are used to analyze the germplasm diversity in Aloe species [26]. A. vera (A 1 ) from different accessions in India showed a good genetic variability based on RAPD analysis [27]. Recently, genetic diversity in A. vera accessions from Iran was also studied based on agro-morphological, phytochemical, and RAPD tests [28].…”

Section: Original Papersmentioning

confidence: 99%

Use of Random Amplified Polymorphic DNA (RAPD) Technique to Study the Genetic Diversity of Eight Aloe Species

2016

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The genus comprises over 400 species of flowering succulent plants. leaves are used in the treatment of asthma, gastrointestinal ulcers, cardiovascular disease, tumors, burns, and diabetes. They are rich in anthraquinones, such as aloin, aloe-emodin, chrysophanol, aloinoside A, and aloinoside B. The various species of show chemical and morphological similarity and diversity, which depend on the genotype and environmental conditions. In a continuity to our interest in the genus, this study targets the authentication of eight different species, (A) (A) (A) (A) (A), (A) (A), and (A), grown in Egypt by using the technique of random amplified polymorphic DNA. Twelve decamer primers were screened in amplification with genomic DNA extracted from all species, of which five primers yielded species-specific reproducible bands. Out of 156 loci detected, the polymorphic, monomorphic, and unique loci were 107, 26, and 23, respectively. Based on a dendrogram and similarity matrix, the eight species were differentiated from each other and showed more divergence. species prevailed similarity coefficients of 54-70 % by which they could be classified into three major groups. Thus, this technique may contribute to the identification of these species that have great morphological similarity in the Egyptian local markets.

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“…Maintaining the species or varieties of aloe using sexual reproduction is difficult because the natural hybridization of this species is very frequent (Granados-Sánchez and Castañeda-Pérez, 1998). Nayanakantha et al (2010a) and Alagukannan and Ganesh (2016) have revealed the existence of substantial genetic diversity among the Indian accessions for yield and quality parameters.…”

Section: Propagation Techniquesmentioning

confidence: 99%

“…The cytokinins, supplemented to the culture medium, overcome apical dominance releasing lateral buds from dormancy (George et al, 2008) and BA and BAP (same compound named differently according to Teixeira da Silva, 2012) are the synthetic cytokinins commonly used. By this way, elite plant material can be efficiently produced with low or no somaclonal variation (Nayanakantha et al, 2010a). The following different steps should be faced to reach a satisfactory protocol to obtain virus-free plants.…”

Section: Propagation Techniquesmentioning

confidence: 99%

Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

2016

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Barbados aloe (Aloe vera (L.) Burm. F.) has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic, and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe, and America. In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems.

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Assessment of genetic diversity in Aloe germplasm accessions from India using RAPD and morphological markers

Cited by 14 publications

References 26 publications

Genetic diversity and population structure of three commercial indigenous Aloe species in selected ASALs of Kenya

Genetic diversity and population structure of three commercial indigenous Aloe species in selected ASALs of Kenya

Use of Random Amplified Polymorphic DNA (RAPD) Technique to Study the Genetic Diversity of Eight Aloe Species

Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

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