Analysis of Beta-lactamase production and Antibiotics resistance in Staphylococcus aureus strains

Torimiro, Nkem; Aa, Moshood; Sa, Eyiolawi

doi:10.5897/jidi2013.0118

Cited by 12 publications

(6 citation statements)

References 18 publications

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“…The production of β-lactamase observed in this study was 49.4% in both wound and nasal S. aureus strains but lower compared to previous studies in which 70-80% was reported (Akindele et al, 2010;Torimiro et al, 2013). This difference may be as a result of the collection of samples from limited specimens compared to the previous investigators who collected samples from various clinical specimens.…”

Section: Discussioncontrasting

confidence: 55%

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Beta-lactamase Induction in Nosocomial Staphylococcus aureus Strains Cultured from the Nasal Cavity and Wound of Patients

Torimiro¹,

T²

2017

IJB

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The study was conducted to determine β-lactamase induction in Staphylococcus aureus strains cultured from the nasal cavity and surgical wounds of in-patients at a tertiary teaching hospital.Swab samples were taken from the nasal cavity -wound pair of 110 consented patients. The S. aureus isolates were identified and the antibiotics susceptibility tests were conducted using standard protocols. β-lactamase induction was assayed with the micro-iodometric method. The data obtained were statistically analysed.A total of 81 S. aureus isolates were cultured from the nasal cavity and wounds of the 110 samples collected. β-lactamase was produced by 14 (41.2%) and 26 (55.3%) of the isolates cultured from the nasal cavity and wounds respectively. Constitutive and induced β -lactamase production was observed.β-lactamase production in the isolates is not dependent on antibiotic exposure alone. The findings underscore the need for appropriate infection control and adequate hygiene practices to prevent the spread of S. aureus in the hospital settings.

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Section: Discussioncontrasting

confidence: 55%

“…observed to be over 80% has been documented (Parker & Collier, 1990;Schito, 2006;Akindele, Adedokun & Olaolu, 2010;Torimiro et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Beta-lactamase Induction in Nosocomial Staphylococcus aureus Strains Cultured from the Nasal Cavity and Wound of Patients

Torimiro¹,

T²

2017

IJB

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“…MRSA is resistant to entire classes of β -lactams including cephalosporins and carbapenems and has higher risk of development of resistance to quinolones, aminoglycosides, and macrolides [9–12]. …”

Section: Introductionmentioning

confidence: 99%

Detection of Methicillin ResistantStaphylococcus aureusand Determination of Minimum Inhibitory Concentration of Vancomycin forStaphylococcus aureusIsolated from Pus/Wound Swab Samples of the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal

Adhikari¹,

Pant

Neupane

et al. 2017

Canadian Journal of Infectious Diseases and Medical Microbiolog

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The present study was conducted to evaluate the performance of cefoxitin disc diffusion method and oxacillin broth microdilution method for detection of methicillin resistant S. aureus (MRSA), taking presence of mecA gene as reference. In addition, inducible clindamycin resistance and beta-lactamase production were studied and minimum inhibitory concentration (MIC) of vancomycin for S. aureus isolates was determined. A total of 711 nonrepeated pus/wound swab samples from different anatomic locations were included in the study. The Staphylococcus aureus was identified on the basis of colony morphology, Gram's stain, and biochemical tests. A total of 110 (15.47%) S. aureus isolates were recovered, of which 39 (35.50%) isolates were identified as MRSA by cefoxitin disc diffusion method. By oxacillin broth microdilution method, 31.82% of the Staphylococcus aureus isolates were found to be MRSA. However, mecA gene was present in only 29.1% of the isolates. Further, beta-lactamase production was observed in 71.82% of the isolates, while inducible clindamycin resistance was found in 10% of S. aureus isolates. The MIC value of vancomycin for S. aureus ranged from 0.016 μg/mL to 1 μg/mL. On the basis of the absolute sensitivity (100%), both phenotypic methods could be employed for routine diagnosis of MRSA in clinical microbiology laboratory; however cefoxitin disc diffusion could be preferred over MIC method considering time and labour factor.

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“…A capacidade hidrolítica da betalactamase depende de alguns fatores como sua localização, cinética, quantidade e condições físico-químicas (Livermore 2000). Além disso, o uso indiscriminado de antimicrobianos gera uma pressão seletiva para o aparecimento dessa resistência (McDougal & Thornsberry 1986, Livermore 2000, Torimiro et al 2013, Dias et al 2015.…”

Section: Staphylococcus Spp Resistentes à Meticilina (Mrs)unclassified

Mastite bovina causada por Staphylococcus spp. resistentes à meticilina: revisão de literatura

2018

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RESUMO.-Staphylococcus spp. são os micro-organismos mais relacionados a casos de mastite bovina. Algumas cepas destes micro-organismos têm apresentado fatores de virulência como genes de resistência a antimicrobianos com destaque para a resistência à meticilina que é um problema de saúde pública. Esta revisão de literatura tem o objetivo de compilar dados sobre a mastite bovina causada por Staphylococcus spp. resistente à meticilina (MRS). Apesar desse antimicrobiano não ser comumente utilizado no tratamento das mastites, a frequência de casos de infecção da glândula mamária causada por MRS tem variado entre 1,34 a 47,6%. Acredita-se que o contato dos humanos com animais positivos para MRS e vice-versa favoreça a transmissão deste patógeno entre as espécies, contribuindo para a variação nas taxas de infecção. A detecção de MRS pode ser realizada por meio de provas fenotípicas, moleculares ou sorológicas e as medidas de controle devem contemplar a identificação dos casos, segregação dos animais, estudo epidemiológico da fonte de infecção do rebanho, além da constante limpeza e higienização do ambiente de confinamento, equipamentos e utensílios de ordenha. Casos de mastite ocasionados por esse patógeno assumem relevância para a saúde pública, pois a ingestão de leite e/ou derivados contaminados podem desencadear a transferência de MRS para seres humanos. Com isso, é necessário um alerta constante quanto à vigilância epidemiológica em fazendas leiteiras. The most related microorganism in cases of bovine mastitis are Staphylococcus spp. Some strains of these microorganisms have shown virulence factors like antibiotic resistance genes, such as the resistance to methicillin, which represents a public health problem. This literature review aims to compile data related to bovine mastitis caused by Staphylococcus spp. Methicillin-resistant (MRS). Despite this antimicrobial not be commonly used in the treatment of mastitis, the frequency of cases of infection of the mammary gland caused by MRS has ranged from 1.34 to 47.6%. It is believed that the contact of humans with animals positive for MRS and vice versa favors the transmission of this pathogen among species, contributing to the variation in infection rates. MRS detection can be performed by phenotypic tests, molecular tests or serological tests and control measures must be taken such as the identification of cases, animal segregation, epidemiological study of the infection source of herd and the constant cleanliness and hygiene of the confined environment, equipment and milking utensils. Mastitis cases caused by this pathogen are of great relevance to public health because the ingestion of contaminated and/or derived from milk may trigger the transfer of MRS for human. Thus, a constant warning is required on the epidemiological surveillance in dairy farms.

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Analysis of Beta-lactamase production and Antibiotics resistance in Staphylococcus aureus strains

Cited by 12 publications

References 18 publications

Beta-lactamase Induction in Nosocomial Staphylococcus aureus Strains Cultured from the Nasal Cavity and Wound of Patients

Beta-lactamase Induction in Nosocomial Staphylococcus aureus Strains Cultured from the Nasal Cavity and Wound of Patients

Detection of Methicillin ResistantStaphylococcus aureusand Determination of Minimum Inhibitory Concentration of Vancomycin forStaphylococcus aureusIsolated from Pus/Wound Swab Samples of the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal

Mastite bovina causada por Staphylococcus spp. resistentes à meticilina: revisão de literatura

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