Analysis of Dispersion Mechanism in Gel Chromatography

Dispersion Mechanism in Gel Chromatography of Proteins

“…A channeling in a column. might play an important role as observed in the previous study on dispersion of low-molecular substances, and as a presumable consequence D. became lower than D 4) g'…”

“…As described in a previous paper, 4) DzJu values for NaCI were scattered around the theoretical line, i.e., DzJu d p • The elution curves for NaCl were quite symmetrical and the prolonged tail was negligibly small. The values for Blue Dextran were slightly higher than the theoretical line owing to the weak adsorption on Sephadex gel and the distribution of molecular weights.…”

“…When an injection time of sample is negligibly short and the effect of mass transfer across the mobile-gel phase interface is ignored, 1-'1' and 112 are given by Eqs. (3) and (4). 4.7) where…”

Effect of particle size distribution on column efficiency in gel chromatography.

“…In a column packed with gels having diameters represented by distribution function f(Dp), i.e., 5 f(Dp)dDp-1, the mass 0 balance equations of a solute in the mobile phase and in the gel phase are expressed by Eqs. (1) and 2, respectively.…”

Separação da mistura binaria proteica da alfa-lactalbumina e beta-lactoglobulina por cromatografia de exclusão molecular utilizando reciclo externo estacionario

“…Os valores são apresentados nas Figuras 5.13 a 5.17 e na tabela 5.1 O. Os pontos experimentais geraram comportamento linear com valores aproximados de coeficiente angular e linear, o que implica na aplicabilidade da Equação (3.33). No entanto segundo Nakanishi, et al (1977) considerando que o coeficiente de transferência de massa (kr) é razoavelmente grande, o termo 5/KfR é muito pequeno, o que faz com que a Equação tome a forma: Figura 5.9 Resultados experimentais obtidos para o segundo momento para a alactalbumina com concentração de 1,0 mg/mL em um leito de resina com h= 6,4 em apresentando o ajuste linear proposto pela Equação (5.4).…”