Analysis of freeze-gelation and cross-linking processes for preparing porous chitosan scaffolds

Hsieh, Chien-Yang; Tsai, Sung-Pei; Ho, Ming-Hwa; Wang, Da-Ming; Liu, Chung-En; Hsieh, Cheng-Hsuan; Tseng, Hsien-Chung; Hsieh, Hsun-Ping

doi:10.1016/j.carbpol.2006.05.002

Cited by 106 publications

(68 citation statements)

References 23 publications

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“…The solvent is extracted by a non solvent and the remaining space becomes porous, resulting in a polymer membrane. Various pore structures and morphologies can be achieved by varying the cooling rate, adjusting the polymer concentration, and changing the solvent system [20,21]. The technique of freeze gelation has also been reported to offer a more convenient, time and energy-efficient method to fabricating porous membranes compared with freeze-drying and offers an easy to scale up process [21].…”

Section: Introductionmentioning

confidence: 99%

Freeze gelated porous membranes for periodontal tissue regeneration

et al. 2015

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Guided tissue regeneration (GTR) membranes have been used for the management of destructive forms of periodontal disease as a means of aiding regeneration of lost supporting tissues, including the alveolar bone, cementum, gingiva and periodontal ligaments (PDL). Currently available GTR membranes are either non-biodegradable, requiring a second surgery for removal, or biodegradable. The mechanical and biofunctional limitations of currently available membranes result in a limited and unpredictable treatment outcome in terms of periodontal tissue regeneration. In this study, porous membranes of chitosan (CH) were fabricated with or without hydroxyapatite (HA) using the simple technique of freeze gelation (FG) via two different solvents systems, acetic acid (ACa) or ascorbic acid (ASa). The aim was to prepare porous membranes to be used for GTR to improve periodontal regeneration. FG membranes were characterized for ultra-structural morphology, physiochemical properties, water uptake, degradation, mechanical properties, and biocompatibility with mature and progenitor osteogenic cells. Fourier transform infrared (FTIR) spectroscopy confirmed the presence of hydroxyapatite and its interaction with chitosan. μCT analysis showed membranes had 85-77% porosity. Mechanical properties and degradation rate were affected by solvent type and the presence of hydroxyapatite. Culture of human osteosarcoma cells (MG63) and human embryonic stem cell-derived mesenchymal progenitors (hES-MPs) showed that all membranes supported cell proliferation and long term matrix deposition was supported by HA incorporated membranes. These CH and HA composite membranes show their potential use for GTR applications in periodontal lesions and in addition FG membranes could be further tuned to achieve characteristics desirable of a GTR membrane for periodontal regeneration.

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Section: Introductionmentioning

confidence: 99%

Freeze gelated porous membranes for periodontal tissue regeneration

et al. 2015

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“…Chitosan is polysaccharide that degrades in the presence of naturally occurring enzymes such as lysozyme [5][6][7]. The low mechanical strength of chitosan hydrogel can be improved by using a crosslinker such as glutaraldehyde (GA) [8,9], genipin (GP) [10], 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) [11] or tripolyphosphate [12].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of porous chitosan scaffolds for soft tissue engineering using dense gas CO2

et al. 2011

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“…17 Briefly speaking, the chitosan/acetic acid solution was sprayed into NaOH-glycerol coagulant bath at 220°C and immersed TPP solution at 4°C for 2 h. The gelation of chitosan resulted in chitosan microspheres. After dried at room temperature, the chitosan particles were showed with the diameter ranged from 60 to 120 lm.…”

Section: Preparation and Characterization Of Chitosanmentioning

confidence: 99%

“…17 Chitosan powder was dissolved in an acetic acid aqueous solution (1 M) to form a 2 wt.% polymer solution. The solution was used to prepare chitosan membranes.…”

Section: Preparation and Characterization Of Chitosanmentioning

confidence: 99%

Preparation of Chitosan/Hydroxyapatite Substrates with Controllable Osteoconductivity Tracked by AFM

Hsiao

et al. 2014

Ann Biomed Eng

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In this study, cell-material adhesive strength and cellular mechanical properties were measured using atomic force microscopy (AFM) to track cell attachment and osteogenic differentiation. First, chitosan substrates were treated with simulated body fluid (SBF) for various periods, resulting in substrates with different osteoconductivity. The X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS) and in vitro tests revealed that the biomimeticity and osteoconductivity of substrates increased with increasing time of SBF treatment. When the SBF immersion exceeded 14 days, the chitosan substrates exhibited their highest biocompatibility and osteoconductivity. AFM measurements indicated specifically high adhesive forces between SBF-treated chitosan and osteogenic cells, causing better cell attachment. The results demonstrate that cell adhesion was controlled by cell-material adhesive strength, which were in turn controlled via the SBF treatment time. The adhesive strength between cells and material also accounted for the chitosan substrates' specific selectivity toward osteogenic cells. A two-step increase in mechanical strength was observed for the nucleus and cytoplasm of osteogenic cells. The results indicate that through the use of AFM, the real-time cell-material interforce and cellular mechanics can be identified. The adhesive strength was positively correlated to the cell attachment, and the second increase in the Young's modulus of nucleus and cytoplasm was correlated to early osteogenic differentiation.

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Analysis of freeze-gelation and cross-linking processes for preparing porous chitosan scaffolds

Cited by 106 publications

References 23 publications

Freeze gelated porous membranes for periodontal tissue regeneration

Freeze gelated porous membranes for periodontal tissue regeneration

Fabrication of porous chitosan scaffolds for soft tissue engineering using dense gas CO2

Preparation of Chitosan/Hydroxyapatite Substrates with Controllable Osteoconductivity Tracked by AFM

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