Analysis of genetic diversity of Laeliinae (Orchidaceae) in the State of Sergipe using ISSR markers

Abstract

ABSTRACT. The Orchidaceae represent one of the largest and most diverse families on the planet. However, this family is constantly threatened by predators and by the advancement of urban centers over its natural habitats. The objective of this study was to use inter-simple sequence repeat markers to evaluate the genetic diversity between orchid accessions of the Laeliinae subtribe, which comprise part of the Orchidaceae study collection at the Department of Agronomic Engineering of the Federal University of Sergipe. DNA was extracted from each specimen by using an adapted 2% cetyltrimethyl ammonium bromide protocol. Similarity between individuals was calculated using the Jaccard method. Clustering was carried out by the unweighted pair group method with arithmetic mean method, with resampling and 10,000 bootstraps. Eighty-seven fragments were obtained, all of which were polymorphic, revealing high variability between accessions. The mean similarity was 35.77% between Encyclia sp individuals, and 35.90% between specimens of Cattleya tigrina. For Epidendrum secundum, a relationship between geographic and genetic distances was observed, and the accession collected in the southern part of the State of Sergipe (Serra de Itabaiana National Park) was more divergent than that of the other parts of the state. The data generated in this study will guide further research aimed at the ex situ conservation of these materials.

Propuesta de Árbol Temático y Tecnológico sobre la Ingeniería Agroindustrial como Herramienta para un Estudio de Prospectiva

“…Para la producción de los biocombustibles, uno de los principales sustratos empleado es lignocelulosa, ya sea en empleando enzimas o microorganismos, (Kumar et al, 2017;Saini et al, 2015). El otro campo de la biotecnología corresponde a las modificadoras genéticas o las denominadas transgénesis de todo tipo de organismos vivos, tales como microorganismos, plantas y animales; la mayoría de los avances se centran en modificaciones genéticas de plantas tanto ornamentales, para la obtención de materias primas para la industria, o plantas para alimentación animal o humana (Ali et al, 2016;Arrigoni-Blank et al, 2016;Shin et al, 2016). En este tema se destaca no solamente los avances en los mejoramientos de microorganismos, sino la percepción que tienen los diferentes sectores de la sociedad con respecto a la transgénesis (Ahmad et al, 2016).…”

Vegetative development and flowering of Encyclia hybrid based on fertilization and substrate

“…The genus Encyclia presents approximately 120 species, distributed throughout the United States, Mexico, Brazil and Argentina. Brazil is home to 56 species, of which 42 are endemic (Arrigoni-Blank et al, 2015), with a high geographical distribution (Pansarin and Pansarin, 2010).…”

“…The species are generally epiphytic plants, but there are many rupicolous and rare terrestrials (Arrigoni-Blank et al, 2015). Encyclia has long, lanceolate and coriaceous leaves, 30-40 cm erect inflorescence with small fragrant flowers, with varied sepals, petals and lip colors.…”