Analysis of expressed sequence tags from Centella asiatica (L.) Urban hairy roots elicited by methyl jasmonate to discover genes related to cytochrome P450s and glucosyltransferases

Kim, Ok-Tae; Um, Yurry; Jin, Mei Lan; Kim, Young Chang; Bang, Kyong-Hwan; Hyun, Dong Yun; Lee, Hyoung Seok; Lee, Yi

doi:10.1007/s11816-013-0311-2

Cited by 19 publications

(9 citation statements)

References 33 publications

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“…Both saponin and triterpenoid formation are catalyzed by cytochrome P450 enzymes. Asiatic acid and madecassic acid are glycosylated by UDP‐glucosyltransferases (UGTs), which link the trisaccharide (Rha+Glc+Glc) at the C‐28 position to obtain asiaticoside and madecassoside .…”

Section: Biosynthesis Of Centellosidesmentioning

confidence: 99%

“…The most recent attempts to boost centelloside production have focused on metabolic engineering. An important drawback of hairy root cultures of C. asiatica is that centellosides form and accumulate mainly in the aerial part of the plant, but various research groups have tackled this problem by designing hairy root systems coupled with elicitation .…”

Section: Introductionmentioning

confidence: 99%

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Biotechnological production of centellosides in cell cultures of Centella asiatica (L) Urban

Gallego

Ramírez-Estrada

Vidal-Limon

et al. 2014

Engineering in Life Sciences

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Centella asiatica (L.) Urban plants have been used since ancient times for their medicinal properties, and their extracts have proven antioxidant, wound healing, sedative, and neuroprotective activities, among others. The natural compounds responsible for C. asiatica bioactivity are triterpene saponins formed from the dammarene branch of the triterpene biosynthetic pathway, collectively known as centellosides, with madecassoside and asiaticoside and their aglycones, madecassic acid and asiatic acid being the most important. Several biotechnological approaches have been developed for the bioproduction of centellosides, based on cell, hairy root, and in vitro plant cultures. This review summarizes the main therapeutic properties of these compounds, as well as their biosynthetic pathways, referring to genetic studies that have identified genes involved in their formation. The biotechnological production of centellosides from a small scale to bioreactor level is also covered. Finally, we summarize the most effective strategies for increasing centelloside yield, including recent transcriptomic, proteomic, and metabolomic studies that have gained new insights into the centelloside biosynthetic pathway and its control.

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Section: Biosynthesis Of Centellosidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biotechnological production of centellosides in cell cultures of Centella asiatica (L) Urban

Gallego

Ramírez-Estrada

Vidal-Limon

et al. 2014

Engineering in Life Sciences

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“…Investigation of transcriptomes provided evidence about the role of jasmonates in inducing secondary metabolism. Analysis of MeJA-induced transcriptomes in Polygonum minus 34 , Panax quinquefolius 35 , Centella asiatica 36 , and Salvia miltiorrhiza Bunge 37 demonstrated that genes involved in secondary metabolism were up-regulated. In addition, application of 0.5% (w/w) MeJA in lanolin paste on tomatoes in the mature green (MG) stage resulted in lycopene accumulation 38 .…”

Section: Introductionmentioning

confidence: 99%

De novo Transcriptome Sequencing of MeJA-Induced Taraxacum koksaghyz Rodin to Identify Genes Related to Rubber Formation

Cao¹,

Yan²,

Lei³

et al. 2017

Sci Rep

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Increase in the consumption of natural rubber (NR) has necessitated the identification of alternative sources of NR. The quality of NR produced by Taraxacum koksaghyz Rodin (TKS) is comparable to that from Hevea brasiliensis (H.brasiliensis), and therefore, TKS is being considered as an alternative source of NR. Here, we sequenced the TKS root transcriptome after wild TKS seedlings were treated with methyl jasmonate (MeJA) for 0, 6, and 24 h. The clean reads generated for each experimental line were assembled into 127,833 unigenes. The Kyoto encyclopedia of genes and genomes pathway prediction suggested that methyl jasmonate regulated secondary metabolism in TKS. Differential expression analysis showed that the expression of HMGCR, FPPS, IDI, GGPPS, and REF/SRPP increased with methyl jasmonate treatment. Interestingly, differential expression analysis of the jasmonate (JA)-related transcription factors (TFs), indicated that certain genes encoding these transcription factors (namely, bHLH, MYB, AP2/EREBP, and WRKY) showed the same expression pattern in the lines treated for 6 h and 24 h. Moreover, HMGCR was up-regulated in the transgenic seedlings overexpressing DREB. We predicted that methyl jasmonate regulated secondary metabolism and affected rubber biosynthesis via the interaction of the JA-related TFs with genes associated with rubber biosynthesis in TKS.

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“…The herb has been well documented mostly due to its medicinal properties but also as a nutritional and industrial crop ( Hashim, 2011 ; Gallego et al, 2014 ). Various therapeutic properties of the herb have been extensively documented ( James and Dubery, 2009 ; Gallego et al, 2014 ; Gupta et al, 2014 ), where recently, it has also been reported to possess neuroprotective properties in humans and animal models ( Gao et al, 2009 ; Omar et al, 2011 ; Kim et al, 2013 ; Gallego et al, 2014 ; Prasad et al, 2014 ). The various bio-active properties of C. asiatica are attributed to the composition of its secondary metabolite profile.…”

Section: Introductionmentioning

confidence: 99%

Stimulatory Effects of Acibenzolar-S-Methyl on Chlorogenic Acids Biosynthesis in Centella asiatica Cells

et al. 2016

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Centella asiatica is a perrenial herb that grows in tropical regions with numerous medicinal properties mostly attributed to the presence of pentacyclic triterpenoids. Interestingly, this plant also possess a significant amount of phenylpropanoid-derived chlorogenic acids (CGAs) that have recently been reported to confer neuroprotective properties. In a biotechnological attempt to increase the biosynthesis of CGA-derivatives in cultured Centella cells, acibenzolar-S-methyl was applied as a xenobiotic inducer in combination with quinic acid and shikimic acid as precursor molecules. Applying a semi-targeted metabolomics-based approach, time and concentration studies were undertaken to evaluate the effect of the manipulation on cellular metabolism leading to CGA production. Phytochemical extracts were prepared using methanol and analyzed using a UHPLC-qTOF-MS platform. Data was processed and analyzed using multivariate data models. A total of four CGA-derivatives, annotated as trans-5-feruloylquinic acid, 3,5 di-caffeoylquinic acid, 3,5-O-dicaffeoyl-4-O-malonylquinic acid (irbic acid) and 3-caffeoyl, 5-feruloylquinic acid, were found to be upregulated by the acibenzolar-S-methyl treatment. To the best of our knowledge, this is the first report on the induction of CGA derivatives in this species. Contrary to expectations, the effects of precursor molecules on the levels of the CGAs were insignificant. However, a total of 16 metabolites, including CGA derivatives, were up-regulated by precursor treatment. Therefore, this study shows potential to biotechnologically manipulate C. asiatica cells to increase the production of these health beneficial CGAs.

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Analysis of expressed sequence tags from Centella asiatica (L.) Urban hairy roots elicited by methyl jasmonate to discover genes related to cytochrome P450s and glucosyltransferases

Cited by 19 publications

References 33 publications

Biotechnological production of centellosides in cell cultures of Centella asiatica (L) Urban

Biotechnological production of centellosides in cell cultures of Centella asiatica (L) Urban

De novo Transcriptome Sequencing of MeJA-Induced Taraxacum koksaghyz Rodin to Identify Genes Related to Rubber Formation

Stimulatory Effects of Acibenzolar-S-Methyl on Chlorogenic Acids Biosynthesis in Centella asiatica Cells

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