Analysis of force curves obtained on the live cell membrane using chemically modified AFM probes

Afrin, Rehana; Yamada, Takafumi; Ikai, Atsushi

doi:10.1016/j.ultramic.2004.01.013

Cited by 63 publications

(42 citation statements)

References 32 publications

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“…This value is well below the force required to disrupt the membrane, which is on the order of 100 pN. 63 However, there are other mechanisms affecting the cells that rely on much weaker forces. 64 Forces of a few pN can cause changes …”

Section: Amf Treatmentmentioning

confidence: 95%

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Contreras¹,

Sougrat²,

Zaher³

et al. 2015

IJN

101

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In this paper, we show that magnetic nanowires with weak magnetic fields and low frequencies can induce cell death via a mechanism that does not involve heat production. We incubated colon cancer cells with two concentrations (2.4 and 12 μg/mL) of nickel nanowires that were 35 nm in diameter and exposed the cells and nanowires to an alternating magnetic field (0.5 mT and 1 Hz or 1 kHz) for 10 or 30 minutes. This low-power field exerted a force on the magnetic nanowires, causing a mechanical disturbance to the cells. Transmission electron microscopy images showed that the nanostructures were internalized into the cells within 1 hour of incubation. Cell viability studies showed that the magnetic field and the nanowires separately had minor deleterious effects on the cells; however, when combined, the magnetic field and nanowires caused the cell viability values to drop by up to 39%, depending on the strength of the magnetic field and the concentration of the nanowires. Cell membrane leakage experiments indicated membrane leakage of 20%, suggesting that cell death mechanisms induced by the nanowires and magnetic field involve some cell membrane rupture. Results suggest that magnetic nanowires can kill cancer cells. The proposed process requires simple and low-cost equipment with exposure to only very weak magnetic fields for short time periods.

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Section: Amf Treatmentmentioning

confidence: 95%

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Contreras¹,

Sougrat²,

Zaher³

et al. 2015

IJN

101

View full text Add to dashboard Cite

show abstract

“…A challenging experiment was that of Afrin et al [1157], who acquired force-displacement curves on the live cell surface with a tip functionalized with a covalent cross-linker, and have shown that only curves with the functionalized tip, and not the curves acquired with a normal, not functionalized tip, gave clear indication of prolonged adhesion which was terminated by a single step release.…”

Section: Rupture Force Of Specific Interactionsmentioning

confidence: 99%

Force measurements with the atomic force microscope: Technique, interpretation and applications

Butt

Cappella

Kappl

2005

Surface Science Reports

3,187

2,949

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“…For this purpose, we have to make sure that the weakest bond in the force spectroscopy system between the tip and the substrate is the targeted bond or interaction (Afrin et al , 2004. We therefore used the modified AFM tip with the amino-reactive bifunctional covalent cross-linkers, such as SPDP, sulfo-SPDP and DSS.…”

Section: Nanobiomechanics Of Proteins and Biomembrane A Ikai 2165mentioning

confidence: 99%

Nanobiomechanics of proteins and biomembrane

Ikai

2008

Phil. Trans. R. Soc. B

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A review of the work done in the Laboratory of Biodynamics of Tokyo Institute of Technology in the last decade has been summarized in this article in relation to the results reported from other laboratories. The emphasis here is the application of nanomechanics based on the force mode of atomic force microscopy (AFM) to proteins and protein-based biological structures. Globular proteins were stretched in various ways to detect the localized rigidity inside of the molecule. When studied by this method, bovine carbonic anhydrase II (BCA II), calmodulin and OspA protein all showed the presence of localized rigid structures inside the molecules. Protein compression experiments were done on BCA II to obtain an estimate of the Young modulus and its change in the process of denaturation. Then, the AFM probe method was turned on to cell membranes and cytoplasmic components. Force curves accompanying the extraction process of membrane proteins from intact cells were analysed in relation to their interaction with the cytoskeletal components. By pushing the AFM probe further into the cytoplasm, mRNAs were recovered from a live cell with minimal damage, and multiplied using PCR technology for their identification. Altogether, the work introduced here forms the basis of nanomechanics of protein and protein-based biostructures and application of the nanomechanical technology to cell biology.

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Analysis of force curves obtained on the live cell membrane using chemically modified AFM probes

Cited by 63 publications

References 32 publications

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Force measurements with the atomic force microscope: Technique, interpretation and applications

Nanobiomechanics of proteins and biomembrane

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