Objective: the objective of this study was to analyze the characteristics of content posted on Instagram® by periodontists in ColombiaMethods: This study analyzed Instagram accounts of Colombian Periodontists for professional use. A non-probabilistic convenience sample of accounts that met inclusion criteria was selected. Data collection included general information, geographic location, number of followers, number of publications, privacy of the account, profile, and type of publications. Statistical analyses were performed to establish relationships between variables, and the Chi2 test was used to analyze the distribution of categorical variables. Logistic and linear regression analyses were used to determine correlations, and the engagement rate was calculated for the most popular publication. Statistically significant differences were assumed when p≤0.05.Results: 201 Instagram accounts were evaluated. Men had a higher average number of publications than women, and a higher percentage of mixed content. The majority of accounts were business profiles, with the most common type of publication being image/video. Women's posts received more likes and comments than men's, and non-professional publications received more likes on average. For women, reels produced the most interactions. Non-professional content produced the most interactions. The most frequent type of content was professional, while the least frequent was value-oriented content. Women had a higher mean engagement rate (p<0,05). There was a negative overall trend between followers and engagement rate.Conclusions: Colombian periodontists primarily use Instagram for business reasons. However, personal content receives more interactions, and gender appears to have an impact on engagement rate.