Analysis of flavonoids from propolis by on-line HPLC–electrospray mass spectrometry

Volpi, Nicola; Bergonzini, Gianluca

doi:10.1016/j.jpba.2006.04.017

Cited by 144 publications

(104 citation statements)

References 26 publications

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“…Furthermore, a single sample could take an hour to be analyzed. As most of the active components in propolis are polar, HPLC-ESI-MS is substituting GC-MS to compare A. mellifera propolis from different regions [9,10] as well as its plant origins [12]. Although this method requires less sample preparation than GC, a typical chromatographic analysis takes between 30 min and 1 h, using around 1 mL min −1 of solvent throughout.…”

Section: Resultsmentioning

confidence: 99%

“…For the majority of Asian or African countries, however, the composition and plant origins of propolis samples are only recently being studied [9][10][11]. Both gas chromatography coupled to mass spectrometry (GC/MS) [7] and high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC/ESI-MS) have been used to compare A. mellifera propolis from different regions [9,10] as well as its plant origins [12].…”

Section: Introductionmentioning

confidence: 99%

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Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

Sawaya

Abdelnur²,

Eberlin³

et al. 2010

Talanta

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a b s t r a c tChemical profiles of a representative set of 49 propolis ethanolic extracts collected worldwide (North and South America, Europe, Asia and Oceania) were obtained via easy ambient sonic-spray ionization mass spectrometry (EASI-MS). This simple and easily implemented fingerprinting technique analyses directly (without any pre-separation or sample manipulation) a tiny droplet of the ethanolic extract placed on a inert surface under ambient conditions. Data acquisition took about a minute per sample with no substantial sample carry-over. Extraction of propolis with ethanol by using an ultrasonic bath method gave EASI-MS data similar to the traditional maceration method, reducing total analysis time for the crude propolis resin from a week to just ca 1 h. Principal component analysis of the EASI-MS data is shown to group samples according to the plant sources of their resins, which characterizes their geographical origin.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

Sawaya

Abdelnur²,

Eberlin³

et al. 2010

Talanta

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“…A large number of analytical methods have been used for the analysis of phenolic compounds in propolis, including spectrophotometry [17], thin-layer chromatography [18], gas chromatography-mass spectrometry (GC-MS) [19], highperformance liquid chromatography (HPLC) [20], liquid chromatography-mass spectrometry (LC-MS) [21][22][23], electrospray ionization-mass spectrometry (ESI-MS) and electrospray ionization-tandem mass spectrometry (ESI-MS n ) [24], capillary electrophoresis [25], and nuclear magnetic resonance (NMR) [26]. Among these methods, MS with ESI is one of the most-used techniques in the research of natural products, as it is able to analyze complex mixtures because of its high selectivity.…”

Section: Introductionmentioning

confidence: 99%

“…The chemical composition of European propolis was determined by GC-MS [11,19], LC-MS, and ESI-MS [21][22][23][24]. Also Moreira et al [27] have recently described the phenolic content and the antioxidant potential of Portuguese propolis from two different regions, including the Northeast, but still, their phenolic components were not elucidated.…”

Section: Introductionmentioning

confidence: 99%

Phenolic characterization of Northeast Portuguese propolis: usual and unusual compounds

et al. 2009

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In this study, an ethanolic extract from Portuguese propolis was prepared, fractionated by highperformance liquid chromatography, and the identification of the phenolic compounds was done by electrospray mass spectrometry in the negative mode. This technical approach allowed the identification of 37 phenolic compounds, which included not only the typical phenolic acids and flavonoids found in propolis from temperate zones but also several compounds in which its occurrence have never been referred to in the literature. Four of the novel phenolic compounds were methylated and/or esterified or hydroxylated derivatives of common poplar flavonoids, although six peculiar derivatives of pinocembrin/pinobanksin, containing a phenylpropanoic acid derivative moiety in their structure, were also identified. Furthermore, the Portuguese propolis sample was shown to contain a p-coumaric ester derivative dimer.

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“…Many studies have analysed the propolis compounds using GC-MS (Murat 2002;Maciejewicz et al 2001;Park et al 2002) and HPLC methods (Osmany et al 2007;Medana et al 2008;Volpi & Bergonzini 2006) since the propolis compounds has varied polarity.…”

Section: Resultsmentioning

confidence: 99%

Chemical Constituents of Malaysian Apis mellifera Propolis

Ismail¹,

Sulaiman²,

Kannan³

et al. 2018

JSM

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Analysis of flavonoids from propolis by on-line HPLC–electrospray mass spectrometry

Cited by 144 publications

References 26 publications

Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

Phenolic characterization of Northeast Portuguese propolis: usual and unusual compounds

Chemical Constituents of Malaysian Apis mellifera Propolis

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