Analysis of bacterial communities in and on concrete

Maresca, Julia A.; Moser, Paul K.; Schumacher, Thomas

doi:10.1617/s11527-016-0929-y

Cited by 20 publications

(29 citation statements)

References 56 publications

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“…Phylum Proteobacteria represented the most abundant group in mortars with or without biosurfactant, followed by Firmicutes, Actinobacteria, Bacteroidetes, and Gemmatimonadetes. ese results agree with those of a previous study using DNA sequencing and classical cultivation methods to characterize the microbial communities in and on concrete, in which Actinobacteria and Proteobacteria were the most abundant groups [41]. Biosurfactant application noticeably reduced the relative abundance of Proteobacteria from 74.77% in the control group to around 56.14% in samples with 0.5% added biosurfactant, whereas the relative abundance of Actinobacteria increased from 15.76% in the control group to 28.76% in samples with 0.5% added biosurfactant.…”

Section: Characterization Of Bacterial Diversity Inside Mortarssupporting

confidence: 91%

Modifying Mechanical Strength and Capillary Porosity of Portland Cement-Based Mortar Using a Biosurfactant from Pseudomonas fluorescens

Serres

Meylheuc

et al. 2020

Advances in Materials Science and Engineering

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We characterized the effects of a biosurfactant derived from Pseudomonas fluorescens on slump loss, mechanical strength, capillary porosity, and bacterial colonization inside Portland cement-based mortar samples. Standard tests were used to evaluate the utility of this biosurfactant as an admixture. e addition of 1.5% biosurfactant increased the plasticity and improved the workability of fresh samples. Although compressive and flexural strengths of mortars with biosurfactant were lower than those of mortars without biosurfactant after a short curing period (28 days), the addition of biosurfactant increased the compressive strength of mortar after a long curing period (180 days), with 1% biosurfactant having the highest value. After 180 days, mortar with biosurfactant had significantly lower capillary absorption coefficient A values (P < 0.05) than mortar without biosurfactant. Furthermore, the addition of biosurfactant reduced the relative abundance of the mortar-deteriorating bacterial genus Pseudomonas (phylum Proteobacteria).

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Section: Characterization Of Bacterial Diversity Inside Mortarssupporting

confidence: 91%

Modifying Mechanical Strength and Capillary Porosity of Portland Cement-Based Mortar Using a Biosurfactant from Pseudomonas fluorescens

Serres

Meylheuc

et al. 2020

Advances in Materials Science and Engineering

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“…low cost, concrete is the most widely used building material in the world (1). It is, therefore, a very common environment, and despite the tough conditions, bacteria are known to live in and on concrete (2)(3)(4)(5)(6)(7)(8)(9)(10)(11).…”

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confidence: 99%

Bacterial Communities in Concrete Reflect Its Composite Nature and Change with Weathering

Keffer

Maresca

2021

mSystems

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Concrete is an extreme but common environment and is home to microbial communities adapted to alkaline, saline, and oligotrophic conditions. Microbes inside the concrete that makes up buildings or roads have received little attention despite their ubiquity and capacity to interact with the concrete. Because concrete is a composite of materials which have their own microbial communities, we hypothesized that the microbial communities of concrete reflect those of the concrete components and that these communities change as the concrete ages. Here, we used a 16S amplicon study to show how microbial communities change over 2 years of outdoor weathering in two sets of concrete cylinders, one prone to the concrete-degrading alkali-silica reaction (ASR) and the other having the risk of the ASR mitigated. After identifying and removing taxa that were likely laboratory or reagent contaminants, we found that precursor materials, particularly the large aggregate (gravel), were the probable source of ∼50 to 60% of the bacteria observed in the first cylinders from each series. Overall, community diversity decreased over 2 years, with temporarily increased diversity in warmer summer months. We found that most of the concrete microbiome was composed of Proteobacteria, Firmicutes, and Actinobacteria, although community composition changed seasonally and over multiyear time scales and was likely influenced by environmental deposition. Although the community composition between the two series was not significantly different overall, several taxa, including Arcobacter, Modestobacter, Salinicoccus, Rheinheimera, Lawsonella, and Bryobacter, appear to be associated with ASR. IMPORTANCE Concrete is the most-used building material in the world and a biologically extreme environment, with a microbiome composed of bacteria that likely come from concrete precursor materials, aerosols, and environmental deposition. These microbes, though seeded from a variety of materials, are all subject to desiccation, heating, starvation, high salinity, and very high pH. Microbes that survive and even thrive under these conditions can potentially either degrade concrete or contribute to its repair. Thus, understanding which microbes survive in concrete, under what conditions, and for how long has potential implications for biorepair of concrete. Further, methodological pipelines for analyzing concrete microbial communities can be applied to concrete from a variety of structures or with different types of damage to identify bioindicator species that can be used for structural health monitoring and service life prediction.

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“…We previously isolated several bacterial strains from concrete, an alkaline, high-salt environment ( 1 ), and then sequenced the genome of Rhodococcus sp. strain CL-05 to compare it with those of alkaliphilic and halophilic Rhodococcus species ( 2 – 4 ).…”

Section: Announcementmentioning

confidence: 99%

“…Pieces of concrete (∼1 g) were vortexed in TE buffer (10 mM Tris, 1 mM EDTA), and then 25 μl of solution was spread onto concrete medium solidified with agar (CM-A) and incubated at room temperature for ∼2 weeks ( 1 ). Individual colonies were restreaked onto CM-A until axenic, as determined by microscopy.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of Rhodococcus qingshengii Strain CL-05, Isolated from Concrete

McDermott

Шевченко

Ross

et al. 2021

Microbiol Resour Announc

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Analysis of bacterial communities in and on concrete

Cited by 20 publications

References 56 publications

Modifying Mechanical Strength and Capillary Porosity of Portland Cement-Based Mortar Using a Biosurfactant from Pseudomonas fluorescens

Modifying Mechanical Strength and Capillary Porosity of Portland Cement-Based Mortar Using a Biosurfactant from Pseudomonas fluorescens

Bacterial Communities in Concrete Reflect Its Composite Nature and Change with Weathering

Complete Genome Sequence of Rhodococcus qingshengii Strain CL-05, Isolated from Concrete

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