Analysis of four parabens and bisphenols A, F, S in urine, using dilute and shoot and liquid chromatography coupled to mass spectrometry

Sanchís, Yovana; Coscollà, Clara; Yusà, Vicent

doi:10.1016/j.talanta.2019.04.048

Cited by 29 publications

(7 citation statements)

References 21 publications

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“…The urinary biomarkers of bisphenols, parabens and phthalates determined in the present study are shown in Table S2. The determination of bisphenols and parabens in urine was implemented following the method described in Sanchis et al [31]. Firstly, the enzymatic hydrolysis was implemented by adding 200 µL of 1M ammonium acetate buffer (pH 5.0) and 10 µL of β-glucuronidase/arylsulfatase to 500 µL of sample.…”

Section: Determination Of Urinary Biomarkersmentioning

confidence: 99%

Biomonitoring of Phthalates, Bisphenols and Parabens in Children: Exposure, Predictors and Risk Assessment

Dualde

León²,

Sanchís³

et al. 2021

IJERPH

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Exposure to emerging contaminants, such as phthalates, bisphenols and parabens in children has been associated with possible neurodevelopment and endocrine alterations. In the present study, the biomonitoring of biomarkers in children (5–12 years old) from the Valencia Region (Spain) have been implemented using urines from the BIOVAL program. More than 75% of the children studied (n = 562) were internally exposed (>LOQ) to bisphenols and parabens, and the whole population assessed (n = 557) were exposed to at least one phthalate. The geometric means (GM) of the concentrations of bisphenol A, methyl paraben and propyl paraben were 0.9, 1.4 and 0.39 ng/mL, respectively. Regarding phthalates, monoethyl phthalate GM was 55.0 ng/mL and diethyl hexyl phthalate (as the sum of five metabolites) GM was 60.6 ng/mL. Despite the studied population being widely exposed, the detection frequencies and concentrations were in general lower than in previous studies involving children in Spain and in other countries in recent years. Furthermore, the risk assessment study concluded that the internal exposure to phthalates, bisphenols and parabens is lower than the guidance values established, and, therefore, a health risk derived from the exposure to these compounds in the studied population is not expected.

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Section: Determination Of Urinary Biomarkersmentioning

confidence: 99%

Biomonitoring of Phthalates, Bisphenols and Parabens in Children: Exposure, Predictors and Risk Assessment

Dualde

León²,

Sanchís³

et al. 2021

IJERPH

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“…The determination of PBs, BPA and its analogs, TCS and BP-3, in human urine have been analyzed by sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) followed by various sample pretreatment approaches [30][31][32][33][34]. However, the elimination of the matrix effect during ionization, an unavoidable phenomenon, using any set of sample pretreatment protocols or chromatographic systems is complex and may cause bias in quantitative data [35,36].…”

Section: Introductionmentioning

confidence: 99%

Determination of Parabens, Bisphenol A and Its Analogs, Triclosan, and Benzophenone-3 Levels in Human Urine by Isotope-Dilution-UPLC-MS/MS Method Followed by Supported Liquid Extraction

Chen

Chang

Sun

et al. 2022

Toxics

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The development of a rapid analytical approach for determining levels of antibacterial agents, plasticizers, and ultraviolet filters in biosamples is crucial for individual exposure assessment. We developed an analytical method to determine the levels of four parabens—bisphenols A (BPA) and its analogs, triclosan (TCS), triclocarban, and benzophenone-3 (BP-3)—in human urine. We further measured the levels of these chemicals in children and adolescents. We used a supported liquid extraction (SLE) technique coupled with an isotope-dilution ultraperformance liquid chromatography-tandem mass spectrometry (ID-UPLC-MS/MS) method to assess the detection performance for these chemicals. Forty-one urine samples from 13 children and 28 adolescents were assessed to demonstrate the capability and feasibility of our method. An acceptable recovery (75.6–102.4%) and matrix effect (precision < 14.2%) in the three-level spiked artificial urine samples were achieved, and good performance of the validated ID-UPLC-MS/MS method regarding linearity, limits of detection, and quantitation was achieved. The within-run and between-run accuracy and precision also demonstrated the sensitivity and stability of this analytical method, applied after SLE. We concluded that the ID-UPLC-MS/MS method with SLE pretreatment is a valuable analytical method for the investigation of urinary antibacterial agents, plasticizers, and ultraviolet filters in humans, useful for human biomonitoring.

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“…As for the analysis, liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) is the most used technique to analyse organic compounds in urine [30,31]. Low-resolution LC-MS/MS is used for target analysis and quantification of EDCs due to its low detection limits and robust performance [14,32]. Within that targeted analysis context, most studies in the literature are focused on a reduced number of EDC families with similar chemical properties, instead of developing methods to simultaneously determine a wide variety of compounds with different chemical behaviours.…”

Section: Introductionmentioning

confidence: 99%

From target analysis to suspect and non-target screening of endocrine-disrupting compounds in human urine

et al. 2022

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In the present work, a target analysis method for simultaneously determining 24 diverse endocrine-disrupting compounds (EDCs) in urine (benzophenones, bisphenols, parabens, phthalates and antibacterials) was developed. The target analysis approach (including enzymatic hydrolysis, clean-up by solid-phase extraction and analysis by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS)) was optimized, validated and applied to volunteers’ samples, in which 67% of the target EDCs were quantified. For instance, benzophenone-3 (0.2–13 ng g−1), bisphenol A (7.7–13.7 ng g−1), methyl 3,5-dihydroxybenzoate (8–254 ng g−1), mono butyl phthalate (2–17 ng g−1) and triclosan (0.3–9 ng g−1) were found at the highest concentrations, but the presence of other analogues was detected as well. The developed target method was further extended to suspect and non-target screening (SNTS) by means of LC coupled to high-resolution MS/MS. First, well-defined workflows for SNTS were validated by applying the previously developed method to an extended list of compounds (83), and then, to the same real urine samples. From a list of approximately 4000 suspects, 33 were annotated at levels from 1 to 3, with food additives/ingredients and personal care products being the most abundant ones. In the non-target approach, the search was limited to molecules containing S, Cl and/or Br atoms, annotating 4 pharmaceuticals. The results from this study showed that the combination of the lower limits of detection of MS/MS and the identification power of high-resolution MS/MS is still compulsory for a more accurate definition of human exposome in urine samples. Graphical abstract

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Analysis of four parabens and bisphenols A, F, S in urine, using dilute and shoot and liquid chromatography coupled to mass spectrometry

Cited by 29 publications

References 21 publications

Biomonitoring of Phthalates, Bisphenols and Parabens in Children: Exposure, Predictors and Risk Assessment

Biomonitoring of Phthalates, Bisphenols and Parabens in Children: Exposure, Predictors and Risk Assessment

Determination of Parabens, Bisphenol A and Its Analogs, Triclosan, and Benzophenone-3 Levels in Human Urine by Isotope-Dilution-UPLC-MS/MS Method Followed by Supported Liquid Extraction

From target analysis to suspect and non-target screening of endocrine-disrupting compounds in human urine

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