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Plants can be affected by numerous environmental stressors with spatially heterogeneous actions on their bodies. A fast systemic photosynthetic response, which is connected with long-distance electrical signalling, plays an important role in the adaptation of higher plants to the action of stressors. Potentially, measurement of the response by using a photochemical reflectance index (PRI) could be the basis of monitoring photosynthesis under spatially heterogeneous stressors; however, the method has not been previously used for investigating the systemic photosynthetic response. We investigated changes in PRI and photosynthetic parameters (quantum yields of PSI and PSII and nonphotochemical quenching) in intact leaves of pea (Pisum sativum L.) after local heating of another leaf and the propagation of electrical signals through the plant body. We showed that electrical signals decreased the quantum yields of PSI and PSII and increased the nonphotochemical quenching of intact leaves in times ranging from minutes to tens of minutes; the changes were strongly connected with changes in PRI. Additional analysis showed that changes in PRI were caused by an increase of the energy-dependent quenching induced by electrical signals. Thus PRI can be potentially used for monitoring the systemic photosynthetic response connected with long-distance electrical signalling.

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Local damage to plants can induce fast systemic physiological changes through generation and propagation of electrical signals. It is known that electrical signals influence numerous physiological processes including photosynthesis; an increased plant tolerance to actions of stressors is a result of these changes. It is probable that parameters of electrical signals and fast physiological changes induced by these signals can be modified by the long-term actions of stressors; however, this question has been little investigated. Our work was devoted to the investigation of the parameters of burning-induced electrical signals and their influence on photosynthesis under soil water shortage in pea seedlings. We showed that soil water shortage decreased the amplitudes of the burning-induced depolarization signals (variation potential) and the magnitudes of photosynthetic inactivation (decreasing photosynthetic CO2 assimilation and linear electron flow and increasing non-photochemical quenching of the chlorophyll fluorescence and cyclic electron flow around photosystem I) caused by these signals. Moreover, burning-induced hyperpolarization signals (maybe, system potentials) and increased photosynthetic CO2 assimilation could be observed under strong water shortage. It was shown that the electrical signal-induced increase of the leaf stomatal conductance was a potential mechanism for the burning-induced activation of photosynthetic CO2 assimilation under strong water shortage; this mechanism was not crucial for photosynthetic response under control conditions or weak water shortage. Thus, our results show that soil water shortage can strongly modify damage-induced electrical signals and fast physiological responses induced by these signals.

Photosynthesis is an important target of action of numerous environmental factors; in particular, stressors can strongly affect photosynthetic light reactions. Considering relations of photosynthetic light reactions to electron and proton transport, it can be supposed that extremely low frequency magnetic field (ELFMF) may influence these reactions; however, this problem has been weakly investigated. In this paper, we experimentally tested a hypothesis about the potential influence of ELFMF of 18 µT intensity with Schumann resonance frequencies (7.8, 14.3, and 20.8 Hz) on photosynthetic light reactions in wheat and pea seedlings. It was shown that ELFMF decreased non-photochemical quenching in wheat and weakly influenced quantum yield of photosystem II at short-term treatment; in contrast, the changes in potential and effective quantum yields of photosystem II were observed mainly under chronic action of ELFMF. It is interesting that both short-term and chronic treatment decreased the time periods for 50% activation of quantum yield and non-photochemical quenching under illumination. Influence of ELFMF on pea was not observed at both short-term and chronic treatment. Thus, we showed that ELFMF with Schumann resonance frequencies could influence photosynthetic light processes; however, this effect depends on plant species (wheat or pea) and type of treatment (short-term or chronic).