Analysis of differentially expressed genes in Curcuma amada and Zingiber officinale upon infection with Ralstonia solanacearum by suppression subtractive hybridization

Prasath, D.; Suraby, Erinjery Jose; Karthika, R.; Rosana, O. B.; Prameela, T. P.; Anandaraj, M.

doi:10.1007/s11738-013-1362-2

Cited by 13 publications

(4 citation statements)

References 35 publications

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“…Previous studies [47]–[49] showed that genes involved in lignin synthesis were over-expressed in various plants when they challenged with pathogens. These results suggest that the interaction between C. amada and R. solanacearum induces lignin accumulation, probably as a physical barrier.…”

Section: Discussionmentioning

confidence: 99%

“…The category of genes that seems to be involved in C. amada resistance to the bacterium encodes proteins involved in lignin biosynthesis such as cytochrome P450, succinyl-CoA ligase and S-adenosylmethionine synthase 1. Previous studies [47] – [49] showed that genes involved in lignin synthesis were over-expressed in various plants when they challenged with pathogens. These results suggest that the interaction between C. amada and R. solanacearum induces lignin accumulation, probably as a physical barrier.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of the Transcriptomes of Ginger (Zingiber officinale Rosc.) and Mango Ginger (Curcuma amada Roxb.) in Response to the Bacterial Wilt Infection

et al. 2014

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Bacterial wilt in ginger (Zingiber officinale Rosc.) caused by Ralstonia solanacearum is one of the most important production constraints in tropical, sub-tropical and warm temperature regions of the world. Lack of resistant genotype adds constraints to the crop management. However, mango ginger (Curcuma amada Roxb.), which is resistant to R. solanacearum, is a potential donor, if the exact mechanism of resistance is understood. To identify genes involved in resistance to R. solanacearum, we have sequenced the transcriptome from wilt-sensitive ginger and wilt-resistant mango ginger using Illumina sequencing technology. A total of 26387032 and 22268804 paired-end reads were obtained after quality filtering for C. amada and Z. officinale, respectively. A total of 36359 and 32312 assembled transcript sequences were obtained from both the species. The functions of the unigenes cover a diverse set of molecular functions and biological processes, among which we identified a large number of genes associated with resistance to stresses and response to biotic stimuli. Large scale expression profiling showed that many of the disease resistance related genes were expressed more in C. amada. Comparative analysis also identified genes belonging to different pathways of plant defense against biotic stresses that are differentially expressed in either ginger or mango ginger. The identification of many defense related genes differentially expressed provides many insights to the resistance mechanism to R. solanacearum and for studying potential pathways involved in responses to pathogen. Also, several candidate genes that may underline the difference in resistance to R. solanacearum between ginger and mango ginger were identified. Finally, we have developed a web resource, ginger transcriptome database, which provides public access to the data. Our study is among the first to demonstrate the use of Illumina short read sequencing for de novo transcriptome assembly and comparison in non-model species of Zingiberaceae.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison of the Transcriptomes of Ginger (Zingiber officinale Rosc.) and Mango Ginger (Curcuma amada Roxb.) in Response to the Bacterial Wilt Infection

et al. 2014

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“…C. amada is a potential donor for bacterial wilt resistance to the susceptible Z. officinale . Three transcripts were discriminative: the expression of genes encoding a leucine-rich protein, a xyloglucan transglycosylase and a GST was much higher in the resistant species ( C. amada ) than in the susceptible species ( Z. officinale ) at every time point studied (Prasath et al, 2013).…”

Section: Gsts In Plant-bacterium Interactionsmentioning

confidence: 99%

Glutathione S-Transferase Enzymes in Plant-Pathogen Interactions

et al. 2018

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Plant glutathione S-transferases (GSTs) are ubiquitous and multifunctional enzymes encoded by large gene families. A characteristic feature of GST genes is their high inducibility by a wide range of stress conditions including biotic stress. Early studies on the role of GSTs in plant biotic stress showed that certain GST genes are specifically up-regulated by microbial infections. Later numerous transcriptome-wide investigations proved that distinct groups of GSTs are markedly induced in the early phase of bacterial, fungal and viral infections. Proteomic investigations also confirmed the accumulation of multiple GST proteins in infected plants. Furthermore, functional studies revealed that overexpression or silencing of specific GSTs can markedly modify disease symptoms and also pathogen multiplication rates. However, very limited information is available about the exact metabolic functions of disease-induced GST isoenzymes and about their endogenous substrates. The already recognized roles of GSTs are the detoxification of toxic substances by their conjugation with glutathione, the attenuation of oxidative stress and the participation in hormone transport. Some GSTs display glutathione peroxidase activity and these GSTs can detoxify toxic lipid hydroperoxides that accumulate during infections. GSTs can also possess ligandin functions and participate in the intracellular transport of auxins. Notably, the expression of multiple GSTs is massively activated by salicylic acid and some GST enzymes were demonstrated to be receptor proteins of salicylic acid. Furthermore, induction of GST genes or elevated GST activities have often been observed in plants treated with beneficial microbes (bacteria and fungi) that induce a systemic resistance response (ISR) to subsequent pathogen infections. Further research is needed to reveal the exact metabolic functions of GST isoenzymes in infected plants and to understand their contribution to disease resistance.

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“…Angiogenesis is the process which plays an important role for the formation of tissue during developmental stages. There are some primary or secondary metabolic compounds found in many plant those could effect the angiogenesis process (Kerbel and Kamen, 2004;Prasath et al, 2013). In study on the extracts of curcuma amada in the fertilized eggs after 15 days of incubation was found that petroleum ether, chloroform extract and methanol extract does not showed any anti-angiogenesis activity thus revealed that blood vessel formation was normal (Sailendra et al, 2010).…”

Section: Mtt Assaymentioning

confidence: 99%

Preliminary Phytochemical Screening and Anti angiogenesis Studies of Different Extracts of Curcuma amada

Tariq¹

2016

Int.J.Curr.Microbiol.App.Sci

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Analysis of differentially expressed genes in Curcuma amada and Zingiber officinale upon infection with Ralstonia solanacearum by suppression subtractive hybridization

Cited by 13 publications

References 35 publications

Comparison of the Transcriptomes of Ginger (Zingiber officinale Rosc.) and Mango Ginger (Curcuma amada Roxb.) in Response to the Bacterial Wilt Infection

Comparison of the Transcriptomes of Ginger (Zingiber officinale Rosc.) and Mango Ginger (Curcuma amada Roxb.) in Response to the Bacterial Wilt Infection

Glutathione S-Transferase Enzymes in Plant-Pathogen Interactions

Preliminary Phytochemical Screening and Anti angiogenesis Studies of Different Extracts of Curcuma amada

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