Analysis of CmCADs and three lignifying enzymes in oriental melon (‘CaiHong7’) seedlings in response to three abiotic stresses

Liu, Wei; Jin, Yongfeng; Li, Mengmeng; Dong, Lijuan; Guo, Di; Lu, Chang; Qi, Hongyan

doi:10.1016/j.scienta.2018.04.024

Cited by 24 publications

(22 citation statements)

References 69 publications

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“…As a specific step in the late stages of lignin synthesis, the polymerization of monolignols catalysed by peroxidase (POD) and/or laccase enzymes yields lignin 32 . Previous studies have shown that abiotic stresses generally induce POD activity until it reaches a specific threshold, leading to lignin accumulation 33 , 34 . A previous study also showed that overexpression of POD increases the contents of phenols and lignin in plants 35 .…”

Section: Discussionmentioning

confidence: 99%

Drought stress triggers proteomic changes involving lignin, flavonoids and fatty acids in tea plants

Wang

Xie

et al. 2020

Sci Rep

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Drought stress triggers a series of physiological and biochemical changes in tea plants. It is well known that flavonoids, lignin and long-chain fatty acids play important roles in drought resistance. However, changes in proteins related to these three metabolic pathways in tea plants under drought stress have not been reported. We analysed the proteomic profiles of tea plants by tandem mass tag and liquid chromatography-tandem mass spectrometry. A total of 4789 proteins were identified, of which 11 and 100 showed up- and downregulation, respectively. The proteins related to the biosynthesis of lignin, flavonoids and long-chain fatty acids, including phenylalanine ammonia lyase, cinnamoyl-CoA reductase, peroxidase, chalcone synthase, flavanone 3-hydroxylase, flavonol synthase, acetyl-CoA carboxylase 1,3-ketoacyl-CoA synthase 6 and 3-ketoacyl-CoA reductase 1, were downregulated. However, the contents of soluble proteins, malondialdehyde, total phenols, lignin and flavonoids in the tea plants increased. These results showed that tea plants might improve drought resistance by inhibiting the accumulation of synthases related to lignin, flavonoids and long-chain fatty acids. The proteomic spectrum of tea plants provides a scientific basis for studying the pathways related to lignin, flavonoid and long-chain fatty acid metabolism in response to drought stress.

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Section: Discussionmentioning

confidence: 99%

Drought stress triggers proteomic changes involving lignin, flavonoids and fatty acids in tea plants

Wang

Xie

et al. 2020

Sci Rep

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“…CAD genes usually present as a gene family in different species and each member may function distinctly or redundantly from each other [ 24 , 25 ]. Our team discovered five CmCAD genes in melon genome database ( http://melonomics.cragenomica.es/ ) [ 26 ] and found they can be significantly up-regulated by drought stress in melon seedlings [ 27 ]. However, it remains unclear which signal molecule is responsible for regulating CmCADs under drought stress.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we investigated the regulations of ABA, H 2 O 2 and JA to the five CmCAD genes, as well as some lignin biosynthesis genes, and lignin deposition through using their corresponding inhibitor or scavenger. Then promoter::GUS assays were performed to investigate the regulations of the signal molecules to CmCAD1 , 2 , and 3 , which members were chosen according to the phylogenetic analysis [ 26 ] and the expression profiles under drought stress [ 27 ], as well as the expression patterns in melon seedlings (unpublished data). The results revealed important roles of ABA, H 2 O 2 and JA in regulating CmCAD1~3 , 5 and lignin biosynthesis genes, thus in regulating lignin biosynthesis under drought stress.…”

Section: Introductionmentioning

confidence: 99%

Drought-induced ABA, H2O2 and JA positively regulate CmCAD genes and lignin synthesis in melon stems

et al. 2021

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Background Cinnamyl alcohol dehydrogenase (CAD) is an important enzyme functions at the last step in lignin monomer synthesis pathway. Our previous work found that drought induced the expressions of CmCAD genes and promoted lignin biosynthesis in melon stems. Results Here we studied the effects of abscisic acid (ABA), hydrogen peroxide (H2O2) and jasmonic acid (JA) to CmCADs under drought stress. Results discovered that drought-induced ABA, H2O2 and MeJA were prevented efficiently from increasing in melon stems pretreated with fluridone (Flu, ABA inhibitor), imidazole (Imi, H2O2 scavenger) and ibuprofen (Ibu, JA inhibitor). ABA and H2O2 are involved in the positive regulations to CmCAD1, 2, 3, and 5, and JA is involved in the positive regulations to CmCAD2, 3, and 5. According to the expression profiles of lignin biosynthesis genes, ABA, H2O2 and MeJA all showed positive regulations to CmPAL2-like, CmPOD1-like, CmPOD2-like and CmLAC4-like. In addition, positive regulations were also observed with ABA to CmPAL1-like, CmC4H and CmCOMT, with H2O2 to CmPAL1-like, CmC4H, CmCCR and CmLAC17-like, and with JA to CmCCR, CmCOMT, CmLAC11-like and CmLAC17-like. As expected, the signal molecules positively regulated CAD activity and lignin biosynthesis under drought stress. Promoter::GUS assays not only further confirmed the regulations of the signal molecules to CmCAD1~3, but also revealed the important role of CmCAD3 in lignin synthesis due to the strongest staining of CmCAD3 promoter::GUS. Conclusions CmCADs but CmCAD4 are positively regulated by ABA, H2O2 and JA under drought stress and participate in lignin synthesis.

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“…DEGs associated with this enzyme activity were also more often down- (157) rather than up-regulated (26) across shoot and root (Table 9). A number of previous studies have looked at the activities and/or expression profiles of enzymes from the phenylpropanoid pathway under drought stress and opposite or mixed trends, in terms of their regulation, have been reported [71–74]–though there are considerable variations in the species under study, experimental methodologies and time courses across studies. However, the frequent occurrences of genes involved in the phenylpropanoid pathway in drought studies does indicate the significance of cell-wall metabolism in the response to water-stress.…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression and gene ontologies associated with increasing water-stress in leaf and root transcriptomes of perennial ryegrass (Lolium perenne)

et al. 2019

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Perennial ryegrass ( Lolium perenne ) is a forage and amenity grass species widely cultivated in temperate regions worldwide. As such, perennial ryegrass populations are exposed to a range of environmental conditions and stresses on a seasonal basis and from year to year. One source of potential stress is limitation on water availability. The ability of these perennial grasses to be able to withstand and recover after periods of water limitation or drought can be a key component of grassland performance. Thus, we were interested in looking at changes in patterns of gene expression associated with increasing water stress. Clones of a single genotype of perennial ryegrass were grown under non-flowering growth room conditions in vermiculite supplemented with nutrient solution. Leaf and root tissue was sampled at 4 times in quadruplicate relating to estimated water contents of 35%, 15%, 5% and 1%. RNA was extracted and RNAseq used to generate transcriptome profiles at each sampling point. Transcriptomes were assembled using the published reference genome sequence and differential gene expression analysed using 3 different programmes, DESeq2, edgeR and limma (with the voom transformation), individually and in combination, deriving Early, Middle and Late stage comparisons. Identified differentially expressed genes were then associated with enriched GO terms using BLAST2GO. For the leaf, up-regulated differentially expressed genes were strongly associated with GO terms only during the Early stage and the majority of GO terms were associated with only down-regulated genes at the Middle or Late stages. For the roots, few differentially expressed genes were identified at either Early or Middle stages. Only one replicate at 1% estimated water content produced high quality data for the root, however, this indicated a high level of differential expression. Again the majority of enriched GO terms were associated with down-regulated genes. The performance of the different analysis programmes and the annotations associated with identified differentially expressed genes is discussed.

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Analysis of CmCADs and three lignifying enzymes in oriental melon (‘CaiHong7’) seedlings in response to three abiotic stresses

Cited by 24 publications

References 69 publications

Drought stress triggers proteomic changes involving lignin, flavonoids and fatty acids in tea plants

Drought stress triggers proteomic changes involving lignin, flavonoids and fatty acids in tea plants

Drought-induced ABA, H2O2 and JA positively regulate CmCAD genes and lignin synthesis in melon stems

Differential gene expression and gene ontologies associated with increasing water-stress in leaf and root transcriptomes of perennial ryegrass (Lolium perenne)

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