A Comparative and Phylogenetic Study of the Ditylenchus dipsaci, Ditylenchus destructor and Ditylenchus gigas Populations Occurring in Poland

Abstract

The genus Ditylenchus contains more than 80 recognized nematode species with a very wide host range. The most serious species are Ditylenchus dipsaci and Ditylenchus destructor. Populations of D. dipsaci species complex were collected from Allium cepa, Cichorium endivia and Phlox paniculata in Poland. The Ditylenchus gigas population was collected from Vicia faba minor, and populations of D. destructor, from Solanum tuberosum spp. tuberosum. Analyses of the rDNA sequences spanning both ITS1 and ITS2 fragment regions were carried out on the collected populations. The obtained DNA sequences were compared with those DNA sequences deposited in GenBank of populations isolated in other countries. Phylogenetic analysis was performed using the data obtained from the DNA sequence comparisons. The results indicated that there is no clear distinction between European and non‐European populations within D. dipsaci. The results also showed no clear distinction between populations isolated from different host plant species, including populations found in Poland. The populations of D. destructor described here constitute a common group together with American and Chinese populations belonging to the haplotype C of the D. destructor species. On the other hand, the D. gigas population was localized separately from those populations that have been described up until now, from Europe and Africa. This is also the first report on the occurrence of D. gigas in Poland.

A fast and sensitive method for the simultaneous identification of three important nematode species of the genusDitylenchus

“…Of particular wileyonlinelibrary.com/journal/ps interest are those pests that should be eliminated or contained owing to their quarantine status, which imposes an absolute legal obligation in the European Union to limit and eradicate plants infested with such pests. rDNA sequences contain readily detectable genetic markers in the form of the tandem repeats that are used for construction of phylogenetic trees, 4 for the assessment of genetically related populations and for diagnostic purposes. 17 These markers have been used in the present assay so that all D. dipsaci and D. gigas populations and all D. destructor haplotypes described in GenBank can be detected.…”

“…minor). 4 Its occurrence on other legume plants is therefore possible. This nematode, formerly considered to be a 'giant race' of D. dipsaci (Ditylenchus sp.…”

Ditylenchus laurae sp. n. (Tylenchida: Anguinidae) from Poland – a new species of the D. dipsaci complex associated with a water plant, Potamogeton perfoliatus L.

“…PCR products were separated on 1% agarose gel and PCR products were excised from the gel followed by elution using QIAquick ® Gel Extraction Kit (Qiagen). Subsequently, products were cloned and transformed to E. coli cells as described previously (Jeszke et al, 2014). Recombinant plasmids were purified from bacterial colonies using QiaPrep Spin Miniprep Kit (Qiagen) and sequenced.…”

PM 7/87 (2) Ditylenchus destructor and Ditylenchus dipsaci

“…On the basis of morphological, biochemical, molecular and karyological analyses a total of 13 nominal species have been synonymized and up to 30 host races of D. dipsaci s.l. have been suggested, divided into two groups (Jeszke et al, 2013). The larger group, named D. dipsaci sensu stricto (s.s.), comprises diploidal populations characterized by their 'normal' size.…”

“…The second group is polyploidal and currently comprises Ditylenchus gigaspreviously known 1 Use of names of chemicals or equipment in these EPPO Standards implies no approval of them to the exclusion of others that may also be suitable. as the 'giant race' of D. dipsaci parasitizing Vicia faba (broad bean) (Vovlas et al, 2011), D. weischeri parasitizing Cirsium arvense (creeping thistle) (Chizhov et al, 2010) and three undescribed Ditylenchus spp., which are associated with plant species of the Fabaceae, Asteraceae and Plantaginaceae, respectively (Jeszke et al, 2013). This protocol includes information to distinguish between the most important plant pests within this complex -D. dipsaci s.s. and D. gigas.…”