Analysis of cellular fatty acids and proteins by capillary gas chromatography and sodium dodecyl sulphate polyacrylamide gel electrophoresis to differentiate Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum (MAIS) complex species

Identification and subspecific differentiation of Mycobacterium scrofulaceum by automated sequencing of a region of the gene (hsp65) encoding a 65-kilodalton heat shock protein

“…Furthermore, variation in phenotypic characteristics of atypical mycobacterial species makes the unambiguous interpretation of biochemical test results difficult. Several strategies formulated to improve the conventional methods of mycobacterial strain identification include the use of commercial nucleic acid probe kits (34), analysis of cell wall lipid composition by various chromatographic methods (5,7,11,52,53,55), restriction fragment length polymorphism profiling of a 360-bp fragment of the hsp65 gene (51), and nucleotide sequencing of the gene encoding 16S rRNA (1,28,41,42,48). Each of these techniques has several advantages and disadvantages.…”

“…Mycobacterial serovars 27 and 41 through 43 are generally recognized as being M. scrofulaceum; however, serovar designations do not always correspond to the species assignment (15,59). Additional strategies developed for species designation of mycobacteria include analysis of cell wall lipid composition by thin-layer chromatography (5,55), gas-liquid chromatography (11,45,53), and high-performance liquid chromatography (7,52); restriction fragment length polymorphism profiling of a 360-bp fragment of the hsp65 gene (51); and nucleotide sequencing of several gene targets (12,13,46,47,49), primarily the gene encoding 16S rRNA (16S rDNA) (1,28,41,42,48).…”

Non‐tuberculous mycobacterial skin infections

“…Gas chromatography has been used to investigate on mycolic acids and a secondary alcohol was found which is a discriminating constituent between M. scrofulaceum and the other two species. The lipidic analysis is not able to separate M. avium and M. intracellulare, so cell proteins must be considered; it may be a possible to obtain three different patterns using sodium dodecyl sulphate poly aery lamide gel electrophoresis [120]. Another case involved a 50-year-old male patient with a hemoptysis lasting for several months without an identified cause.…”