Analysis of galactoglucomannans from spruce wood by capillary electrophoresis

Abstract

The aim of this study was to setup a method for detection and quantification of monosaccharide components in technical galactoglucomannas (T-GGM) from spruce wood using capillary zone electrophoresis (CZE). CZE technique was optimised regarding borate buffer concentrations, EOF modifier application, and system pH. Aqueous solution of T-GGM was chemically hydrolysed by sulphuric acid, in an autoclave. In this way obtained monosaccharides were derivatized with 4-amino benzoic acid ethyl ester via reductive amination using sodium cyanoborohydride. The results of the optimisation procedure showed that the borate buffers at lowest concentrations (100 and 200 mM) with acetonitrile addition as EOF modifier gave the optimal measurement results, as it showed sufficient separation at relatively short migration times. The amounts of single monosaccharide components in the T-GGM samples obtained by the optimised CZE procedure were practically the same in comparison to the results of the well established HPLC-anion exchange chromatography. On the basis of this research, it was concluded that the capillary zone electrophoresis is an efficient analytical procedure for the characterisation of galactoglucomannans derived from softwoods.

Characterisation of the exopolysaccharide kefiran produced by lactic acid bacteria entrapped within natural kefir grains

“…The derivatisation of hydrolysates was performed through reductive amination, using sodium cyanoborohydride according to Doliška et al. (2009).…”

Characterization of hydrothermally isolated xylan from beech wood by capillary electrophoresis with laser-induced fluorescence and mass spectrometry detection

“…CE showed better performance than HPLC in the separation of low molecular xylo-oligomers. Furthermore, CE-LIF and CE-MS has also been used for the characterization of other hemicelluloses, for example, for arabino-oligosaccharides (Westphal et al 2010) or galactoglucomannans (Doliška et al 2009). The use of CE for monosaccharide and oligosaccharide analysis in the pulp and paper industry has been reviewed (Hiltunen and Sirén 2013).…”