Analysis of digital gene expression profiling in the gonad of male silkworms (Bombyx mori) under fluoride stress

Tang, Wenchao; Xiao, Yuanyuan; Li, Guannan; Zheng, Xiaokun; Yin, Yulong; Wang, Lingyan; Zhu, Yong

doi:10.1016/j.ecoenv.2018.01.028

Cited by 18 publications

(6 citation statements)

References 68 publications

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“…ABC transporters are membrane proteins that are divided into eight subfamilies (A to H) based on the conserved nucleotide-binding domain (NBD) and their functions [46]. Three ABCG subfamily genes are upregulated in H. armigera after being treated with abamectin, indoxacarb, and lambda-cyhalothrin [47].…”

Section: Discussionmentioning

confidence: 99%

Growth inhibition of Spodoptera frugiperda larvae by camptothecin correlates with alteration of the structures and gene expression profiles of the midgut

Shu

Zou

et al. 2021

BMC Genomics

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Background Spodoptera frugiperda is a serious pest that causes devastating losses to many major crops, including corn, rice, sugarcane, and peanut. Camptothecin (CPT) is a bioactive secondary metabolite of the woody plant Camptotheca acuminata, which has shown high toxicity to various pests. However, the effect of CPT against S. frugiperda remains unknown. Results In this study, bioassays have been conducted on the growth inhibition of CPT on S. frugiperda larvae. Histological and cytological changes were examined in the midgut of larvae fed on an artificial diet supplemented with 1.0 and 5.0 µg/g CPT. The potential molecular mechanism was explored by comparative transcriptomic analyses among midgut samples obtained from larvae under different treatments. A total of 915 and 3560 differentially expressed genes (DEGs) were identified from samples treated with 1.0 and 5.0 µg/g CPT, respectively. Among the identified genes were those encoding detoxification-related proteins and components of peritrophic membrane such as mucins and cuticle proteins. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses indicated that part of DEGs were involved in DNA replication, digestion, immunity, endocrine system, and metabolism. Conclusions Our results provide useful information on the molecular basis for the impact of CPT on S. frugiperda and for future studies on potential practical application.

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Section: Discussionmentioning

confidence: 99%

Growth inhibition of Spodoptera frugiperda larvae by camptothecin correlates with alteration of the structures and gene expression profiles of the midgut

Shu

Zou

et al. 2021

BMC Genomics

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“…Genes were identified as differentially expressed (i.e. DEGs) if the adjusted P -value was <0.05 40 . The functional annotation and classification of the genes were performed using the GO database, and biological pathway annotations were obtained using the KEGG database.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome-based identification and characterization of genes responding to imidacloprid in Myzus persicae

Meng

Chen

Zhang

2019

Sci Rep

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Myzus persicae is a serious and widespread agricultural pest, against which, imidacloprid remains an effective control measure. However, recent reports indicate that this aphid has evolved and developed resistance to imidacloprid. This study aimed to elucidate the underlying mechanisms and genetic basis of this resistance by conducting comparative transcriptomics studies on both imidacloprid-resistant (IR) and imidacloprid-susceptible (IS) M. persicae. The comparative analysis identified 252 differentially expressed genes (DEGs) among the IR and IS M. persicae transcriptomes. These candidate genes included 160 and 92 genes that were down- and up-regulated, respectively, in the imidacloprid-resistant strain. Using functional classification in the GO and KEGG databases, 187 DEGs were assigned to 303 functional subcategories and 100 DEGs were classified into 45 pathway groups. Moreover, several genes were associated with known insecticide targets, cuticle, metabolic processes, and oxidative phosphorylation. Quantitative real-time PCR of 10 DEGs confirmed the trends observed in the RNA sequencing expression profiles. These findings provide a valuable basis for further investigation into the complicated mechanisms of imidacloprid resistance in M. persicae.

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“…Up-regulation of ribosomal proteins in response to sustained stress has been found in the Pacific oyster, suggesting an effort to increase translation capacity or protect ribosomal function through the addition or replacement of ribosomal proteins [70]. Up-regulation of ribosomal related genes was previously found in Daphnia as a response to stress induced by predation [82] and in silkworms as a response to stress induced by fluoride [83]. This suggests that ribosomal gene products are important for metabolic adjustments during stress in general.…”

Section: Discussionmentioning

confidence: 99%

Within species expressed genetic variability and gene expression response to different temperatures in the rotifer Brachionus calyciflorus sensu stricto

et al. 2019

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Genetic divergence is impacted by many factors, including phylogenetic history, gene flow, genetic drift, and divergent selection. Rotifers are an important component of aquatic ecosystems, and genetic variation is essential to their ongoing adaptive diversification and local adaptation. In addition to coding sequence divergence, variation in gene expression may relate to variable heat tolerance, and can impose ecological barriers within species. Temperature plays a significant role in aquatic ecosystems by affecting species abundance, spatio-temporal distribution, and habitat colonization. Recently described (formerly cryptic) species of the Brachionus calyciflorus complex exhibit different temperature tolerance both in natural and in laboratory studies, and show that B. calyciflorus sensu stricto (s.s.) is a thermotolerant species. Even within B. calyciflorus s.s., there is a tendency for further temperature specializations. Comparison of expressed genes allows us to assess the impact of stressors on both expression and sequence divergence among disparate populations within a single species. Here, we have used RNA-seq to explore expressed genetic diversity in B. calyciflorus s.s. in two mitochondrial DNA lineages with different phylogenetic histories and differences in thermotolerance. We identify a suite of candidate genes that may underlie local adaptation, with a particular focus on the response to sustained high or low temperatures. We do not find adaptive divergence in established candidate genes for thermal adaptation. Rather, we detect divergent selection among our two lineages in genes related to metabolism (lipid metabolism, metabolism of xenobiotics).

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Analysis of digital gene expression profiling in the gonad of male silkworms (Bombyx mori) under fluoride stress

Cited by 18 publications

References 68 publications

Growth inhibition of Spodoptera frugiperda larvae by camptothecin correlates with alteration of the structures and gene expression profiles of the midgut

Growth inhibition of Spodoptera frugiperda larvae by camptothecin correlates with alteration of the structures and gene expression profiles of the midgut

Transcriptome-based identification and characterization of genes responding to imidacloprid in Myzus persicae

Within species expressed genetic variability and gene expression response to different temperatures in the rotifer Brachionus calyciflorus sensu stricto

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