Analysis of Berberine Content using HPTLC Fingerprinting of Root and Bark of Three Himalayan Berberis Species

Andola, Harish Chandra; Rawal, Ranbeer S.; Rawat, Mahiraj Singh; Bhatt, Indra D.; Purohit, Vijay Kant

doi:10.3923/ajbkr.2010.239.245

Cited by 43 publications

(18 citation statements)

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“…As such no medicinal properties of the species are solely reported, however, it is used as a substitute of the B. aristata in the Himalayan region. Generally, roots are extracted for the various purposes as for other species of Berberis (Andola et al 2010). Natural regeneration of the species low and propagation through any means is not reported.…”

Section: Plant Materialsmentioning

confidence: 99%

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Influence of seed priming and storage time on germination and enzymatic activity of selected Berberis species

et al. 2015

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Effect of various priming treatments, storage conditions and seed collection sites on seed germination of selected species of Berberis i.e. B. aristata and B. jaeschkeana was studied. Pre-soaking the seeds in different treatments followed by incubation at 25/10°C (light: dark; 16:8 h) revealed variation in germination percentage, mean germination time (MGT), germination rate and seed vigour index. Seed treated with vermiwash (1:4 ratio) showed maximum germination percentage in B. aristata (92 %) and 1:10 ratio in B. jaeschkeana (50.6 %). Storage conditions (cold-dry for 1-4 months) and collection sites found to be effective for increasing germination percentage and germination rate in B. jaeschkeana at both the altitude (3200 and 3700 m asl) and B. aristata at higher altitude (2600 m asl) only. Further, germinated seedlings were analysed for enzymatic activity (Catalase and peroxidase) and found to be negatively correlated with catalase and positive with peroxidase activity for germination percentage in both species. Role of catalase and peroxidase in seed germination was associated with formation of reactive oxygen species which was found effective when treating with hydrogen peroxide. The potential for germination after various priming treatments especially vermiwash and thiourea is recommended and precursors such as hydrogen peroxide would be preferred in these species especially for reducing the MGT. Cold-dry storage would be preferred for Berberis species of higher altitude population.

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Section: Plant Materialsmentioning

confidence: 99%

“…B. jaeschkeana is distributed in entire Himalayan region between 3000-5200 m and considered endemic to Himalayan region (Andola et al 2010). The species is medicinally important and source of 'berberine'.…”

Section: Plant Materialsmentioning

confidence: 99%

Influence of seed priming and storage time on germination and enzymatic activity of selected Berberis species

et al. 2015

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“…The method can be used conveniently for the estimation of gallic acid and berberine in other herbal preparations and may be utilized for M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT standardization purpose. Its main advantages include its simplicity, accuracy and selectivity [37][38][39].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

A novel HPTLC method for quantitative estimation of biomarkers in polyherbal formulation

Sheikh¹,

Shakeel

Gul

et al. 2015

Asian Pacific Journal of Tropical Biomedicine

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“…[2,3,7,8] The percent entrapment was calculated using the formula. [9] %Entrapment efficiency = (amount of BE in sediment ÷ amount of BEin the extract added to ethosome)×100…”

Section: Entrapment Efficiencymentioning

confidence: 99%

“…The quantification and standardization of B. aristata extract have been carried out using its alkaloidal content as biomarker. [2,3] …”

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confidence: 99%

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Fatima

2017

AJP

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Aims: The aim of the present research work is to prepare ethosome of standardized Berberis aristata extract and to evaluate for vesicle shape, size, polydispersity index (PDI), zeta potential (ZP), entrapment efficiency (EE), and in vitro permeation studies. Materials and Methods: Ethosomes were prepared using soyaphosphatidylcholine (1-3%) and ethanol (10-40%) by modified cold method and were characterized. Results: The size of ethosomes were found to be in the range of 110.98-357.34 nm while PDI ranges from 0.114 to 1.56 and ZP was between −20.0 and −39.4 mV. Morphology studies showed unilamellar structure under transmission electron microscope and phase contrast microscope showed smooth surface. The EE of ethosomes was found to be in the range of 48.05% to 92.08%. Ethosomes were further added to carbopol 934P for gel formation, and subsequently, evaluated for their physicochemical properties. Discussion: In vitro diffusion study was conducted for ethosomal dispersion, hydroethanolic solution, ethosomes incorporated in viscous gel, and conventional gel using Franz diffusion cell for the biomarker compound berberine. Conclusions: It can be concluded that B. aristata loaded ethosomal delivery system is an encouraging approach for herbal drugs.

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Analysis of Berberine Content using HPTLC Fingerprinting of Root and Bark of Three Himalayan Berberis Species

Cited by 43 publications

References 9 publications

Influence of seed priming and storage time on germination and enzymatic activity of selected Berberis species

Influence of seed priming and storage time on germination and enzymatic activity of selected Berberis species

A novel HPTLC method for quantitative estimation of biomarkers in polyherbal formulation

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