Analysis of Change Patterns of Microcomputed Tomography 3-Dimensional Bone Parameters as a High-Throughput Tool to Evaluate Antiosteoporotic Effects of Agents at an Early Stage of Ovariectomy-Induced Osteoporosis in Mice

Xiang, Anbo; Kanematsu, Masahiro; Mitamura, Mana; Kikkawa, Hideo; Asano, Shigetaka; Kinoshita, Mine

doi:10.1097/01.rli.0000236921.77810.56

Cited by 22 publications

(15 citation statements)

References 37 publications

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“…20 Xiang et al reported that the trabecular bone volume, thickness, number, and connectivity of distal metaphyseal femur bones significantly decreased 2 weeks post-OVX in mice. 21 Our results also indicated statistical changes in the BV/TV of the L4 vertebral bone, suggesting the beginning of bone loss, in agreement with previous reports. In this study, the tendency toward bone loss was corrected only at 2 weeks after OVX with OA treatment, owing to the slight statistical significance exhibited by these indices between the sham and OVX rats.…”

Section: Discussionsupporting

confidence: 94%

Oleanolic acid exerts an osteoprotective effect in ovariectomy-induced osteoporotic rats and stimulates the osteoblastic differentiation of bone mesenchymal stem cells in vitro

et al. 2012

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Section: Discussionsupporting

confidence: 94%

Oleanolic acid exerts an osteoprotective effect in ovariectomy-induced osteoporotic rats and stimulates the osteoblastic differentiation of bone mesenchymal stem cells in vitro

et al. 2012

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“…In recent years, micro-computerized tomography (μCT) has been used to measure 3D bone structure of animals due to its relative ease in sample preparation, its noninvasive nature, and the high spatial resolution as compared with conventional histology [11]. Studies have demonstrated that μCT analysis of change patterns in 3D bone parameters may serve as a valuable tool for the evaluation of both anti-resorptive and anabolic agents in ovariectomized (OVX) animals [12]. …”

Section: Introductionmentioning

confidence: 99%

Microarchitecture and Nanomechanical Properties of Trabecular Bone After Strontium Administration in Osteoporotic Goats

Peng

Pan

et al. 2011

Biol Trace Elem Res

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Strontium (Sr) ralenate is a new agent used for the prevention and treatment of osteoporosis. As a bone-seeking element, 98% of Sr is deposited in the bone and teeth after oral ingestion. However, the effect of Sr treatment on bone microarchitecture and bone nanomechanical properties remains unclear. In this study, 18 osteoporotic goats were divided into four groups according to the treatment regimen: control, calcium alone (Ca), calcium and Sr at 24 mg/kg (Ca + 24Sr), and calcium and Sr at 40 mg/kg (Ca + 40Sr). The effects of Sr administration on bone microarchitecture and nanomechanical properties of trabecular bones were analyzed with micro-CT and nanoindentation test, respectively. Serum Sr levels increased six- and tenfold in the Ca + 24Sr and Ca + 40Sr groups, respectively. Similarly, Sr in the bone increased four- and sixfold in these two groups. Sr administration significantly increased trabecular bone volume fraction, trabecular thickness, and double-labeled new bone area. Sr administration, however, did not significantly change the nanomechanical properties of trabecular bone (elastic modulus and hardness). The data suggested that Sr administration increased trabecular bone volume and improved the microarchitecture while maintaining the intrinsic tissue properties in the osteoporotic goat model.

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“…The discrepancies seen in the histomorphometry parameters between ovariectomized and sham-operated groups were surprising and it may be attributed to the small sample size used in our study. In addition, the technique used in measuring the histomorphometric parameters may not be sensitive since others have found that the use of high-throughput technique such as microcomputed tomography was able to yield more significant results [25]. …”

Section: Discussionmentioning

confidence: 99%

The effects of Cosmos caudatus (ulam raja) on dynamic and cellular bone histomorphometry in ovariectomized rats

Mohamed

Sahhugi²,

Ramli

et al. 2013

BMC Res Notes

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BackgroundCosmos caudatus is a local plant which has antioxidant properties and contains high calcium. It is also reported to be able to strengthen the bone. This report is an extension to previously published article in Evidence Based Complementary and Alternative Medicine (doi:10.1155/2012/817814). In this study, we determined the effectiveness of C. caudatus as an alternative treatment for osteoporosis due to post-menopause by looking at the dynamic and cellular paramaters of bone histomorphometry.MethodsForty female Wistar rats were divided into four groups i.e. sham operated, ovariectomized, ovariectomized treated with calcium 1% ad libitum and ovariectomized force-fed with 500 mg/kg C. caudatus extract. Treatment was given six days a week for eight weeks.ResultsDynamic and cellular histomorphometry parameters were measured. C. caudatus increased double-labeled surface (dLS/BS), mineral appositional rate (MAR), osteoid volume (OV/BV) and osteoblast surface (Ob.S/BS). C. caudatus also gave better results compared to calcium 1% in the osteoid volume (OV/BV) parameter.ConclusionsC. caudatus at the 500 mg/kg dose may be an alternative treatment in restoring bone damage that may occur in post-menopausal women.

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Analysis of Change Patterns of Microcomputed Tomography 3-Dimensional Bone Parameters as a High-Throughput Tool to Evaluate Antiosteoporotic Effects of Agents at an Early Stage of Ovariectomy-Induced Osteoporosis in Mice

Cited by 22 publications

References 37 publications

Oleanolic acid exerts an osteoprotective effect in ovariectomy-induced osteoporotic rats and stimulates the osteoblastic differentiation of bone mesenchymal stem cells in vitro

Oleanolic acid exerts an osteoprotective effect in ovariectomy-induced osteoporotic rats and stimulates the osteoblastic differentiation of bone mesenchymal stem cells in vitro

Microarchitecture and Nanomechanical Properties of Trabecular Bone After Strontium Administration in Osteoporotic Goats

The effects of Cosmos caudatus (ulam raja) on dynamic and cellular bone histomorphometry in ovariectomized rats

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