A novel HPTLC method for quantitative estimation of biomarkers in polyherbal formulation

Sheikh, Zeeshan; Shakeel, Sadia; Gul, Somia; Zahoor, Aqib; Khan, Saleha Suleman; Zaidi, Faisal Haider; Usmanghani, Khan

doi:10.1016/j.apjtb.2015.06.016

Cited by 11 publications

(5 citation statements)

References 20 publications

(20 reference statements)

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“…The R f values of all three components, was found comparable in both sample and reference standard under UV light at 254 and 366 nm. It is reported in many studies that PE contains phytochemicals like gallic acid, ellagic acid, responsible for antioxidant, anti-inflammatory and immunomodulatory activities ( Sheikh et al, 2015 ). Similarly, quercetin is a flavonoid compound that possess anti-oxidant property ( Polu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Modulating effects of the synergistic combination of extracts of herbal drugs on cyclophosphamide-induced immunosuppressed mice

Parveen

Zahiruddin

Agarwal

et al. 2021

Saudi Journal of Biological Sciences

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Objective Taking leads from the available research, we aimed to develop a synergy-based herbal combination of Tinospora cordifolia (TC), Phyllanthus emblica (PE) , and Piper nigrum (PN) . Also, evaluating their synergistic effect on CP-induced immunosuppression in mice model and exploring the possible mechanisms involved in reversing the damage. Methodology The immunomodulatory activity of combination, of TC stem, PE fruits, and PN dried fruits, was determined by in vitro assays (splenocyte proliferation and pinocytic activity of peritoneal macrophages of mice) and in vivo study using CP-induced immunosuppression model in Swiss Albino mice. The ratio was optimized for combining three by in vitro MTT assay. The combination was further evaluated for anti-oxidant activity by DPPH scavenging method and quantified for its bioactive metabolites by HPTLC. Serum collected on day 0, 4, 7 and 14 was employed for estimation of haematogram (haematocrit, TLC, DLC, and haemoglobin, etc) and immune parameters (IL-10, IL-6 and TNF-α) by ELISA. Results The study demonstrated, that combination of herbal extracts at an intermediate dose could inhibit the proliferation of spleen cells and peritoneal macrophages (P ≤ 0.0001) and induce suppression of pro-inflammatory mediators, and also certified that combination exerts synergized effects. The results showed that the combination possess potential antioxidant activity by DPPH scavenging method (IC 50 -113.5 µg/ml). It was identified that combination significantly (P ≤ 0.0001) improved the immune markers, haematogram parameters, and histological parameters, with maximum protection offered by an intermediate dose. Conclusion The results suggested that present combination could be further explored clinically as potent synergy-based therapeutic approach for immune modulation.

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Section: Discussionmentioning

confidence: 99%

Modulating effects of the synergistic combination of extracts of herbal drugs on cyclophosphamide-induced immunosuppressed mice

Parveen

Zahiruddin

Agarwal

et al. 2021

Saudi Journal of Biological Sciences

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“…During the past few years, public interest in medicine obtained from plants has increased exponentially. According to the World Health Organization, 65%-80% of the mass population in developing countries due to lack of access to modern medicine depends basically on plants for primary healthcare needs 3 . Roses are a rich source of Vitamin C and are used in the making of medicaments.…”

Section: Introductionmentioning

confidence: 99%

A systematic review of the analytical method identified phytomedicine and pharmacological potency of Rosa species with special attention on Rosa centifolia

Singh,

Kumar,

Verma

et al. 2023

J. Drug Delivery Ther.

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Objective: Globally, Rosa species herbal plants are often used as a natural remedy to prevent and cure various health problems without or with minimal toxic effects. Rosa species are rich in bioactive phytochemicals such as flavonoids, polyphenols, tannins, and alkaloids. Phytomedicines are used to treat various diseases and disorders because of their clinical potency. Material and method: The searches were performed using various databases, including PubMed (http://www.ncbi.nlm.nih.gov/pubmed), Science Direct (http://www.sciencedirect.com/), Scopus (http://www.scopus.com/), and Google Scholar (http://www.scholar.google.com/). Result: India is well known for its geographical diversity, a rich collection of medicinally active plants, and numerous traditional herbal drug treatment systems that are acknowledged as “living traditions”. However, there is no collective report on the significant Rosa species with respect to the recent advancement in clinically active plant investigation. Hence, the key effort of the present review work is to report the identified phytomedicine and pharmacological activity majorly used Rosa species that have grown in India and other countries throughout the world. This review was completed on the basis of a complete national and international literature survey. Conclusion: In this review, we provide summarised and collective scientific information on the prominent Rosa species, used plant part, their extract, used analytical methods, biochemical and their respective medicinal activity to develop new ethnomedicine in the future. Keywords: Rosa species, analytical method, biochemical, pharmacological activity

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“…Several studies employing high-performance thin-layer chromatography (HPTLC) [15][16][17][18][19] and highperformance liquid chromatography (HPLC) [20][21][22][23] to estimate these markers alone or in combination have been published. However, a thorough review of the literature found that no validated HPTLC approach for simultaneously estimating all three markers has been published.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of HPTLC Method for Simultaneous Estimation of Berberine, Gallic Acid and Ursolic Acid in a Polyherbal Blend

Chitlange¹,

Chandani²,

Gandhi³

et al. 2022

Bull. of the Kar. Univ. "Chem". Ser.

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A sensitive High-performance thin-layer chromatography method was developed for simultaneous estimation of berberine, gallic acid and ursolic acid in a polyherbal blend and validated as per ICH guidelines.Polyherbal blend was prepared using widely recommended herbal plants for platelet augmentation activity viz. Carica papaya, Berberis aristata, Ocimum Sanctum and Tinospora Cordifolia. The optimized separation was obtained with TLC aluminum plates pre-coated with silica gel G60 F254 as a stationary phase and a solvent system containing Toluene : Ethyl acetate : Methanol : Formic acid (3:3:0.2:0.1 v/v/v/v). Berberine and gallic acid were found to demonstrate linearity in the range of 1μg/band – 6μg/band and ursolic acid in the range of 20 μg/band – 100 μg/band with the regression coefficient in acceptable limits. The method was also found to be specific and precise one. The accuracy of the developed method at 80 %, 100 % and 120 % levels was found to be within limits and % RSD was found to be less than 2. LOD and LOQ of all three standards were also determined. Blend was also quantified for the amount of berberine, gallic acid and ursolic acid presence and was found to be 37.8 μg, 46.1 μg and 108 μg per mg, respectively.

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A novel HPTLC method for quantitative estimation of biomarkers in polyherbal formulation

Cited by 11 publications

References 20 publications

Modulating effects of the synergistic combination of extracts of herbal drugs on cyclophosphamide-induced immunosuppressed mice

Modulating effects of the synergistic combination of extracts of herbal drugs on cyclophosphamide-induced immunosuppressed mice

A systematic review of the analytical method identified phytomedicine and pharmacological potency of Rosa species with special attention on Rosa centifolia

Development and Validation of HPTLC Method for Simultaneous Estimation of Berberine, Gallic Acid and Ursolic Acid in a Polyherbal Blend

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