Analysis of baculovirus aggregates using flow cytometry

Jorio, Hasnaa; Tran, Rosa; Meghrous, Jamal; Bourget, Lucie; Kamen, Amine

doi:10.1016/j.jviromet.2005.11.009

Cited by 29 publications

(25 citation statements)

References 20 publications

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“…Enveloped viruses are known to be sensitive to low H 3 O + activities (Gaudin et al, 1995). Also, pH changes resulting in virus particle aggregation have been correlated previously with decreased baculovirus infectivity (Jorio et al, 2006b). (Vicente et al, 2010).…”

Section: Net-surface Charge Of the Virus Particle And Aggregationmentioning

confidence: 84%

Purification of a recombinant baculovirus of Autographa californica M nucleopolyhedrovirus by ion exchange membrane chromatography

Grein

Michalsky

López

et al. 2012

Journal of Virological Methods

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Section: Net-surface Charge Of the Virus Particle And Aggregationmentioning

confidence: 84%

Purification of a recombinant baculovirus of Autographa californica M nucleopolyhedrovirus by ion exchange membrane chromatography

Grein

Michalsky

López

et al. 2012

Journal of Virological Methods

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“…A similar result was reported with baculovirus aggregates, which are categorized as nanoparticles. 44 In addition, the SSC histograms showed that nanoparticles had homogeneous granularity. This result was confirmed by the presence of a single narrow M1 peak.…”

Section: Discussionmentioning

confidence: 99%

Subacute toxicity of cadmium on hepatocytes and nephrocytes in the rat could be considered as a green biosynthesis of nanoparticles

Trabelsi¹,

Azzouz²,

Sakly³

et al. 2013

IJN

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Abstract:The purpose was to study the toxicity of cadmium (Cd) and to explore its potential to generate nanoparticles during detoxification. In order to demonstrate this, in vivo fluorescence imaging, X-ray diffraction, and flow cytometry were performed. The in vivo imaging showed a fluorescence signal after Cd treatment (CdCl2, 1.50 mg/Kg, intraperitoneally). By contrast, the control-rat fluorescence was negative. The fluorescence was divided into three colors, red, yellow, and green, and probably indicates the presence of quantum dots. X-ray diffraction results revealed the presence of Cd sulfide (CdS) and/or Cd selenide (CdSe) nanoparticles following Cd injection in the liver (6.52 nm) and kidneys (56.30 nm). Interestingly, flow cytometry revealed a heterogeneous size distribution and a homogeneous granularity of synthesized nanoparticles. Using the green fluorescence channel and the red fluorescence channel, a narrow green emission spectrum and a broad red emission spectrum were detected, respectively, by cytometric analysis.

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“…In the first case, the development of ELISA kits or other similar tests based on the immune detection of OBs (through the use of polyclonal or monoclonal antibodies against polyhedrin or granulin proteins) has standardized the quantitation of baculovirus allowing a more reliable measure (Parola et al, 2003). The use of flow cytometers also provides good results, but only so far for the quantification of BVs (Shen et al, 2002;Jorio et al, 2006). Once quantified the production of OBs, should determine their biological activity.…”

Section: Phenotype Quality Controlsmentioning

confidence: 99%