Analysis of biosurfaces by neutron reflectometry: From simple to complex interfaces

Junghans, Ann; Watkins, Erik B.; Barker, Robert; Singh, Saurabh; Waltman, Mary Jo; Smith, Harold L.; Pocivavsek, Luka; Majewski, Jarosław

doi:10.1116/1.4914948

Cited by 35 publications

(23 citation statements)

References 100 publications

(102 reference statements)

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“…Deposition was carried out at a surface pressure held constant at 30 mN m −1 , following the procedure described previously for mixed, charged lipid systems 85, 86 . The blocks were assembled into low-volume measurement flow cells, to enable the in situ exchange of solvent and injection of protein samples 87 , and transferred directly to the neutron reflectometer for measurement.…”

Section: Methodsmentioning

confidence: 99%

Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line

Raasakka

Ruskamo

Kowal

et al. 2017

Sci Rep

158

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Compact myelin comprises most of the dry weight of myelin, and its insulative nature is the basis for saltatory conduction of nerve impulses. The major dense line (MDL) is a 3-nm compartment between two cytoplasmic leaflets of stacked myelin membranes, mostly occupied by a myelin basic protein (MBP) phase. MBP is an abundant myelin protein involved in demyelinating diseases, such as multiple sclerosis. The association of MBP with lipid membranes has been studied for decades, but the MBP-driven formation of the MDL remains elusive at the biomolecular level. We employed complementary biophysical methods, including atomic force microscopy, cryo-electron microscopy, and neutron scattering, to investigate the formation of membrane stacks all the way from MBP binding onto a single membrane leaflet to the organisation of a stable MDL. Our results support the formation of an amorphous protein phase of MBP between two membrane bilayers and provide a molecular model for MDL formation during myelination, which is of importance when understanding myelin assembly and demyelinating conditions.

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Section: Methodsmentioning

confidence: 99%

Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line

Raasakka

Ruskamo

Kowal

et al. 2017

Sci Rep

158

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“…The surface pressure was kept at a constant 30 mN m -1 during the deposition, as described previously (Barker et al 2016; Hubbard et al 2017; Raasakka et al 2017). All sample blocks were assembled into low-volume measurement flow cells, which were used for in situ exchange of solvent and injection of protein samples between reflectometric data collections (Junghans et al 2015).…”

Section: Methodsmentioning

confidence: 99%

Neuropathy-related mutations alter the membrane binding properties of the human myelin protein P0 cytoplasmic tail

Raasakka

Ruskamo

Barker

et al. 2019

Preprint

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“…We will introduce the different model membranes that can be employed, explaining the rationale, strengths and weaknesses for each one. The aim and scope of this chapter is somewhat different from exhaustive reviews of the field (Pabst et al 2010;Wacklin 2010;Junghans et al 2015).…”

Section: What Do We Want To Learn?mentioning

confidence: 99%

“…b Schematic of a solid/liquid reflectivity experiment in which the neutrons are incident through a single-crystal superphase, which forms one half of a laminar flow cell, which can be used to exchange the water sub-phase. Adapted from Junghans et al (2015) 3 At a fundamental level, the average (pseudo)potential experienced by the neutron is V ¼ 2p h 2 m q. Since the momentum change in specular reflection is solely perpendicular to the interface, it is the force on the neutron perpendicular to the interface that is important in determining the reflectivity.…”

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confidence: 99%

Neutron Reflectivity as a Tool for Physics-Based Studies of Model Bacterial Membranes

Barker

McKinley

Titmuss

2016

Biophysics of Infection

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The principles of neutron reflectivity and its application as a tool to provide structural information at the (sub-) molecular unit length scale from models for bacterial membranes are described. The model membranes can take the form of a monolayer for a single leaflet spread at the air/water interface, or bilayers of increasing complexity at the solid/liquid interface. Solid-supported bilayers constrain the bilayer to 2D but can be used to characterize interactions with antimicrobial peptides and benchmark high throughput lab-based techniques. Floating bilayers allow for membrane fluctuations, making the phase behaviour more representative of native membranes. Bilayers of varying levels of compositional accuracy can now be constructed, facilitating studies with aims that range from characterizing the fundamental physical interactions, through to the characterization of accurate mimetics for the inner and outer membranes of Gram-negative bacteria. Studies of the interactions of antimicrobial peptides with monolayer and bilayer models for the inner and outer membranes have revealed information about the molecular control of the outer membrane permeability, and the mode of interaction of antimicrobials with both inner and outer membranes.

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Analysis of biosurfaces by neutron reflectometry: From simple to complex interfaces

Abstract: Articles you may be interested inOne directional polarized neutron reflectometry with optimized reference layer method J. Appl. Phys. 112, 054301 (2012)

Cited by 35 publications

References 100 publications

Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line

Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line

Neuropathy-related mutations alter the membrane binding properties of the human myelin protein P0 cytoplasmic tail

Neutron Reflectivity as a Tool for Physics-Based Studies of Model Bacterial Membranes

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