Divergent and Overlapping Function of Five 4-Coumarate/Coenzyme A Ligases from Populus tomentosa

Rao, Guodong; Pan, Xiang; Xu, Fang; Zhang, Yongzhuo; Cao, Shan; Jiang, Xiangning; Lü, Hai

doi:10.1007/s11105-014-0803-4

Cited by 22 publications

(15 citation statements)

References 41 publications

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“…The 4CL gene family has been characterized in several plants, including Arabidopsis thaliana, Populus trichocarpa, Oryza sativa and Glycine max [11,[23][24][25]. Genes of this family have been reported to function not only in plant growth and development [16,22,26], but also in response to biotic and abiotic stresses [7,27]. However, no comprehensive analysis of the 4CL genes has been documented in G. hirsutum.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

Sun

Xiong

Zhang

et al. 2020

Preprint

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Background: The function of 4-coumarate-CoA ligases (4CL) under abiotic stresses has been studied in plants, however, limited is known about the 4CL genes in cotton (G. hirsutum L.) and their roles in response to drought stress.Results: We performed genome-wide identification of the 4CL genes in G. hirsutum and investigated the expression profiles of the identified genes in various cotton tissues and in response to stress conditions with an aim to identify 4CL gene(s) associated with drought tolerance. We identified 34 putative 4CL genes in G. hirsutum that were clustered into three classes. Genes of the same class usually share a similar gene structure and motif composition. Many cis-elements related to stress and phytohormone responses were found in the promoters of the Gh4CL genes. Of the 34 Gh4CL genes, 26 were induced by at least one abiotic stress and 10 (including Gh4CL7) were up-regulated under the polyethylene glycol (PEG) simulated drought stress conditions. Virus-induced gene silencing (VIGS) in cotton and overexpression (OE) in Arabidopsis thaliana were applied to investigate the biological function of Gh4CL7 in drought tolerance. The Gh4CL7-silencing cotton plants showed more sensitive to drought stress, probably due to decreased relative water content (RWC), chlorophyll content and antioxidative enzyme activity, increased stomatal aperture, and the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2). Arabidopsis lines overexpressing Gh4CL7, however, were more tolerant to drought treatment, which was associated with improved antioxidative enzyme activity, reduced accumulation of MDA and H2O2 and up-regulated stress-related genes under the drought stress conditions. In addition, compared to their respective controls, the Gh4CL7-silencing cotton plants and the Gh4CL7-overexpressing Arabidopsis lines had a ~20% reduction and a ~10% increase in lignin content, respectively. The expression levels of genes related to lignin biosynthesis, including PAL, CCoAOMT, COMT, CCR and CAD, were lower in Gh4CL7-silencing plants than in controls. Taken together, these results demonstrated that Gh4CL7 could positively respond to drought stress and therefore might be a candidate gene for improvement of drought tolerance in cotton.Conclusion: We characterized the 4CL gene family in upland cotton and revealed a role of Gh4CL7 in lignin biosynthesis and drought tolerance.

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Section: Discussionmentioning

confidence: 99%

Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

Sun

Xiong

Zhang

et al. 2020

Preprint

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“…The 4CL gene family has been characterized in several plants, including Arabidopsis thaliana, Populus trichocarpa, Oryza sativa and Glycine max [12,[29][30][31]. Genes of this family have been reported to function not only in plant growth and development [13,28,[32][33], but also in response to biotic and abiotic stresses [7,34,35]. However, no comprehensive analysis of the 4CL genes has been documented in G. hirsutum.…”

Section: Discussionmentioning

confidence: 99%

“…Gh4CL7 belongs to class I whose genes have been shown to regulate lignin synthesis [21,28]. We investigated whether or not Gh4CL7 is also involved in lignin biosynthesis by comparing the lignin contents of the Gh4CL7-OE Arabidopsis lines and TRV:Gh4CL7 cotton plants with that of their corresponding control plants.…”

Section: Gh4cl7 Plays An Important Role In Lignin Biosynthesismentioning

confidence: 99%

Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

Sun

Xiong

Zhang

et al. 2019

Preprint

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Background The function of 4-coumarate-CoA ligases (4CL) under abiotic stresses have been studied in plants, however, limite is known about the upland cotton 4CL genes and their roles in response to drought stress. ResultsIn this study, we performed genome-wide identification of the 4CL genes in G. hirsutum and investigated the expression profiles of the identified genes in various cotton tissues and in response to stress conditions with an aim to identify 4CL gene(s) associated with drought tolerance. We identified 34 putative 4CL genes in G. hirsutum that were clustered into three classes. Genes of the same class usually share a similar gene structure and motif composition. Many cis -elements related to stress and phytohormone responses were found in the promoters of the Gh4CL genes. Of the 34 Gh4CL genes, 26 were induced by at least one abiotic stress and 10 were up-regulated under the PEG simulated drought stress conditions, including Gh4CL7 . Virus-induced gene silencing (VIGS) in cotton and overexpression (OE) in Arabidopsis thaliana were applied to investigate the biological function of Gh4CL7 in drought tolerance. The Gh4CL7 -silencing cotton plants showed more sensitive to drought stress, probably due to decreased relative water content (RWC), chlorophyll content and antioxidative enzyme activity, increased stomatal aperture, and the contents of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ). Arabidopsis lines overexpressing Gh4CL7 , however, were more tolerant to drought treatment, which was associated with improved antioxidative enzyme activity, reduced accumulation of MDA and H 2 O 2 and up-regulated stress-related genes under the drought stress conditions. In addition, compared to their respective controls, the Gh4CL7 -silencing cotton plants and the Gh4CL7-overexpressing Arabidopsis lines had a lower and higher lignin content, respectively. Taken together, these results demonstrated that Gh4CL7 could positively respond to drought stress and therefore might be a candidate gene for improvement of drought tolerance in cotton.Conclusion We comprehensively identified 4CL family genes in upland cotton and provide valuable information on the evolutionary relationship. Gh4CL7 gene is involved in lignin biosynthesis and play a positively role in reponsing drought stress.

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“…Furthermore, Li et al (2015) showed that the At4CL1 and At4CL3 genes of Arabidopsis were both involved in the biosynthesis of sinapoylmalate. In Populus tomentosa, its five Pto4CL genes also displayed an overlapping function in lignin biosynthesis (Rao et al 2015). The existence of multiple 4CL genes could be viewed as a strategy to safe-guide the integrity of important metabolic pathways that serve for plant growth and development like lignification where a loss-of-function mutation in one copy of the gene could be rectified by another copy.…”

Section: Gtacmentioning

confidence: 99%

“…The type I 4CL genes are responsible for lignification while the type II 4CL genes are involved in the biosynthesis of flavonoid compounds. Suppression of the type I 4CL genes led to a significant reduction in lignin content in different plants (Gui et al 2011;Xu et al 2011a), while overexpression increased the lignin content (Rao et al 2015). On the other hand, suppression of the type II 4CL gene only led to a reduction in eugenol content in Ocimum sanctum, without affecting the lignin content (Rastogi et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Cloning and Analysis of the Eg4CL1 Gene and Its Promoter from Oil Palm (Elaeis guineensis Jacq.)

Yu¹,

Seman²,

Shukor³

et al. 2018

JSM

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The empty fruit bunches of oil palm have been used as the raw material to produce biofuel. However, the lignin present in oil palm tissues hampers the enzymatic saccharification of lignocellulosic biomass and lower the yield of biofuel produced. Hence, various efforts were taken to identify the lignin biosynthetic genes in oil palm and to investigate their regulation at the molecular level. In this study, a lignin biosynthetic gene, Eg4CL1 and its promoter were isolated from the oil palm. Eg4CL1 contains the acyl-activating enzyme consensus motif and boxes I & II which are present in other 4CL homologs. Eg4CL1 was clustered together with known type I 4CL proteins involved in lignin biosynthesis in other plants. Gene expression analysis showed that Eg4CL1 was expressed abundantly in different organs of oil palm throughout the course of development, reflecting its involvement in lignin biosynthesis in different organs at all stages of growth. The presence of the lignification toolbox-AC elements in the 1.5 kb promoter of Eg4CL1 further suggests the potential role of the gene in lignin biosynthesis in oil palm. Together, these results suggested that Eg4CL1 is a potential candidate gene involved in lignin biosynthesis in oil palm.

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Divergent and Overlapping Function of Five 4-Coumarate/Coenzyme A Ligases from Populus tomentosa

Cited by 22 publications

References 41 publications

Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

Cloning and Analysis of the Eg4CL1 Gene and Its Promoter from Oil Palm (Elaeis guineensis Jacq.)

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