Analysis of circulating γδ+ T cells in children affected by IgE-associated and non-IgE-associated allergic atopic eczema/dermatitis syndrome

Cairo, Cristiana; Arabito, E.; Landi, Fabiola; Casati, Anna; Brunetti, Ercole; Mancino, Giorgio; Galli, Elena

doi:10.1111/j.1365-2249.2005.02813.x

Cited by 25 publications

(20 citation statements)

References 42 publications

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“…The current report is consistent with previous studies demonstrating changes in the phenotype and function of human γδ T cells in skin inflammation [60,61] and in IBD [11]. The increased expression of skin‐homing molecule CLA on γδ T cells in EN and enhanced gut‐homing receptor CCR9 expression on γδ T cells in CD and UC without cutaneous manifestations may reflect the recruitment of γδ T cells from the circulation to the inflamed skin and gut, respectively.…”

Section: Discussionsupporting

confidence: 92%

Skin- and gut-homing molecules on human circulating γδ T cells and their dysregulation in inflammatory bowel disease

Mann

McCarthy

Peake

et al. 2012

Clinical and Experimental Immunology

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Summary Changes in phenotype and function of γδ T cells have been reported in inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC). Dysregulation of lymphocyte migration plays a key role in IBD pathogenesis; however, data on migratory properties of γδ T cells are scarce. Human circulating γδ T cells from healthy controls (n = 27), patients with active CD (n = 15), active UC (n = 14) or cutaneous manifestations of IBD (n = 2) were characterized by flow cytometry. Circulating γδ T cells in healthy controls were CD3hi and expressed CD45RO. They expressed gut‐homing molecule β7 but not gut‐homing molecule corresponding chemokine receptors (CCR)9, or skin‐homing molecules cutaneous lymphocyte‐associated antigen (CLA) and CCR4, despite conventional T cells containing populations expressing these molecules. CCR9 expression was increased on γδ T cells in CD and UC, while skin‐homing CLA was expressed aberrantly on γδ T cells in patients with cutaneous manifestations of IBD. Lower levels of CD3 expression were found on γδ T cells in CD but not in UC, and a lower proportion of γδ T cells expressed CD45RO in CD and UC. Enhanced expression of gut‐homing molecules on circulating γδ T cells in IBD and skin‐homing molecules in cutaneous manifestations of IBD may be of clinical relevance.

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Section: Discussionsupporting

confidence: 92%

Skin- and gut-homing molecules on human circulating γδ T cells and their dysregulation in inflammatory bowel disease

Mann

McCarthy

Peake

et al. 2012

Clinical and Experimental Immunology

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“…Subsets of κ cells have been found in both mice and humans. The distinct subsets of κ cells with different γ and ® TCR chains that colonize specific epithelial locations in diseases of the lungs, intestines, skin, uterus, vagina and tongue need to be identified 172021222324. As the epithelial cells of nasal mucosa is the first mechanical barrier of the airway, it has a great effect on the mucosal immune system.…”

Section: Discussionmentioning

confidence: 99%

Vγ1⁺γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema

Yang

et al. 2017

Allergy Asthma Immunol Res

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PurposeWe have found that expression of γδT cells is increased in pathological mucosa of chronic rhinosinusitis with nasal polyps (CRSwNP) compared with normal nasal mucosa. This increase is correlated with the infiltration of eosinophils in CRSwNP. Here, we investigated the expression of γδT cells, inflammation and tissue remodeling factors as well as their probable relationships in different types of chronic rhinosinusitis (CRS) in China.MethodsA total of 76 surgical tissue samples that included 43 CRSwNP samples (15 eosinophilic and 28 non-eosinophilic), 17 CRS samples without nasal polyps (CRSsNP), and 16 controls were obtained. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the mRNA expression levels of Vγ1+ γδT cells, Vγ4+ γδT cells, eosinophil cationic protein (ECP), interleukin (IL)-8, transforming growth factor (TGF)-β2, metalloproteinase (MMP)-7, tissue inhibitor of metalloproteinase (TIMP)-4 and hypoxia-inducible factor (HIF)-1α. Enzyme linked immunosorbent assay (ELISA) was used to measure the protein level of ECP and MMP-7 in CRSwNP. The eosinophils were counted and the level of edema was analyzed with HE staining.ResultsThe mRNA expression levels of the Vγ1 subset, ECP and MMP-7 were significantly increased in CRSwNP with histological characteristics of eosinophilic infiltration and edema. The expression of the Vγ1 gene in CRSwNP correlated positively with the expression of both ECP and MMP-7. No significant decreases in the mRNA expression levels of TGF-β2, TIMP-4 or HIF-1α were observed in the CRSwNP samples. The expression levels of Vγ1 gene, ECP and MMP-7 were significantly increased in eosinophilic CRSwNP compared to non-eosinophilic CRSwNP.ConclusionsOur results suggest the associations between Vγ1+ γδT cells, ECP and MMP-7 in CRSwNP, indicating that Vγ1+ γδT cells can induce the eosinophilic inflammation, which has a further effect on the formation of edema.

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“…For instance, Cat's Claw is a traditional South American medicine used to treat a variety of illnesses including allergies, cancer, colitis, dysentery, and urinary tract infection (50). Interestingly, these illnesses are commonly associated with ␥␦ T cell regulatory activities (7,(51)(52)(53)(54), suggesting the described immunostimulatory properties of the Cat's Claw plant could be partially due to the activation of ␥␦ T cells. Preparations containing the apple-condensed tannins we described are also recognized for similar immunostimulatory properties (49,(55)(56)(57).…”

Section: Discussionmentioning

confidence: 99%

Select Plant Tannins Induce IL-2Rα Up-Regulation and Augment Cell Division in γδ T Cells

Holderness

Jackiw

Kimmel

et al. 2007

The Journal of Immunology

118

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γδ T cells are innate immune cells that participate in host responses against many pathogens and cancers. Recently, phosphoantigen-based drugs, capable of expanding γδ T cells in vivo, entered clinical trials with the goal of enhancing innate immune system functions. Potential shortcomings of these drugs include the induction of nonresponsiveness upon repeated use and the expansion of only the Vδ2 subset of human γδ T cells. Vδ1 T cells, the major tissue subset, are unaffected by phosphoantigen agonists. Using FACS-based assays, we screened primary bovine cells for novel γδ T cell agonists with activities not encompassed by the current treatments in an effort to realize the full therapeutic potential of γδ T cells. We identified γδ T cell agonists derived from the condensed tannin fractions of Uncaria tomentosa (Cat’s Claw) and Malus domestica (apple). Based on superior potency, the apple extract was selected for detailed analyses on human cells. The apple extract was a potent agonist for both human Vδ1 and Vδ2 T cells and NK cells. Additionally, the extract greatly enhanced phosphoantigen-induced γδ T cell expansion. Our analyses suggest that a tannin-based drug may complement the phosphoantigen-based drugs, thereby enhancing the therapeutic potential of γδ T cells.

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Analysis of circulating γδ+ T cells in children affected by IgE-associated and non-IgE-associated allergic atopic eczema/dermatitis syndrome

Cited by 25 publications

References 42 publications

Skin- and gut-homing molecules on human circulating γδ T cells and their dysregulation in inflammatory bowel disease

Skin- and gut-homing molecules on human circulating γδ T cells and their dysregulation in inflammatory bowel disease

Vγ1⁺γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema

Select Plant Tannins Induce IL-2Rα Up-Regulation and Augment Cell Division in γδ T Cells

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Analysis of circulating γδ+ T cells in children affected by IgE-associated and non-IgE-associated allergic atopic eczema/dermatitis syndrome

Cited by 25 publications

References 42 publications

Skin- and gut-homing molecules on human circulating γδ T cells and their dysregulation in inflammatory bowel disease

Skin- and gut-homing molecules on human circulating γδ T cells and their dysregulation in inflammatory bowel disease

Vγ1+γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema

Select Plant Tannins Induce IL-2Rα Up-Regulation and Augment Cell Division in γδ T Cells

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Resources

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Vγ1⁺γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema