Analysis of five rice 4-coumarate:coenzyme A ligase enzyme activity and stress response for potential roles in lignin and flavonoid biosynthesis in rice

Sun, Hongying; Liu, Ying; Feng, Shengqiu; Zou, Weihua; Guo, Kai; Fan, Chunfen; Si, Shengli; Peng, Liangcai

doi:10.1016/j.bbrc.2012.12.019

Cited by 102 publications

(80 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous studies, 4CLs showed similar divergent substrate specificity (Lee and Douglas 1996;Allina et al 1998;Hu et al 1998;Ehlting et al 1999;Cukovica et al 2001;Harding et al 2002;Lindermayr et al 2002;Hamberger and Hahlbrock 2004;Costa et al 2005;Gui et al 2011;Sun et al 2013). The consistency of these results suggests the universal existence of divergent substrate specificity of 4CLs isoforms.…”

Section: Discussionsupporting

confidence: 59%

“…4CL (4-coumarate/coenzyme A ligase; EC 6.2.1.12) catalyzes the esterification of 4-coumarate, caffeate, and ferulate. Plant 4CLs have been characterized from a range of species, with five from rice (Gui et al 2011;Sun et al 2013), three from soybean (Glycine max) (Lindermayr et al 2002), four from Arabidopsis thaliana (Ehlting et al 1999;Hamberger and Hahlbrock 2004;Costa et al 2005), two from tobacco (Lee and Douglas 1996), two from aspen (Populus tremuloides) (Hu et al 1998;Harding et al 2002), four from hybrid poplar (Populus trichocarpa×Populus deltoides) (Allina et al 1998;Cukovica et al 2001), and two from Ruta graveolens (Endler et al 2008). Although the 4CL genes of many plants have been characterized, the true size of the 4CL gene family in most plant species and the divergent roles of each member are not well understood.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Divergent and Overlapping Function of Five 4-Coumarate/Coenzyme A Ligases from Populus tomentosa

Rao

Pan

et al. 2014

Plant Mol Biol Rep

View full text Add to dashboard Cite

4-Coumarate/coenzyme A ligases (4CLs) play key roles in the biosynthesis of plant secondary compounds and act at the divergence point from general phenylpropanoid metabolism to several major branch pathways, such as lignins and flavonoids. In this study, five 4CL genes in Populus tomentosa were cloned and characterized. Quantitative polymerase chain reaction (qPCR) and promoter-β-glucuronidase analysis showed that the expression of Pto4CLs is significantly divergent and certainly overlapping. The five Pto4CL recombinant proteins also have different substrate specificities and catalytic turnover rates. Pto4CL4 has the highest substrate affinity and catalytic turnover rate compared with other Pto4CL proteins. All five Pto4CL recombinant proteins had no detectable activity towards sinapate. Deletion of V338 residues in Pto4CL mutants resulted in activity towards sinapate. The low accumulation of sinapate and high proportion of S unit lignin implies that the ability of 4CL to catalyze the activation of sinapate is not necessary for lignin biosynthesis. Overexpression of the five Pto4CLs in transgenic tobacco using the 35S promoter revealed significantly increased lignin contents and decreased hydroxycinnamate derivative contents in phloem, xylem, root, and leaf tissues. An increased content of naringenin in Pto4CL4 transgenic tobacco roots was also observed. Our results suggest that the substrate specificity and divergent and overlapping expression patterns of Pto4CL isoforms may play important roles in regulating the lignin and flavonoid biosynthesis.

show abstract

Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Divergent and Overlapping Function of Five 4-Coumarate/Coenzyme A Ligases from Populus tomentosa

Rao

Pan

et al. 2014

Plant Mol Biol Rep

View full text Add to dashboard Cite

show abstract

“…Substituted Ara may interact with the β-1,4-glucan chains in amorphous areas of cellulose micro fibrils via hydrogen bonding, especially on the non-KOH-extractable residues that consist of 10%-30% of total hemicelluloses. Therefore, cellulose crystallinity significantly affected by xly/ara ratio [57]. In addition, it has reported in rice mutants that the Ara level in non-KOHextractable residues by reducing the crystallinity of cellulose positively affects plant lodging resistance [16].…”

Section: Influence Of Plant Cell Wall Components On Biomass Digestibimentioning

confidence: 99%

Advances in Genetic Manipulation of Lignocellulose to Reduce Biomass Recalcitrance and Enhance Biofuel Production in Bioenergy Crops

Madadi¹,

Penga²,

Abbas³

2017

J Plant Biochem Physiol

View full text Add to dashboard Cite

Lignocellulose biomass derived from plant cell walls is a rich source of biopolymers for the production of biofuels. Biomass recalcitrance is the noticeable and main features of lignocellulose which can reduces by genetic modification of plant cell wall. The aim of the present review is to provide the reader a new insight for enhancing biomass yield and biofuels production. This can be issued by focusing on major perennial grasses, cereal crops and woody feedstock which have high biomass yield or large biomass residues and also the effects of distinctive cell wall polymers (cellulose, hemicellulose, lignin, and pectin) on the enzymatic saccharification of biomass under different pretreatments. Moreover the present review paper will also major gene candidates which are involved plant cell wall biosynthesis, degradation and modification for improving biomass yield and digestibility in transgenic plants and genetic mutants.

show abstract

“…Phenylpropanoid metabolism is regulated primarily by several corresponding genes. Similar to other genes, 4CL gene expression and its promoter activity are varied during tissue and cell differentiation, especially when the cells become specialized to produce phenylpropanoid-derived compounds (Pan et al 2013;Sun et al 2013). The regulation of the gene may be due to tissue-specific expression, different developmental stages or environmental stress (Chowdhury et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Besides involving in the developmental process, expression of different 4CL isoforms has been shown to affect the production of flavonoids and various monolignols in plants (Sun et al 2013;Li et al 2014). Therefore, elucidation of flavonoid biosynthesis at molecular level could lead to a better understanding of the process and to aid in enhancing the production of these valuable secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

Expression profiles of flavonoid-related gene, 4 coumarate: coenzyme A ligase, and optimization of culturing conditions for the selected flavonoid production in Boesenbergia rotunda

Ata

Yusuf

Tan

et al. 2015

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Knowing 4-Coumarate: coenzyme A ligase (4CL) is one of the important enzymes in the biosynthetic pathway of phenylpropanoid, we studied the gene expression profiles of 4CL and determined the production of selected flavonoids in different parts of Boesenbergia rotunda. The optimal growth conditions for B. rotunda cells were also investigated. In this study, we found that the 4CL transcript was expressed in leaves, rhizomes, and roots with highest expression level found in roots. In contrast, HPLC results showed that the highest total flavonoid contents (pinostrobin, pinocembrin and panduratin A) were recorded in rhizome followed by root and leaf samples. Since rhizome has been found to be a suitable source of explants for in vitro propagation, thus, we initiated sprout from rhizome in order to establish cell suspension cultures. Our study showed that cells grown in full strength Murashige and Skoog basal medium (pH 5.8) containing 1 mg/l 1-napththylacetic acid, 1 mg/l 6-benzyladenine, and 3 % sucrose produced maximal cell biomass and total flavonoid contents. We also found that the 4CL transcript levels for 14-and 10-day old phenylalanine-treated cultures were up-regulated for 22-and 17-fold, respectively, compared to 0-day, indicating the promotive effect of phenylalanine in enhancing the 4CL gene expression. This study provides necessary baseline data for 4CL gene expression profiles and optimal cell culture conditions for the flavonoid production.

show abstract

Analysis of five rice 4-coumarate:coenzyme A ligase enzyme activity and stress response for potential roles in lignin and flavonoid biosynthesis in rice

Cited by 102 publications

References 30 publications

Divergent and Overlapping Function of Five 4-Coumarate/Coenzyme A Ligases from Populus tomentosa

Divergent and Overlapping Function of Five 4-Coumarate/Coenzyme A Ligases from Populus tomentosa

Advances in Genetic Manipulation of Lignocellulose to Reduce Biomass Recalcitrance and Enhance Biofuel Production in Bioenergy Crops

Expression profiles of flavonoid-related gene, 4 coumarate: coenzyme A ligase, and optimization of culturing conditions for the selected flavonoid production in Boesenbergia rotunda

Contact Info

Product

Resources

About