Revista:FOOD CHEMISTRY, nº 160, p. 313-320
ABSTRACTVeterinarian and human pharmaceuticals may be intentionally added to animal feed to enhance animal production. Monitoring these substances is necessary for protecting the consumers. In this work, a screening method covering 116 human and veterinary drugs has been developed and validated in five types of animal feed at 0.02 and 0.2 mg kg-1.After a simple extraction with acetonitrile (1% formic acid) and subsequent ten-fold dilution with water, the sample extracts were analyzed by ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC-QTOF MS). Nearly all compounds tested were detected at 0.02 mg kg-1, based on the presence of the accurate-mass protonated molecule. The reliable identification of the compound using a second accurate-mass (fragment) ion was however more problematic at this level due to the lower abundance of the second ion in the mass spectra. In a subsequent step, the procedure was applied to 22 feed samples, where compounds as trimethoprim, robenidine, or -and β-nandrolone were detected and identified. The potential applicability of the method to quantitative analysis of the compounds detected in the samples was also evaluated.
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