Analysis of changes to lncRNAs and their target mRNAs in murine jejunum after radiation treatment

Abstract

LncRNAs have been reported to play an important role in various diseases. However, their role in the radiation‐induced intestinal injury is unknown. The goal of the present study was to analyse the potential mechanistic role of lncRNAs in the radiation‐induced intestinal injury. Mice were divided into two groups: Control (non‐irradiated) and irradiated. Irradiated mice were administered 14 Gy of abdominal irradiation (ABI) and were assessed 3.5 days after irradiation. Changes to the jejuna of ABI mice were analysed using RNA‐Seq for alterations to both lncRNA and mRNA. These results were validated using qRT‐PCR. LncRNAs targets were predicted based on analysis of lncRNAs‐miRNAs‐mRNAs interaction. 29 007 lncRNAs and 17 142 mRNAs were detected in the two groups. At 3.5 days post‐irradiation, 91 lncRNAs and 57 lncRNAs were significantly up‐ and downregulated respectively. Similarly, 752 mRNAs and 400 mRNAs were significantly up‐ and downregulated respectively. qRT‐PCR was used to verify the altered expression of four lncRNAs (ENSMUST00000173070, AK157361, AK083183, AK038898) and four mRNAs (Mboat1, Nek10, Ccl24, Cyp2c55). Gene ontology and KEGG pathway analyses indicated the predicted genes were mainly involved in the VEGF signalling pathway. This study reveals that the expression of lncRNAs was altered in the jejuna of mice post‐irradiation. Moreover, it provides a resource for the study of lncRNAs in the radiation‐induced intestinal injury.

The Differential Expression of Long Noncoding RNAs in Type 2 Diabetes Mellitus and Latent Autoimmune Diabetes in Adults

“…Initially, lncRNAs were viewed as "junk genes" that lack coding capacity [9]; however, they were discovered to be important in many human diseases such as diabetes, cancer, neurodegenerative diseases, cardiovascular diseases, and autoimmune diseases, such as T1DM [10,11]. LncRNAs can interact with DNA, proteins, mRNAs, miRNAs, siRNAs, and other antisense RNAs via chromatin modification [8,12], thereby acting as a bait or promoter of transcription factors [4], DNA methylation, histone modification, and genomic imprinting [13] and promoting or inhibiting translation; these interactions are involved in gene expression and regulation [14].…”

Genome-Wide Analysis of Long Noncoding RNAs in Porcine Intestine during Weaning Stress

“…CD patients reportedly have eight DElncRNAs (four up- and four downregulated) in pathological mucosa from the ileal end [ 31 ]. Radiation-induced intestinal damage reveals that 91 lncRNAs are significantly upregulated and 57 lncRNAs are downregulated in murine jejunum [ 32 ]. Infection with porcine endemic diarrhea virus induces lncRNA differential expression in piglet ileum; lncRNA_9606 is significantly upregulated 300-fold [ 33 ].…”

Aberrantly expressed long non‐coding RNAs in air pollution‐induced congenital defects

“…Gene Ontology annotations and GO terms were merit prognosis for function and trend of these genes. Kyoto Encyclopedia of Genes and Genomes signal pathway is another effective tool that can give power information for function of genes and was largely used for bioinformatic analysis . Thus, we determined the lncRNA‐dependent gene functions and the association of correlated signalling pathways in pathogenesis of air pollution‐induced congenital malformation using these two methods.…”

“…Kyoto Encyclopedia of Genes and Genomes signal pathway is another effective tool that can give power information for function of genes and was largely used for bioinformatic analysis. 43,44 Thus, we determined the ln-cRNA-dependent gene functions and the association of correlated signalling pathways in pathogenesis of air pollution-induced congenital malformation using these two methods. Our results demonstrated the possible involvements of neurological system process, sensory perception of smell and G-protein signalling pathway.…”