Analysis of chlorophyll fluorescence parameters as predictors of biomass accumulation and tolerance to heat and drought stress of wheat (

Abstract

Agricultural technologies aimed at increasing yields require the development of highly productive and stress-tolerant cultivars. Phenotyping can significantly accelerate breeding; however, no reliable markers have been identified to select the most promising cultivars at an early stage. In this work, we determined the light-induced dynamic of chlorophyll fluorescence (ChlF) parameters in young seedlings of 10 wheat (Triticum aestivum L.) cultivars and evaluated potency of these parameters as predictors of biomass accumulation and stress tolerance. Dry matter accumulation positively correlated with the effective quantum efficiency of photosystem II (ΦPSIIef) and negatively correlated with the half-time of ΦPSIIef reaching (t1/2(ΦPSIIef)). There was a highly significant correlation between t1/2(ΦPSIIef) and dry matter accumulation with increasing prediction period. Short-term heating and drought caused an inhibition of biomass accumulation and photosynthetic activity depending on the stressor intensity. The positive correlation between the ΦPSII dark level (ΦPSIId) in young seedlings and tolerance to a rapidly increasing short-term stressor (heating) was shown. In the case of a long-term stressor (drought), we revealed a strong negative relationship between tolerance and the level of non-photochemical fluorescence quenching (NPQ). In general, the results show the potency of the ChlF parameters of young seedlings as predictors of biomass accumulation and stress tolerance.

Comparative Study of Photosynthesis Performance of Herbicide-Treated Young Triticale Plants during Drought and Waterlogging Stress

“…This in turn led to a significant decline in F v /F m and F v /F 0 , which is indicating damages to PSII due to water deficit (Figure 4). Similar changes in fluorescence parameters were observed in wheat plants subjected to drought [30,31]. PI total (Figure 5B) utilization of light energy because of dissipation through heat, which is in accordance with earlier reports [32].…”

Physiology, gene expression, and epiphenotype of two Dianthus broteri polyploid cytotypes under temperature stress

“…Although photosynthetic performance was compromised for both cytotypes, particularly after the heat shock, higher photochemical efficiency ( F v / F m and Φ PSII ) and less photoinhibition explain the slightly lower drop in A N for dodecaploids. In line with that, the 12× cytotype also presented greater concentrations of total chlorophylls ( Table 1 ), which may be a consequence or evidence of more robust photosynthesis in this ploidy relative to 4× ( Croft et al , 2017 ) and could reflect higher biomass accumulation ( Sherstneva et al , 2022 ).…”

Prediction of biomass accumulation and tolerance of wheat seedlings to drought and elevated temperatures using hyperspectral imaging

“…F v /F m -maximum quantum yield of photochemical reactions of photosystem II, F PSIIef -steady-state level of photosystem II quantum yield under AL, t 1/2 (F PSIIef ) -time for the F PSII value to reach ½ F PSIIef after switching on AL, NPQ max -maximum value of non-photochemical fluorescence quenching after switching on AL, t(NPQ max ) -time for the NPQ value to reach NPQ max after switching on AL, NPQ s -steady-state NPQ level under AL. Sherstneva et al 10.3389/fpls.2024.1344826 F PSII value to reach ½ F PSIIef after switching on AL (t 1/2 (F PSIIef )), maximum value of non-photochemical fluorescence quenching after switching on AL (NPQ max ), time for the NPQ value to reach NPQ max after switching on AL (t(NPQ max )), and steady-state NPQ level under AL (NPQ s ) (Sherstneva et al, 2021).…”

“…A widely used method for assessing photosynthetic activity is chlorophyll fluorescence (ChlF) recording using Pulse-Amplitude-Modulation (PAM) fluorometry (Maxwell and Johnson, 2000;Baker, 2008;Kalaji et al, 2017;Ni et al, 2019). It is important to note that ChlF parameters determined by the PAM method are effective predictors of economically important traits in wheat under optimal conditions and under the influence of abiotic stress factors (Sharma et al, 2015;Sherstneva et al, 2021;Terletskaya et al, 2021). Our previous work demonstrated the high efficiency of ChlF parameters characterizing transient light-induced processes in the photosynthetic apparatus (PSA) as predictors of biomass accumulation and tolerance to water deficiency and hightemperature stress (Sherstneva et al, 2021).…”