Analysis of cephalosporins by hydrophilic interaction chromatography

Liu, Qiaoxia; Xu, Lingyan; Ke, Yanxiong; Jin, Yu; Zhang, Feifang; Liang, Xinmiao

doi:10.1016/j.jpba.2010.09.040

Cited by 26 publications

(4 citation statements)

References 25 publications

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“…These HPLC methods required the use of an ion-pairing agent for CFX content determination according to the European Pharmacopeia related substances monograph of CFX sodium [9]. Recently, separation of cephalosporin was attempted by HILIC [10], but this approach was not suitable for the analysis of degradation products and quantitative determination of CFX. HPLC without ion pairing provided rapid analysis [11].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Ceftriaxone Stability at New-bornsÃ¢ÂÂ Rectal PH Assessed by UV and HPLC Methods

Gaudin

Langlois²,

Kauss³

et al. 2015

Pharm Anal Acta

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This study showed the comparison of UV spectroscopy and High Performance Liquid Chromatography (HPLC) for ceftriaxone stability. UV spectroscopy using wavelength ratio between 241 and 271 nm absorbance values can be used successfully as a screening technique in ceftriaxone stability investigations. Ion paring reversed phase-High Performance Liquid Chromatography provided more precise stability characterization. The HPLC conditions developed were in isocratic mode using an YMC ODS H80, 150 x 4.6 mm, 4 µm with a mobile phase composed by 40% of methanol and 60% of phosphate buffer (10 mM; pH 7.5) where tetrabutylammonium bromide was solubilized at 18 mM. Detection was performed with a diode array detector from 200 to 400 nm. Sample injection volume was at 5 µL. Methanol was selected because better symmetry of ceftriaxone peak than acetonitrile was obtained. Both methods were validated. The calibration curve and stability study was performed over a concentration range of 7.5 to 16.5 mg.L-1. 100% corresponded to the concentration of 15 mg.L-1. Intermediate precision was tested on 6 independent samples at concentrations corresponding to 100% (15 mg.L-1) on 6 consecutive days. These values were within the acceptance criteria of 2% and showed that both methods were precise. Accuracy of the method was evaluated analyzing three independent samples at concentrations corresponding to 100%. Recovery percentage calculated between the known concentration and the calculated concentration of ceftriaxone showed that the methods were accurate. Thus both methods were linear. The stability study was performed at 40°C as infants with sepsis are generally febrile. Over the rectal pH range recorded in sick infants, the stability of ceftriaxone was maximal at pH 7.5. Over 6 hours in a pH range of 6.5 to 8.5 less than 10% of ceftriaxone is degraded. However at pH 5.5, degradation occurred more rapidly and loss of drug was significant.

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Section: Introductionmentioning

confidence: 99%

In Vitro Ceftriaxone Stability at New-bornsÃ¢ÂÂ Rectal PH Assessed by UV and HPLC Methods

Gaudin

Langlois²,

Kauss³

et al. 2015

Pharm Anal Acta

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“…Several methods have been reported for the determination of CFX, including spectrophotometry [43][44][45][46][47] and chromatography. [48][49][50][51][52] Though these methods give good sensitivity and selectivity they have disadvantages, including high costs, long analysis times, and the requirement of complex and tedious sample pretreatments. On the other hand, electrochemical methods have attracted great interest because of their simplicity, rapidness and high sensitivity in detecting CFX without requiring tedious pretreatments.…”

Section: Introductionmentioning

confidence: 99%

Construction of an electrochemical sensor based on the electrodeposition of Au–Pt nanoparticles mixtures on multi-walled carbon nanotubes film for voltammetric determination of cefotaxime

Shahrokhian

Rastgar

2012

Analyst

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Mixtures of gold-platinum nanoparticles (Au-PtNPs) are fabricated consecutively on a multi-walled carbon nanotubes (MWNT) coated glassy carbon electrode (GCE) by the electrodeposition method. The surface morphology and nature of the hybrid film (Au-PtNPs/MWCNT) deposited on glassy carbon electrodes is characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. The modified electrode is used as a new and sensitive electrochemical sensor for the voltammetric determination of cefotaxime (CFX). The electrochemical behavior of CFX is investigated on the surface of the modified electrode using linear sweep voltammetry (LSV). The results of voltammetric studies exhibited a considerable improvement in the oxidation peak current of CFX compared to glassy carbon electrodes individually coated with MWCNT or Au-PtNPs. Under the optimized conditions, the modified electrode showed a wide linear dynamic range of 0.004-10.0 μM with a detection limit of 1.0 nM for the voltammetric determination of CFX. The modified electrode was successfully applied for the accurate determination of trace amounts of CFX in pharmaceutical and clinical preparations.

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“…In the references several methods of analysis for ceftriaxone sodium were found. These methods are high performance liquid chromatography (HPLC) [ 15 , 16 , 17 , 18 ] fluorimetry [ 19 , 20 ], titration [ 21 ], spectrophotometric [ 21 , 22 , 23 , 24 , 25 ] and micellar electrokinetic capillary chromatography [ 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Successful Microbiological Agar Assay for Determination of Ceftriaxone Sodium in Powder for Injectable Solution

Aléssio

Salgado

2012

Pharmaceutics

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Ceftriaxone sodium is a cephalosporin with broad-spectrum antimicrobial activity and belongs to the third generation of cephalosporins. Regarding the quality control of medicines, a validated microbiological assay for the determination of ceftriaxone sodium in powder for injectable solution has not been reported yet. This paper reports the development and validation of a simple, accurate and reproducible agar diffusion method to quantify ceftriaxone sodium in powder for injectable solution. The assay is based on the inhibitory effect of ceftriaxone sodium on the strain of Bacillus subtilis ATCC 9371 IAL 1027 used as test microorganism. The results were treated statistically by analysis of variance and were found to be linear ( r = 0.999) in the selected range of 15.0–60.0 μg/mL, precise with a relative standard deviation (RSD) of repeatability intraday = 1.40%, accurate (100.46%) and robust with a RSD lower than 1.28%. The results demonstrated the validity of the proposed bioassay, which allows reliable ceftriaxone sodium quantitation in pharmaceutical samples and therefore can be used as a useful alternative methodology for the routine quality control of this medicine.

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Analysis of cephalosporins by hydrophilic interaction chromatography

Cited by 26 publications

References 25 publications

In Vitro Ceftriaxone Stability at New-bornsÃ¢ÂÂ Rectal PH Assessed by UV and HPLC Methods

In Vitro Ceftriaxone Stability at New-bornsÃ¢ÂÂ Rectal PH Assessed by UV and HPLC Methods

Construction of an electrochemical sensor based on the electrodeposition of Au–Pt nanoparticles mixtures on multi-walled carbon nanotubes film for voltammetric determination of cefotaxime

Development and Validation of a Successful Microbiological Agar Assay for Determination of Ceftriaxone Sodium in Powder for Injectable Solution

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Analysis of cephalosporins by hydrophilic interaction chromatography

Cited by 26 publications

References 25 publications

In Vitro Ceftriaxone Stability at New-bornsÃ¢ÂÂ Rectal PH Assessed by UV and HPLC Methods

In Vitro Ceftriaxone Stability at New-bornsÃ¢ÂÂ Rectal PH Assessed by UV and HPLC Methods

Construction of an electrochemical sensor based on the electrodeposition of Au–Pt nanoparticles mixtures on multi-walled carbon nanotubes film for voltammetric determination of cefotaxime

Development and Validation of a Successful Microbiological Agar Assay for Determination of Ceftriaxone Sodium in Powder for Injectable Solution

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In Vitro Ceftriaxone Stability at New-bornsÃ¢ÂÂ Rectal PH Assessed by UV and HPLC Methods

In Vitro Ceftriaxone Stability at New-bornsÃ¢ÂÂ Rectal PH Assessed by UV and HPLC Methods