Analysis of cephalosporin antibiotics

El-Shaboury, Salwa R.; Saleh, Gamal A.; Mohamed, Fardous A.; Rageh, Azza H.

doi:10.1016/j.jpba.2007.06.002

Cited by 116 publications

(75 citation statements)

References 228 publications

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“…This bactericide belongs to the group of cephalosporins and can be effective both for Gram positive and Gram negative bacteria. Despite the positive action in the control of bacteria during in vitro cultivation (El-Shaboury, Saleh, Mohamed, & Rageh, 2007), in this study the bactericidal potential of cefaclor negatively affected the development of banana explants when added to growth medium in high concentrations.…”

Section: Antibioticsmentioning

confidence: 64%

<b>Biochemical characterization of systemic bacteria in bananas, sensitivity to antibiotics and plant phytotoxicity during shoot proliferation

Oliveira

Scherwinski‐Pereira

2016

Acta Sci. Agron.

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ABSTRACT. The objective of this work was to characterize the biochemically systemic bacterial isolated from banana plants, to evaluate the bacterial sensitivity to antibiotics, and to determine the phytotoxicity of banana shoots during in vitro proliferation. Systemic bacteria belonging to the Klebsiella and Aeromonas genera were isolated from the "Maravilha" (FHIA 01 AAAB), "Preciosa" (PV 4285 AAAB) and "Thap Maeo" (AAB) varieties and were then characterized. Tests of shoot sensitivity to antibiotics were performed, and the minimum inhibitory concentration (MIC) and phytotoxic effects of selected antibiotics to plants were determined. Among the 20 antibiotics evaluated, the strains showed sensitivity to cefaclor, cefalexin, cefalotin, nalidixic acid, chloramphenicol, and vancomycin. However, during MIC determination, the best results were obtained with cefaclor, vancomycin or nalidixic acid alone in concentrations ranging from 512 to 1,024 mg L -1 . In culture medium, cefaclor at 1,024 mg L -1 was the only antibiotic to affect the multiplication and the shoot survival in culture.Keywords: Musa spp., micropropagation, contamination, endophytic microorganisms, antimicrobial control.Caracterização bioquímica de bactérias sistêmicas em bananeiras, sensibilidade a antibióticos e fitotoxicidade de plantas durante a proliferação de brotos RESUMO. O objetivo do trabalho foi caracterizar bioquimicamente bactérias sistêmicas isoladas de plantas de bananeiras, avaliar a sensibilidade das bactérias a antibióticos e determinar a fitotoxicidade de brotos de bananeiras durante a proliferação in vitro. Bactérias sistêmicas pertencentes aos gêneros Klebsiella e Aeromonas foram isoladas a partir das variedades "Maravilha" (FHIA 01 AAAB), "Preciosa" (PV 4285 AAAB) e "Thap Maeo" (AAB), sendo em seguida caracterizadas. Testes de sensibilidade das brotações aos antibióticos foram desenvolvidos e a mínima concentração inibitória (MIC) e os efeitos fitotóxicos dos antibióticos selecionados em relação aos brotos foram determinados. Entre os 20 antibióticos avaliados, verificou-se que as bactérias mostraram sensibilidade para o cefaclor, cefalexina, cefalotina, ácido nalidíxico, cloranfenicol e vancomicina. Entretanto, durante a determinação da MIC os melhores resultados foram obtidos com cefaclor, vancomicina e ácido nalidixico em concentrações entre 512 a 1.024 mg L -1 . Em meio de cultura, o cefaclor na concentração de 1.024 mg L -1 foi o único a afetar a multiplicação e a sobrevivência de brotos em cultivo.Palavras-chave: Musa spp., micropropagação, contaminação, microrganismos endofíticos, controle microbiano.

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Section: Antibioticsmentioning

confidence: 64%

<b>Biochemical characterization of systemic bacteria in bananas, sensitivity to antibiotics and plant phytotoxicity during shoot proliferation

Oliveira

Scherwinski‐Pereira

2016

Acta Sci. Agron.

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“…It stands out because CFZ has activity against some species of Enterobacter and can be administered less frequently because of its longer half-life. [1][2][3][4][5] Its mechanism of action results from the inhibition of cell wall synthesis in Gram-negative and Gram-positive bacteria, preventing transpeptidases enzymes to form cross-links peptidoglycan in the bacterial cell wall. [6][7][8][9] Considering the great importance of cefazolin sodium on the global scene in the treatment of infectious diseases, the development of practical, economical and reliable analytical methods, which can be used in the quality control of this drug, is essential and highly relevant, seeking the therapeutic efficacy, patient's safety and also benefits for the pharmaceutical industries and compounding pharmacies.…”

Section: Introductionmentioning

confidence: 99%

New Environmentally Friendly Method for Quantification of Cefazolin Sodium

Rechelo¹,

Fernandes²,

Kogawa³

et al. 2017

ECB

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Cefazolin sodium, a β-lactam antimicrobial agent belonging to the first generation cephalosporins, has a broad spectrum of action, acting against gram positive and gram negative bacteria. Stands out over other cephalosporins for its ability to also act against some species of Enterobacter, and have a long half-life, thus reducing the frequency of administrations. A simple, fast and reproducible method by visible spectrophotometry was developed and validated for quantification of cefazolin sodium in the lyophilized powder. This technique is widely used in the pharmaceutical industry due to its ease of execution, low cost, safety and high precision and accuracy. It has been employed in the quality control routine of numerous pharmaceuticals in order to identify them and quantify their active principles. The method was capable of detecting and quantifying the cefazolin sodium obtaining satisfactory results regarding selectivity, precision, accuracy and robustness, on linear range of 32.0 to 92.0 µg mL -1 , showing the correlation coefficient of 0.9993 when analyzed at 767 nm. Due to the environmental impacts caused by global economic development, green chemistry has come up with a proposal to minimize and/or eliminate the use of harmful solvents, which generate large amounts of toxic waste to the environment and the health of operators, as well as reducing expenses with costly processes. The proposed method does not use toxic solvent, proving to be effective, low cost, easy to apply and safe for the analyst and environment. *Corresponding AuthorsFax: +55 16 3301 6967 E-Mail: ac_kogawa@yahoo.com.br [a]

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“…Although high performance liquid chromatography (HPLC) remains by far the most frequently used method in the analysis of cephalosporins, capillary electrophoresis (CE) is being increasingly employed due to its favorable characteristics (high separation efficiency, large flexibility, rapid method development and low consumption of samples and reagents). [6][7][8] Separating simultaneously different types of cephalosporins from different generations remains a challenging task for HPLC, but the resolving power of CE can make this separation relatively fast and simple. 6 Cephalosporins are analyzed usually through two different electrophoretic techniques: capillary zone electrophoresis (CZE) [9][10][11][12][13][14][15] used for the separation of ionic or ionogenic cephalosporins when separation is based on the differences between the electrophoretic mobilities of the analytes; and micellar electrokinetic chromatography (MEKC) [16][17][18][19] used for the separation of both neutral and/or ionic or ionogenic cephalosporins when separation is based on the generation of a pseudostationary phase in which analyte partition takes place.…”

Section: Introductionmentioning

confidence: 99%

“…[6][7][8] Separating simultaneously different types of cephalosporins from different generations remains a challenging task for HPLC, but the resolving power of CE can make this separation relatively fast and simple. 6 Cephalosporins are analyzed usually through two different electrophoretic techniques: capillary zone electrophoresis (CZE) [9][10][11][12][13][14][15] used for the separation of ionic or ionogenic cephalosporins when separation is based on the differences between the electrophoretic mobilities of the analytes; and micellar electrokinetic chromatography (MEKC) [16][17][18][19] used for the separation of both neutral and/or ionic or ionogenic cephalosporins when separation is based on the generation of a pseudostationary phase in which analyte partition takes place. CE has been successfully applied for the analysis of cephalosporins from different pharmaceutical forms, 15 environmental samples, 14 plasma, 9 blood serum, 20 urine and bile, 10 bronchial secretion, 13 but also in stability studies 12 and for the determination of dissociation constants.…”

Section: Introductionmentioning

confidence: 99%