Analysis of cerium oxide and copper oxide nanoparticles bioaccessibility from radish using SP‐ICP‐MS

Abstract

BACKGROUND The transformation of nanoparticles (NPs) internalized in plant tissues is the human digestive system that can provide a better understanding of the impact of NPs on the human system. The presented methodology was developed to study the bioaccessibility of cerium oxide (CeO2) and copper oxide (CuO) NPs from radish after the in vitro simulation of gastrointestinal digestion using single‐particle inductively coupled plasma mass spectrometry (SP‐ICP‐MS). RESULTS Radish plants were cultivated hydroponically in a growth medium containing: (i) CeO2 NPs and (ii) CuO NPs. Both cerium (Ce) and copper (Cu) were found in all organs of the radish plants after analysis by standalone ICP‐MS. This confirms the bioaccumulation of CeO2 and CuO NPs and the translocation of their Ce and Cu to the aerial parts of the plant. Less Ce (4.095 μg g−1) has been detected in leaves than in roots (1.156 mg g−1) while Cu content in leaves was 5.245 μg g−1 and in roots was 10.41 μg g−1. Analysis of the digestive extracts obtained after the in vitro simulation of gastro (pepsin) and gastrointestinal (pancreatin) digestion showed that Ce has easy access to human system at least by 73%. CONCLUSION The size of CeO2 NPs in digestive extracts showed no significant changes. However, the results obtained for CuO NPs digestion were variable and suggested that CuO NPs dissolved during the digestion process. The CuO NPs were observed in roots after the gastrointestinal digestion concluding that CuO NPs recovered after the initial dissolution. © 2020 Society of Chemical Industry

Uptake and Translocation of Mesoporous SiO2-Coated ZnO Nanoparticles to Solanum lycopersicum Following Foliar Application

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Analytical applications of single particle inductively coupled plasma mass spectrometry: a comprehensive and critical review

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Analytical Methodologies for Agrometallomics: A Critical Review

“…Besides, SP-ICP-MS also contributes to the risk assessment of microparticles in agricultural lives, e.g., the transformation investigation of nanoparticles internalized in plants and animals by SP-ICP-MS can provide a better understanding of nanomaterials' impact on the human system after ingestion. 280 Additionally, silver, gold, carbon, etc. nanoparticles and microplastic materials all can be measured by SP-ICP-MS for their size, distribution, and particle concentration in various sample matrices to reveal the potential health and ecological risks.…”

“…For example, SP-ICP-MS was employed to characterize and quantify engineered nanoparticles in water and at a water treatment facility to alleviate nanoparticle pollution. Besides, SP-ICP-MS also contributes to the risk assessment of microparticles in agricultural lives, e.g., the transformation investigation of nanoparticles internalized in plants and animals by SP-ICP-MS can provide a better understanding of nanomaterials’ impact on the human system after ingestion . Additionally, silver, gold, carbon, etc.…”