Analysis of Cannabinoids in Lotions Using High-Performance Liquid Chromatography

Quiñones, Rosalynn; Casiano-Negroni, Anette; Pijor, Haley; Moreno, Sara; Suarez, Karla; Westfall, Tamara D.; Sullins, Carrie; Ivey, Sarah; Buxó, José A.

doi:10.1021/acs.jchemed.2c00214

Cited by 8 publications

(14 citation statements)

References 18 publications

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“…Cannabinoids' increased prevalence in consumer products makes them ideal analytes to facilitate student engagement. 29 From their benefit as medical treatments 30,31 to their legalization for recreational use, public interest in cannabis spans diverse student backgrounds and majors. 32 Both cannabidiol (CBD) and tetrahydrocannabinoid (THC) are linked to various topics in pharmacology, toxicology, and forensics.…”

Section: Hplc Background: Cannabinoidsmentioning

confidence: 99%

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Uncovering the Importance of Peer-to-Peer Communication during the COVID-19 Pandemic: Rotating Chromatography Experiments to Overcome Limited Student Capacity

et al. 2023

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Even though scientific communication and collaboration play critical roles in academic success, they often come in second to teaching laboratory fundamentals. COVID-19 associated capacity constraints forced our program to reexamine the traditional laboratory instruction paradigm by limiting physical attendance, as well as laboratory duration. Instead, we opted to turn these restrictions into opportunities to study peer-to-peer communication as a means to enhance in-person experimentation. Here, a two-week high performance liquid and gas chromatography (HPLC and GC) module uses centralized student communication between peer groups to not only maintain but increase quality laboratory experiences. Students rotate between two chromatography experiments to ensure each person gains exposure to foundational separations techniques. The module’s first week focuses on method development, while the second week highlights method validation. Since HPLC and GC can accommodate similar experimental objectives, students receive comparable method development and validation experiences no matter which specific instrument they are assigned to that week. By integrating communication into the experimental process and adding peer accountability to the laboratory’s design, the authors observed an increase in laboratory report words and figures when compared to the prior years’ reports. Student questionnaires also indicated structured rotation and group communication increased student interest and investment in obtaining and reporting quality data.

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Section: Hplc Background: Cannabinoidsmentioning

confidence: 99%

“…Cannabinoids’ increased prevalence in consumer products makes them ideal analytes to facilitate student engagement . From their benefit as medical treatments , to their legalization for recreational use, public interest in cannabis spans diverse student backgrounds and majors .…”

Section: Introductionmentioning

confidence: 99%

Uncovering the Importance of Peer-to-Peer Communication during the COVID-19 Pandemic: Rotating Chromatography Experiments to Overcome Limited Student Capacity

et al. 2023

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“…30,31 The paper "Analysis of Cannabinoids in Lotions Using High-Performance Liquid Chromatography" by Quinõnes et al applies similar concepts, but HPLC was utilized for separation and students went further by creating a calibration curve to allow quantification of cannabinoids present in commercial lotions. 32 In the paper "Chemistry of Cannabis: An Interdisciplinary Course", Johnston describes the successful development and implementation of a course dedicated to cannabis chemistry. 33 There is therefore widespread interest in utilizing commercial product analysis and the chemistry of cannabis in modern chemistry curricula.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Several experiments utilizing GC–MS to obtain industrial, forensic, and/or environmentally significant data have been successfully implemented by educators to teach undergraduate students about this powerful instrument. Many other experiments have also been published that involve GC–MS analysis of commercial products, such as flavored rapeseed oil, volatile compounds used as fragrance and flavor additives, fatty acids present in commercial cooking oils and fats, caffeine in commercial beverages, xylitol in chewing gum, pesticides extracted from commercial plant foods, hemp fibers, and, perhaps most often, essential oils. , The paper “Analysis of Cannabinoids in Lotions Using High-Performance Liquid Chromatography” by Quiñones et al applies similar concepts, but HPLC was utilized for separation and students went further by creating a calibration curve to allow quantification of cannabinoids present in commercial lotions . In the paper “Chemistry of Cannabis: An Interdisciplinary Course”, Johnston describes the successful development and implementation of a course dedicated to cannabis chemistry .…”

Section: Introductionmentioning

confidence: 99%

Gas Chromatography–Mass Spectrometry Analysis of Cannabis: Undergraduate Organic Chemistry Laboratory Exercise

et al. 2023

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In this laboratory teaching experiment, commercial cannabis oils were analyzed by undergraduate organic chemistry students using gas chromatography–mass spectrometry (GC–MS) to investigate three isomeric cannabinoids commonly found in cannabis products: cannabidiol (CBD), Δ9-tetrahydrocannabinol (Δ9-THC), and Δ8-tetrahydrocannabinol (Δ8-THC). The goal was to introduce GC–MS analysis through the relevant topic of product quality inspection, frequently used in various industries such as pharmaceuticals, cosmetics, etc. and which largely involves analyzing the chemical components of a sample. First, to better understand the cannabinoid analytes, students were taught the differences between these structural isomers, including bond locations, stereochemistry, and functional groupsfactors which pertain to their pharmacology and drug classification. Finally, students were guided through the basics of GC–MS instrumentation and the process of analyzing GC–MS spectra through the characterization of CBD, Δ8-THC, and Δ9-THC present in oil samples.

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“…Forensics is an interesting science for students and teachers, in which many fields of applications can be found to explain several aspects of chemistry and analytical chemistry. − Students feel motivated when using Forensics since they face real world problems in cases surrounded by a sense of mystery. Besides, Forensic Chemistry is an interdisciplinary science involving many fields of knowledge such as drugs, , toxic materials, fibers, , fires and explosions, paints, ballistics, fingerprints, or documents, among others, in which a broad set of chemically different analytes can be determined by many types of analytical techniques, such as HPLC, − absorbance, or fluorescence . Specifically, questioned documents are of interest since they can be involved in cases of counterfeit currency, kidnappings, homicides, suicides, blackmail, and the verification of the authenticity of pieces of art, lottery tickets, or historical documentation. , …”

Section: Introductionmentioning

confidence: 99%

Inks in the Analytical Laboratory: A Forensics Activity for Undergraduate Students

Sáiz,

Rupérez-Pascualena

2023

J. Chem. Educ.

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Here, we present a laboratory activity in which the students work on an analysis of a questioned document that was written with one out of five possible pens. As a forensics study, the activity applied chemistry and analytical chemistry tools to solve the case. The students were able to apply polarity and solubility concepts to select and discard some pens. They also learned the basics of fluorescence and the possibilities of using it to detect falsifications. Then, they were able to set up the detection conditions for the HPLC-UV analysis by studying the absorbance behavior of the blue dyes. Finally, the students identified the pen used to write the suicide note and partially characterized the composition of the ink. This multipurpose activity perfectly suits subjects involving analytical techniques and forensics for students with basic knowledge of chemistry that are studying instrumental techniques for the first time.

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Analysis of Cannabinoids in Lotions Using High-Performance Liquid Chromatography

Cited by 8 publications

References 18 publications

Uncovering the Importance of Peer-to-Peer Communication during the COVID-19 Pandemic: Rotating Chromatography Experiments to Overcome Limited Student Capacity

Uncovering the Importance of Peer-to-Peer Communication during the COVID-19 Pandemic: Rotating Chromatography Experiments to Overcome Limited Student Capacity

Gas Chromatography–Mass Spectrometry Analysis of Cannabis: Undergraduate Organic Chemistry Laboratory Exercise

Inks in the Analytical Laboratory: A Forensics Activity for Undergraduate Students

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