Analysis of checkpoint effect with fork head associated-and ring finger fusion enhanced green fluorescent protein on nasopharyngeal carcinoma 5–8F cells

Abstract

As an oncogene related to mitosis cycle, checkpoint with fork head associated-(FHA) and ring finger (CHFR) participates in cell cycle and nasopharyngeal carcinoma (NPC). Enhanced Green fluorescent protein (EGFP) is a new type of fluorescent probe. In this study, the effect of CHFR fusion enhanced green fluorescent protein (EGFP) on nasopharyngeal carcinoma cells was analyzed. 5–8F human NPC cells were divided into control group, empty vector group (NC) and CHFR group (transfected with CHFR-pEGFP-N1-5–8F cell line). 12 BALB/c-nu mice were divided into control group, empty vector group and CHFR group. The following processes were assessed: cell proliferation, mitosis cycle, expression of CHFR, Aurora A, Cyclin-dependent kinase 2 (CDC2) and Cyclin B1, and tumor volume. Cell proliferation in the CHFR group was significantly lower at 24, 48 and 72 h. The NC and CHFR groups had increased cells in the G0/G1 phase and reduced cells in the S phase 24 h after subculture, without difference between the NC and CHFR groups. The CHFR group had decreased levels of Aurora A, CDC2 and CyclinB1, while tumor volume in 3 groups increased with increased time, and decreased at each time point for the CHFR group compared with other groups (p < 0.05). CHFR was highly expressed in the CHFR tumor group, which was significantly higher than other groups. The CHFR fusion EGFP protein can significantly inhibit Aurora A activity and expression of Aurora A, CDC2 and CyclinB1 by up-regulating the CHFR. The NPC cells were retained in the G0/G1 phase, blocking progression of cell mitosis, and further inhibiting cell proliferation while reducing tumor volume, and inhibiting the tumorigenic ability of NPC cells.