Analysis of flavonoids and phenolic acids in Greek aromatic plants: Investigation of their antioxidant capacity and antimicrobial activity

Proestos, Charalampos; Boziaris, Ioannis S.; Nychas, George‐John E.; Komaitis, Michael

doi:10.1016/j.foodchem.2005.01.049

Cited by 427 publications

(269 citation statements)

References 31 publications

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“…Phenolic acids are found in nature as esters and rarely as glycosides or in free form [11]. General comments have been published [12,13,14]. Thus hydrolysis was needed for the identification and quantitative determination of phenolic acids.…”

Section: Resultsmentioning

confidence: 99%

Aromatic Plants: Antioxidant Capacity and Polyphenol Characterisation

Proestos¹,

Varzakas²

2017

Preprint

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Abstract:The antioxidant properties and polyphenol content of some selected aromatic plants grown in Greece were studied. Plants were refluxed with 60% methanol after acid hydrolysis. The phenolic substances were quantified by HPLC-DAD. The antioxidant capacity of the extracts was determined with the Rancimat test using sunflower oil as substrate. Free radical scavenging activity was measured using the stable free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Results were compared with standard BHT and ascorbic acid. Total phenol concentration of the extracts was estimated with Folin-Ciocalteu reagent using gallic acid as standard.

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Section: Resultsmentioning

confidence: 99%

Aromatic Plants: Antioxidant Capacity and Polyphenol Characterisation

Proestos¹,

Varzakas²

2017

Preprint

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“…Ben Jemia et al (2013), Radulovi c et al (2012) and Proestos et al (2013) studied plants from the Geranium genus and obtained much lower TPC values than ours, 32.24 mg GAE/g dw for G. robertianum methanolic extract, 109.5 mg GAE/g dw for G. macrohrryzym ethanolic extract and 4.0 mg GAE/g dw for G. purpureum methanolic extract respectively. While our plant TPC was higher, its scavenging activity of the DPPH radical was lower, with an average of 205 lg/mL versus 19.98 lg/mL for G. robertianum methanolic extract and approximately 70 lg/mL for G. purpureum aqueous extract (Sohretoglu et al 2011). Proestos et al (2006 also studied the individual phenolics of G. purpureum, and found gallic acid to be present at a much lower concentration (14 mg/100 g).…”

Section: Antioxidant Activity Phenolic Profile and Trace Elementsmentioning

confidence: 87%

Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs

Nunes

Paśko

Tyszka-Czochara

et al. 2016

Pharmaceutical Biology

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Context: Crataegus monogyna L. (Rosaceae) (CM), Equisetum telmateia L. (Equisataceae) (ET), Geranium purpureum Vil. (Geraniaceae) (GP), Mentha suaveolens Ehrh. (Lamiaceae) (MS), and Lavandula stoechas L. spp. luisieri (Lamiaceae) (LS) are all medicinal. Objective: To evaluate the antioxidant, antiproliferative and antimicrobial activities of plant extracts and quantify individual phenolics and zinc. Material and methods: Aerial part extracts were prepared with water (W), ethanol (E) and an 80% mixture (80EW). Antioxidant activity was measured with TAA, FRAP and RP methods. Phenolics were quantified with a HPLC. Zinc was quantified using voltammetry. Antibacterial activity (after 48 h) was tested using Enterococcus faecalis, Bacillus cereus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Listeria monocytogenes. Antiproliferative activity (after 24 h) was tested using HEP G2 cells and fibroblasts. Results: Solvents influenced results; the best were E and 80EW. GP had the highest antioxidant activity (TAA and FRAP of 536.90 mg AAE/g dw and 783.48 mg TE/g dw, respectively). CM had the highest zinc concentration (37.21 mg/kg) and phenolic variety, with neochlorogenic acid as the most abundant (92.91 mg/100 g dw). LS was rich in rosmarinic acid (301.71 mg/100 g dw). GP and LS inhibited the most microorganisms: B. cereus, E. coli and S. aureus. GP also inhibited E. faecalis. CM had the lowest MIC: 5830 lg/mL. The antibacterial activity is explained by the phenolics present. LS and CM showed the most significant anti-proliferative activity, which is explained by their zinc content. Conclusion: The most promising plants for further studies are CM, LS and GP.ARTICLE HISTORY

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“…The oxidative stability of the oil was determined on a 743 Rancimat analyzer (Metrohm, Switzerland) according to a reported method (Proestos et al 2006). The oil (5 g) was subjected to oxidation at 110°C (air flow 20 L/h).…”

Section: Determination Of Oil Oxidative Stabilitymentioning

confidence: 99%

Preparation and characterization of foxtail millet bran oil using subcritical propane and supercritical carbon dioxide extraction

Shi

Zhang

et al. 2014

J Food Sci Technol

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The foxtail millet (Setaria italica Beauv) bran oil was extracted with traditional solvent extraction (SE), supercritical carbon dioxide extraction (SCE) and subcritical propane extraction (SPE) and analyzed the yield, physicochemical property, fatty acid profile, tocopherol composition, oil oxidative stability in this study. The yields of foxtail millet bran oil by SE, SCE and SPE were 17.14 %, 19.65 %, 21.79 % of raw material weight (corresponded to 75.54 %, 86.60 %, 96.03 % of the total amount of the oil measured by using Soxhlet extraction), respectively. The effect of the extraction methods on the physicochemical properties (peroxide value, saponification value and color) was significant while the difference in fatty acid profile was negligible based on GC analysis. The major components of vitamin E in the obtained oils were identified as α-and β-tocopherols by HPLC, and SPE was superior to SE and SCE in the extraction of tocopherols. In Rancimat test, the oil obtained by SPE showed the highest oil oxidative stability, which could attribute to its high tocopherol content and low peroxide value. In view of oil quality, SPE employed smaller times and lower pressures compared to SE and SCE. SPE was a suitable and selective method for the extraction of the foxtail millet bran oil.

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Analysis of flavonoids and phenolic acids in Greek aromatic plants: Investigation of their antioxidant capacity and antimicrobial activity

Cited by 427 publications

References 31 publications

Aromatic Plants: Antioxidant Capacity and Polyphenol Characterisation

Aromatic Plants: Antioxidant Capacity and Polyphenol Characterisation

Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs

Preparation and characterization of foxtail millet bran oil using subcritical propane and supercritical carbon dioxide extraction

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