Effect of particle size distribution on column efficiency in gel chromatography.

Nakanishi, Koji; Yamamoto, Shuichi; Matsuno, Ryuichi; Kamikubo, Tudashi

doi:10.1271/bbb1961.42.1943

Cited by 10 publications

References 2 publications

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Pulse response in an immobilized‐enzyme column: Elution profiles in reversible and consecutive reactions

Adachi

Hashimoto

Miyai

et al. 1981

Biotech & Bioengineering

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SummaryA pulse of substrate solution was applied to an immobilized-enzyme column, in which the substrate was then converted by reversible or consecutive reactions. Immobilized glucose isomerase was used for the reversible reaction, and immobilized invertase and glucose oxidase for the consecutive reactions. The elution profiles of substrate and product were determined experimentally. These profiles were in good agreement with the ones predicted theoretically. The effect of some parameters on the elution profiles for reversible and consecutive reactions is discussed.

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Pulse response in an immobilized‐enzyme column: Elution profiles in reversible and consecutive reactions

Adachi

Hashimoto

Miyai

et al. 1981

Biotech & Bioengineering

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Ion exchange chromatography of proteins—predictions of elution curves and operating conditions. II. Experimental verification

Yamamoto

Nakanishi

Matsuno

et al. 1983

Biotech & Bioengineering

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The applicability and validity of the model developed in Part I were confirmed experimentally. In this article, various proteins were eluted both by stepwise and linear gradient elution on DEAE ion exchangers under a variety of experimental conditions. Adsorption isotherms were measured as a function of ionic strength in batch experiments. The moment method was employed for the determination of various parameters such as the gel-phase diffusion coefficient and the longitudinal dispersion coefficient. By use of these parameters and the experimentally measured ionic strength of the peak position, the number of plates was determined according to the method described in Part I. Theoretical elution curves were calculated with the experimentally measured adsorption equilibria and the number of plates. Good agreement was observed between theory an experiments. Various factors affecting the separation were investigated. It was found that the effect of the number of plates for salts, N'(p), was negligible except the case of stepwise elution of high ionic strength buffer. When elution curves were symmetrical, the widths of the elution curves were inversely proportional to the square root of the number of plates of proteins, N(p), as in other chromatographic techniques. A simple graphical method for prediction of the peak position in linear gradient elution described in Part I was found applicable when the elution curves were symmetrical. A useful correlation of prediction of the peak width in a linear gradient elution was proposed on the basis of the approximate solution derived in Part I of this study. This graphical method and correlation permit easy prediction of the peak position and peak width in linear gradient elution in the case of symmetrical elution curves.

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Predicting the Performance of Production-Scale Chromatography

Yamamoto

Sano

1992

Biochemical Engineering for 2001

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Effect of particle size distribution on column efficiency in gel chromatography.

Abstract: Column efficiency in gel chromatography is influenced by a number of factors such as particle size, flow rate and degree of cross-linkage of gels. Particle size distribution of packing materials is also considered one of the important factors. It has been empirically found

Cited by 10 publications

References 2 publications

Pulse response in an immobilized‐enzyme column: Elution profiles in reversible and consecutive reactions

Pulse response in an immobilized‐enzyme column: Elution profiles in reversible and consecutive reactions

Ion exchange chromatography of proteins—predictions of elution curves and operating conditions. II. Experimental verification

Predicting the Performance of Production-Scale Chromatography

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