Analysis of Fuzhisan and quantitation of baicalin and ginsenoside Rb1 by HPLC-DAD-ELSD

Zhao, Jingkun; Wang, Desheng; Duan, Shanshan; Wang, Jianxiu; Bai, Jing; Li, Wenlan

doi:10.1007/s12272-009-1703-2

Cited by 21 publications

(31 citation statements)

References 15 publications

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“…Fuzhisan (FZS), a Chinese herbal complex prescription containing Ginseng root (Araliaceae family), Scutellaria baicalensis Georgi (Labiatae family), Anemone altaica (Araceae family) and Glycyrrhiza uralensis (Leguminosae family) [30,31], has been used in the treatment of senile dementia for over 15 years. The chemical fingerprint of 12 batches of FZS has been established to quantify its components and the similarity among 12 batches of FZS was over 0.95 [31].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Fuzhisan, a Chinese Herbal Medicine, Inhibits Beta-Amyloid-Induced Neurotoxicity and Tau Phosphorylation Through Calpain/Cdk5 Pathway in Cultured Cortical Neurons

et al. 2011

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It has been shown that β-amyloid (Aβ) induced hyperphosphorylation of tau is implicated in the pathogenesis of Alzheimer's disease (AD), and deregulation of cyclin-dependent kinase 5 (Cdk5) activity is involved in the abnormal tau phosphorylation. The cleavage of neuron-specific Cdk5 activator, p35, to p25, mediated by calpain and calcium, deregulates Cdk5 activity and promotes neurodegeneration. Fuzhisan (FZS), a Chinese herbal complex prescription that has been used for the treatment of AD for over 15 years, is known to enhance the cognitive ability in AD patients. In this study, we investigated the neuroprotective effects and potential molecular mechanisms of FZS against Aβ(25-35)-induced toxicity in cultured cortical neurons. We revealed that FZS attenuated Aβ(25-35)-induced neurotoxicity in a dose-dependent manner. FZS inhibited Aβ(25-35)-induced activation of Cdk5 and decreased tau hyperphosphorylation although it did not directly inhibit Cdk5. In addition, FZS also blocked Aβ(25-35)-induced calcium influx, calpain activation and decreased cleavage of p35 to p25.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Fuzhisan, a Chinese Herbal Medicine, Inhibits Beta-Amyloid-Induced Neurotoxicity and Tau Phosphorylation Through Calpain/Cdk5 Pathway in Cultured Cortical Neurons

et al. 2011

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“…The voucher specimens were authenticated by Prof. De-Sheng Wang and deposited at the Department of Neurology of Harbin Medical University. The methanol extract of AS was carried out according to Zhao et al (2009) as follows: A 10.0-g sample powder with diameter less than 0.125 mm was extracted with 300 ml methanol at a constant temperature of 758C for 60 min. The extraction process was repeated three times, and all extracted solutions were combined, filtered, and evaporated to dryness.…”

Section: Angelica Sinensis Extract Preparationmentioning

confidence: 99%

Inhibition of glycogen synthase kinase-3β by Angelica sinensis extract decreases β-amyloid-induced neurotoxicity and tau phosphorylation in cultured cortical neurons

Zhang

Zhao

et al. 2010

J. Neurosci. Res.

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Increasing evidence has shown that β-amyloid (Aβ) induces hyperphosphorylation of tau and contributes to Aβ toxicity. Recently, tau hyperphosphorylation by glycogen synthase kinase-3β (GSK-3β) activation has been emphasized as one of the pathogenic mechanisms of Alzheimer's disease (AD). The phosphoinositide 3 kinase (PI3K)/Akt pathway is known as an upstream element of GSK-3β. The inhibitory control of GSK-3β, via the PI3K/Akt pathway, is an important mechanism of cell survival. In the present study, we investigated the neuroprotective effects of Angelica sinensis (AS), a traditional Chinese herbal medicine, against Aβ(1-42) toxicity in cultured cortical neurons and also the potential involvement of PI3K/Akt/GSK-3β signal pathway. We revealed that AS extract significantly attenuated Aβ(1-42) -induced neurotoxicity and tau hyperphosphorylation at multiple AD-related sites in a dose-dependent manner. Simultaneously, it increased the levels of phospho-Ser(473) -Akt and down-regulated GSK-3β activity by PI3K activation. The neuroprotective effects of AS extract against Aβ(1-42) -induced neurotoxicity and tau hyperphosphorylation were blocked by LY294002 (10 μM), a PI3K inhibitor. In addition, AS extract reversed the Aβ(1-42) -induced decrease in phosphorylation cyclic AMP response element binding protein (CREB), which could be blocked by the PI3K inhibitor. These results suggest that AS-mediated neuroprotection against Aβ toxicity is likely mediated by the PI3K/Akt/GSK-3β signal pathway.

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“…In recent years, an on-line coupled HPLC-DAD-ELSD method has attracted ever-increasing attention and has been successfully applied to simultaneous quantification of multi-components in traditional Chinese herbal medicines and their preparations [14][15][16][17][18][19][20][21][22][23]. This method is potentially ideal for routine analysis and quality evaluation of YST since UV and ELSD signals contain complementary information for each other.…”

Section: Introductionmentioning

confidence: 99%

Quality evaluation ofYanghuo Sanqitablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

Zhu¹,

Jia²,

Cai³

et al. 2013

Acta Chromatographica

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Summary. Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography-diode array detector-evaporative light-scattering detector (HPLC-DAD-ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile-water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min −1 . The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC-DAD-ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

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Analysis of Fuzhisan and quantitation of baicalin and ginsenoside Rb1 by HPLC-DAD-ELSD

Cited by 21 publications

References 15 publications

RETRACTED ARTICLE: Fuzhisan, a Chinese Herbal Medicine, Inhibits Beta-Amyloid-Induced Neurotoxicity and Tau Phosphorylation Through Calpain/Cdk5 Pathway in Cultured Cortical Neurons

RETRACTED ARTICLE: Fuzhisan, a Chinese Herbal Medicine, Inhibits Beta-Amyloid-Induced Neurotoxicity and Tau Phosphorylation Through Calpain/Cdk5 Pathway in Cultured Cortical Neurons

Inhibition of glycogen synthase kinase-3β by Angelica sinensis extract decreases β-amyloid-induced neurotoxicity and tau phosphorylation in cultured cortical neurons

Quality evaluation ofYanghuo Sanqitablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

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