Analysis of Diversity of Golden Potato Cyst Nematode (Globodera rostochiensis) Populations from Poland using Molecular Approaches

Abstract

Globodera rostochiensis is one of the most important plant parasitic nematodes, worldwide. As a quarantine pest of solanaceous crops, the species is often subjected to the morphological and genetic analysis as well as biological tests. They constitute the basis for this nematode detection and control. This paper presents the results of the study on variability of 16 populations of golden potato cyst nematode from Poland -one of the important potato producers -using molecular techniques. The cluster analysis based on sequencing of rDNA fragment showed slight differences among investigated populations from various localizations of the country. The ITS1 and ITS2 regions of rDNA were similar among populations of G. rostochiensis and differed in 1-3% of 1000-bp sequence. The analysis of populations polymorphism based on the RAPD technique also showed differences among populations of G. rostochiensis, indicating that climatic conditions of the mountainous area might have influence on genetic variability.

A Comparative and Phylogenetic Study of the Ditylenchus dipsaci, Ditylenchus destructor and Ditylenchus gigas Populations Occurring in Poland

“…For each sample, a few adult (or a few dozen larvae) nematodes were used for DNA isolation, as described previously (Nowaczyk et al. ). Purified DNA was used as a template in PCR.…”

Molecular Methods of Characterization and Identification of Globodera Rostochiensis and Globodera Pallida Populations

“…Low variation of the ITS-1 sequences and high conservativeness of this genome region failed to distinguish the isolates groups within the species with the used of this particular method. Comparative analysis of rDNA (fragments ITS1/5.8S/ ITS2) sequences of 16 polish Globodera populations show 97-98% identify in G. rostochiensis and 94-95% identity in G. pallida isolates and allow for splitting populations into three phylogenetic groups originate from Central and Eastern part of the country (Nowaczyk et al, 2011). Molecular characterization of Serbian Globodera isolates reported by Oro et al (2012) based on comparison of variation occurred in ITS-1 region assumed that possible ancestors of PCN population originated from Peru but presence of G. pallida is not the result of direct import of infected potatoes from Latin America but from England where the population from York has the same sequence like Serbian ones.…”

RNA-Seq of Cyst Nematode Infestation of Potato (Solanum tuberosum L.): A Comparative Transcriptome Analysis of Resistant and Susceptible Cultivars

“…Moreover, the feeding site (i.e., syncytium) formed by the PCN is metabolically active, acts as a "nutrient sink", and attracts nutrients produced by plants [10]. In other words, the plant spends major portions of its energy supporting the PCN at the cost of itself, which results in heavy yield losses of up to 80% [11,12].…”