Analysis of contribution of protein phosphorylation in the development of the diseases

Zavialova, Maria G.; Zgoda, Victor G.; Nikolaev, Evgeny N.

doi:10.18097/pbmc20176302101

Cited by 6 publications

(3 citation statements)

References 110 publications

(125 reference statements)

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“…Protein phosphorylation plays an important role in the process of cell signal transduction, cell growth and development, and cancer mechanisms (26,27). Numerous studies in cancer cells and tissues resulted in identification of key differences from healthy tissues and cells at the protein level (28). Aberrant regulation of phosphorylation in signaling pathways contributes to carcinogenesis (29).…”

Section: Discussionmentioning

confidence: 99%

FSH receptor binding inhibitor up-regulates ARID1A and PTEN genes associated with ovarian cancers in mice

Gong

Shen

Yang

et al. 2019

Braz J Med Biol Res

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Experiments were conducted to determine if the follicle-stimulating hormone (FSH) receptor binding inhibitor (FRBI) impacts the expression levels of AT-rich interactive domain-containing protein 1A (ARID1A) and phosphatase and tensin homolog (PTEN) in ovaries and blood, as well as expressions of follicle-stimulating hormone cognate receptor (FSHR) gene and proteins. Mice in FRBI-10, FRBI-20, FRBI-30, and FRBI-40 groups were intramuscularly injected with 10, 20, 30, and 40 mg FRBI/kg, respectively, for five consecutive days. Western blotting and qRT-PCR were utilized to determine expression levels of ARID1A and PTEN proteins and mRNAs. Serum ARID1A and PTEN concentrations of the FRBI-40 group were higher than the control group (CG) and FSH group (P<0.05). FSHR mRNA levels of FRBI-20, FRBI-30, and FRBI-40 groups were lower than that of CG and FSH groups on day 15 (P<0.05 or P<0.01). Expression levels of FSHR proteins of FRBI-30 and FRBI-40 groups were lower than those of CG and FSH groups (P<0.05). Levels of ARID1A and PTEN proteins of the FRBI-30 group were greater than CG on days 20 and 30 (P<0.05). FRBI doses had significant positive correlations to levels of ARID1A and PTEN proteins. Additionally, ARID1A and PTEN had negative correlations to FSHR mRNAs and proteins. A high dose of FRBI could promote the expression levels of ARID1A and PTEN proteins in ovarian tissues. FRBI increased serum concentrations of ARID1A and PTEN. However, FRBI depressed expression levels of FSHR mRNAs and proteins in mouse ovaries.

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Section: Discussionmentioning

confidence: 99%

FSH receptor binding inhibitor up-regulates ARID1A and PTEN genes associated with ovarian cancers in mice

Gong

Shen

Yang

et al. 2019

Braz J Med Biol Res

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“…Different proteoforms are separated and quantitatively compared through differences in staining intensity after visualization using highly specific dyes. The fluorescent dye Pro-Q Diamond is typically used for phosphopeptide analysis [ 49 , 50 ]. Nowadays, the more advanced electrophoresis and antibody-based method, Western blotting, is commonly used for phosphoproteomic analysis.…”

Section: Analytical Techniques In Post-translational Modification Analysismentioning

confidence: 99%

Current Methods of Post-Translational Modification Analysis and Their Applications in Blood Cancers

Dunphy

Dowling

Bazou

et al. 2021

Cancers

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Post-translational modifications (PTMs) add a layer of complexity to the proteome through the addition of biochemical moieties to specific residues of proteins, altering their structure, function and/or localization. Mass spectrometry (MS)-based techniques are at the forefront of PTM analysis due to their ability to detect large numbers of modified proteins with a high level of sensitivity and specificity. The low stoichiometry of modified peptides means fractionation and enrichment techniques are often performed prior to MS to improve detection yields. Immuno-based techniques remain popular, with improvements in the quality of commercially available modification-specific antibodies facilitating the detection of modified proteins with high affinity. PTM-focused studies on blood cancers have provided information on altered cellular processes, including cell signaling, apoptosis and transcriptional regulation, that contribute to the malignant phenotype. Furthermore, the mechanism of action of many blood cancer therapies, such as kinase inhibitors, involves inhibiting or modulating protein modifications. Continued optimization of protocols and techniques for PTM analysis in blood cancer will undoubtedly lead to novel insights into mechanisms of malignant transformation, proliferation, and survival, in addition to the identification of novel biomarkers and therapeutic targets. This review discusses techniques used for PTM analysis and their applications in blood cancer research.

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“…При участии специфических ферментов-протеинкиназ и фосфатаз фосфатная группа аденозинтрифосфорной кислоты обратимо присоединяется к аминокислотным остаткам серина, треонина или тирозина β-субъединицы рецептора инсулина, то есть начинается процесс фосфорилирования, который запускает передачу сигнала в клетке. Этот процесс является основным механизмом контроля и регуляции ферментативной активности белков клетки, а также передачи сигнала при различных внеклеточных воздействиях [30].…”

Section: обзоры / Reviewsunclassified

Modern Approaches to Determination of the Biological Activity of Insulin and its

Батуашвили¹,

Симутенко²,

Shadrin³

et al. 2019

Vedomosti Nauchnogo tsentra ekspertizy sredstv meditsinskogo pr

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Федеральное государственное бюджетное учреждение «Научный центр экспертизы средств медицинского применения» Министерства здравоохранения Российской Федерации, Петровский б-р, д. 8, стр. 2, Москва, 127051, Российская Федерация Резюме. Рассмотрены особенности действия инсулина на организм пациента, виды препаратов инсулина и его аналогов, применяемых для лечения сахарного диабета, и предъявляемые к ним требования. Показано, что определение биологической активности является одним из главных показателей качества данной группы препаратов. Приведено краткое описание методов анализа инсулина и его аналогов, основанных как на действии гормона на организм в целом (in vivo: двойной перекрест, эугликемический клэмп), так и на отдельных этапах его взаимодействия с системами организма (in vitro: рецепторно-связывающий анализ, фосфорилирование, метаболические методы). В связи с появлением на фармацевтическом рынке биоаналогов инсулина в статье поднимается вопрос о необходимости сохранения в нормативных документах показателя «Биологическая активность», определяемого на животных. Проведенный анализ методов определения биологической активности in vivo и in vitro убедительно показывает невозможность замены моделей с использованием животных на существующие в настоящее время методы анализа, выполняемые на клеточных культурах. Следовательно, модели на животных по-прежнему необходимы, так как только они позволяют дать адекватную оценку качества инсулинов по показателю «Биологическая активность». Принимая во внимание мировые тенденции к сокращению использования животных в испытаниях, авторы указывают на необходимость разработки методов, результаты которых будут сопоставимы с результатами определения биологической активности in vivo. Ключевые слова: инсулин; аналоги инсулина; биоподобные препараты; биоаналоги; биологическая активность; методы in vivo и in vitro; ИР-1 и ИР-2; рецепторно-связывающий анализ; фосфорилирование; метаболическая активность Для цитирования: Батуашвили ТА, Симутенко ЛВ, Шадрин ПВ, Неугодова НП. Современные подходы к определению биологической активности инсулина и его аналогов. Ведомости Научного центра экспертизы средств медицинского применения. 2019;9(2):85-92. https://doi.Abstract. The paper considers insulin's specific action on the patient's body, types of insulin preparations and insulin analogues which are used for the treatment of diabetes, as well as applicable requirements for these products. It was demonstrated that determination of biological activity is one of the key quality parameters of this type of medicines. The paper summarises the methods used for evaluation of insulin and its analogues, which are based both on the hormone's general action on the body (in vivo: double crossing, euglycemic clamp, etc.), and on certain aspects of the hormone's interaction with the body systems (in vitro: receptor-binding assay, phosphorylation, metabolic methods). Due to the appearance of insulin biosimilars on the pharmaceutical market, the article raises the issue that the «Biological potency» parameter test...

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Analysis of contribution of protein phosphorylation in the development of the diseases

Cited by 6 publications

References 110 publications

FSH receptor binding inhibitor up-regulates ARID1A and PTEN genes associated with ovarian cancers in mice

FSH receptor binding inhibitor up-regulates ARID1A and PTEN genes associated with ovarian cancers in mice

Current Methods of Post-Translational Modification Analysis and Their Applications in Blood Cancers

Modern Approaches to Determination of the Biological Activity of Insulin and its

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